Gold and silica gold nanoparticles enhances macrophages kill tumor cells via granzyme-perforin pathway

Salah H. Mannoush; Majid S. Jabir; Abid A. Thaker

doi:10.53730/ijhs.v6nS6.12272

Authors

Salah H. Mannoush
salah.bio2015@uoanbar.edu.iq
Department of Biotechnology, College of Science, University of Anbar, Iraq
Majid S. Jabir Division of biotechnology, Department of applied science, University of Technology-Iraq
Abid A. Thaker Department of Biology, College of Science, University of Anbar, Iraq

Keywords:

GNPs, Si-GNPs, phagocytosis, SKOV-3, EAC, granzyme, perforin

Abstract

The goal of the study was to see if gold nanoparticles (GNPs) and silica gold nanoparticles (Si-GNPs) could help cancer cells phagocytes. Scanning electron microscopy (SEM) and Ultraviolet-visible spectroscopy (UV-VIS) absorptions were used to characterize the nanomaterial's. In both in vitro and in vivo conditions, the GNPs and Si-GNPs enhanced macrophage phagocytosis capabilities, resulting in the annihilation of cancer cells. The effect of GNPs and Si-GNPs in Tumor necrosis factor alpha (TNF-α) and Interleukin-8 (IL-8) levels was investigated in Ehrlich tumor cells using Enzyme linked immunosorbent assay (ELISA) assay. The results shows the ability of GNPs and Si-GNPs to increase TNF-α and IL-8 in treated Ehrlich Ascites Carcinoma (EAC). It was found that the most effect was in Si-GNPs group. GNPs and Si-GNPs enhance phagocytic cells for increased granzyme and perforin-mediated cancer cell death on a regular basis. Overall, the findings showed that GNPs and Si-GNPs improved phagocytic cell activation, which might be employed as a promising technique for managing cancer cells by enhancing cell death via a Granzyme-perforin dependent mechanism.

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