Analysis of the ZNT8, GAD65, HLA-DQA1, HLA-DQB1, and C-peptides in Indonesian children with type 1 diabetes mellitus

Nur Rochmah; Muhammad Faizi; Yuni Hisbiyah; Rayi Kurnia Perwitasari; Tyas Maslakhatien Nuzula; Anang Endaryanto; Soetjipto Soetjipto

doi:10.53730/ijhs.v6nS9.12857

Authors

Nur Rochmah Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
Muhammad Faizi Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
Yuni Hisbiyah Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
Rayi Kurnia Perwitasari Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
Tyas Maslakhatien Nuzula Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
Anang Endaryanto Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia
Soetjipto Soetjipto
soetjipto@fk.unair.ac.id
Faculty of Medicine, Department of Biochemistry, Universitas Airlangga, Surabaya, East Java, Indonesia

Keywords:

T1DM, peptide, children, antibody, diabetes

Abstract

Autoimmune reaction in Type 1 Diabetes Mellitus (T1DM) is caused by genetic and environmental factors. The genes HLA-DQA1 and HLA-DQB1 are strongly associated with T1DM. Other established markers of T1DM were Zinc Transporter 8 Antibody (ZnT8), Glutamic Acid Decarboxylase Antibody-65 (GAD65), and C-peptide. This study aims to analyze ZnT8, GAD65, HLA-DQA1, HLA-DQB1, and C-peptide in Indonesian children with T1DM. A cross-sectional study of T1DM children was conducted in Dr. Soetomo Hospital, Indonesia. ZnT8, GAD65, HLA-DQA1, HLA-DQB1, and C-peptide were examined with an ELISA kit. Mann-Whitney test was performed with a significant value of p<0.05. A total of 62 participants consisting of 31 children for each group were enrolled. The prevalence of ZnT8, GAD65, and C-peptide in T1DM patients was 77.4%, 90.3%, and 87.1%. There are 74% of T1DM children had two positive antibodies and 55% had two positive HLA peptides. There was no difference in HLA-DQA1 and HLA-DQB1 peptides between T1DM and control groups (p=0.12; p=0.19). Meanwhile, the comparison between ZnT8, GAD65, and C-Peptide values between T1DM and control groups shows a significant difference (p<0.05). HLA-DQA1 peptide value was higher in T1DM children and HLA-DQB1 peptide value was higher in non-T1DM children.

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