Effect of exposing the seeds of tomato plant (Solanum lycopersicum L.) to diode laser rays on the germination, growth of hypocotyl stems callus of their seedlings and its content of lycopene

https://doi.org/10.53730/ijhs.v6nS1.4844

Authors

  • Rana Tariq Yahya Department of Biophysics, College of Science, University of Mosul, Mosul, Iraq
  • Safwan Jasim Sultan Al-Noor University College, Bartella, Iraq

Keywords:

tomato plant (Solanum lycopersicum L.), diode laser rays, callus, seedlings, lycopene

Abstract

The current study succeeded in demonstrating the role of red laser beams at a wavelength of 650 nm and a power of 50 milliwatts / cm2 when exposed to the seeds of the tomato plant Solanum lycopersicum L. at times of 0,5,10,15 minutes on their germination rate and seedlings growth, and then estimating the growth of its callus and its content of lycopene. Also the results showed that the time period of 10 minutes exceeded others in encouraging seed germination with a full percentage (100%) after 5 days of planting them on solid Murashige and Skoog (MS) medium with distinct growth of its seedlings, whose roots and vegetative total lengths reached 3.9, 7.1 cm after 20 days compared to seeds not exposed to radiation, the percentage reached 80% after 7 days, with seedlings growing at a rate of 2.3 and 3.4 cm, respectively. The study was able to initiate callus by cutting stems of its sub cotyledon seedlings on MS medium supplemented with 2.0 mg /l TDZ and 0.5 mg/l NAA.

Downloads

Download data is not yet available.

References

Shengwei Z and Jingsam, S. Rapid plant regeneration from cotton(Gossypium hirsutum L.). Chin. Sci. Bulletin. 2000 ; 45(19):1772-1773.

Kazemzadeh - Beneh H, Mahna N, Safari E, Zaare-Nahandi F and Motallebi-Azar A. Effects of diode and He-Ne laser on in vitro production of anthocyanin in apple cell suspension culture. Internat. J. Horticul. Sci. Technol. 2015; 2(2): 205-212.

El-Sherif F, Yap YK and Ibrahim HI. Laser irradiation induces DNA polymorphism and alters phytochemicals compositions as well as growth and yield of Curcuma longa. J. Diseases and Medic. Plants. 2019; 5 (2) : 29-38.

Cope KR and Bughee B. Spectral effects of three types of white light emitting diodes on plant growth and development: Absolute versus relative amounts of blue light. Hort. Sci. 2013; 48(4):504-509.

Kazemzadeh -Beneh H, Mahna N, Safari E and Motallebi - Azar A. Blue diode and red He-Ne lasers affect the growth of anthocyanin producing suspension cells of apple. Internat. J. Horticult. Sci. Technol. 2018; 5(2):231-239.

Krawiec M , Dziwulska-Hunek A , Palonka S , Kap?an M and Bary?a P. Effect of laser irradiation on seed germination and root yield of scorzonera (Scorzonera hispanica L.). Acta Agroph., 2016 ; 23(4): 621-631.

Ghanem S N. Effect of laser radiation in the initiation and growth of sunflower (Helianthus annus L.) calli , dihydrofolate reductase enzyme activity and their contents nucleic acids, proteins and folate. 2017; M.Sc Thesis, College of Science, University of Mosul, Iraq.

Shi J and Mageur LM. Lycopene in tomatoes; chemical and physical properties affected by food processing. Crit. Rev. Food Sci. Nutr. 2000 ; 40: 1-42.

Zuorro A. Enhanced lycopene extraction from tomato peels by optimized mixed-polarity solvent mixtures. Molecules, 2020; 25(9): p.2038.

Al- Taee N E J, Mohammed A A H and Ahmed J M Y. Production of transgenic tomato (Lycopersicon esculetum) plants by Agrobacterium tumefaciens C58C1 mediated transformation. Internat. J. Sci. Technol., 2015; 10(2):105-110.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962; 15: 473-497.

Osman MG, Elhadi EA and Khalafalla MM .Callus formation and organogenesis of tomato (Lycopersicon esculentum Mill, C.V. Omdurman) induced by thidiazuron. African J. Biotech. 2010, 9(28):4407-4413.

Fish WW, Perkins-Veazie P and Collins JK.. A quantitative assay for lycopene that utilizes reduced volumes of organic solvents. J. Food Comp. Anal., 2002; 15, 309 -/317.

Metwally SA. Physiological and anatomical studies on the effect of gamma and laser irradiation and some bio regulators treatments on the growth, flowering and keeping quality of gerbera. 2010; Ph.D. Thesis, Faculty of Agriculture, Zagazig University.

Ri P and Jang Y.Study on laser pre-sowing treatment of rice seeds by free- falling method, IPA. 2019; 6 (4) : 515-521.

Klimek-Kopyra A , D?u?niewska J , ?lizowska A and Dobrowolski J W. Impact of coherent laser irradiation on germination and mycoflora of soybean seeds—innovative and prospective seed quality management. Agriculture, 2020 ; 10(8): 314.

Samuilov FD and Garifullina RL. Effect of laser irradiation on micro-viscosity of aqueous medium in imbibing maize seeds as studied with a spin probe method. Russ. J. Plant Physiol. 2007 ; 54(1): 128-131.

Kamiya H and Ozawa S. Dual mechanism for presynaptic modulation by axonal metabotropic glutamate receptor at the mouse mossy fibre-CA3 synapse. J. Physiol., 1999 ; 15 (2): 497–506.

Shilpa AK and Kaur R . Establishment and regeneration of callus cultures in tomato (Solanum lycopersicum L.) from various explants. Ann. Res. Rev. Biol., 2017; 12(2): 1-6.

Ghanem S N and Abood SA. Diode laser radiation effects in initiation and growth of sun flower plant (Helianthus annuus L.) callus. Rafidain J. Sci., 2019; 28(1): 24-34.

Yaseen E T and Abood SA. Role of diode laser radiation pretreatments in growth of Pisum sativum L. plant seedlings and callus under salinity stress. Rafidain J.Sci. , 2020 ; 29(1):50-67.

Janayon RVB and Guerrero RA. Laser irradiation of mung bean (Vigna radiata L.) at two wavelengths for enhanced seedling development. Internat. J. Optics. 2019 ; 3:1-7.

Pizarro L and Stange C . Light-dependent regulation of carotenoid biosynthesis in plants. Cien. Inv. Agr., 2009 ; 36:143-162.

Bao-xing X, Jing-jing W, Yi-ting Z , Shi-wei S, Wei S, Guang-wen S, Yan-wei H and Hou-cheng L. Supplemental blue and red light promote lycopene synthesis in tomato fruits. J. Integrative Agricult.,2019; 18(3):590–598.

Leivar P and Monte EP. Systems integrators in plant development. Plant Cell., 2014 ; 26(1):56-78.

Published

18-03-2022

How to Cite

Yahya, R. T., & Sultan, S. J. (2022). Effect of exposing the seeds of tomato plant (Solanum lycopersicum L.) to diode laser rays on the germination, growth of hypocotyl stems callus of their seedlings and its content of lycopene. International Journal of Health Sciences, 6(S1), 987–996. https://doi.org/10.53730/ijhs.v6nS1.4844

Issue

Section

Peer Review Articles