Effect ropivacaine infiltration in incisional wound on caspase-3 expression and apoptosis squamous cells wound healing
Keywords:
Ropivacaine, Local Anesthesia, Caspase 3, Apoptosis, Wound HealingAbstract
Wound healing and tissue regeneration consist of molecular and cellular process that occur right after wound formation. The intrinsic pathway of apoptosis, apoptosome produced to initiate the formation of Caspase 3 important role in apoptosis. One of the methods that accelerates wound healing process by eliminating pain by infiltration of local anesthesia. This study aims to determine the effect of ropivacaine infiltration as local anaesthesia in the expression of Caspase 3 and apoptosis. This study is an experimental study with randomized post-test only control group design. The samples of this study were 24 wistar rats. The rats divided into four groups then incised, either followed by 1 ml ropivacaine 0.2% infiltration or left as is. Excisional biopsy procedures were done on third and seventh day. Then analyzed by SPSS. Expression of Caspase 3 on squamous cell epithel of control and intervention group was found statistically different on day 3 (p=0.024) and day 7 (p=0.041). Number of apoptosis on squamous cell epithel of control and intervention was found statistically different on day 7 (p=0.031) but not on day 7 (p=0.414). Ropivacaine infiltration on incision wound decreased the expression of Caspase 3 on day 3 and 7; and apoptosis on day 7.
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Abrão, J., Antunes, M., & Garcia, L. V. (2020). Local Anesthetics Infiltration and Wound Healing Process. Topics in Local Anesthetics, 119. http://dx.doi.org/10.5772/intechopen.80137
Brower, M. C., & Johnson, M. E. (2003). Adverse effects of local anesthetic infiltration on wound healing. Regional anesthesia and pain medicine, 28(3), 233. Available from: https://www.proquest.com/openview/a4fb4cc892030895cfd928fe749f70b7/1?pq-origsite=gscholar&cbl=47693
Enoch, S., & Leaper, D. J. (2008). Basic science of wound healing. Surgery (Oxford), 26(2), 31-37.
Ferreira, K. S., Kreutz, C., MacNelly, S., Neubert, K., Haber, A., Bogyo, M., ... & Borner, C. (2012). Caspase-3 feeds back on caspase-8, Bid and XIAP in type I Fas signaling in primary mouse hepatocytes. Apoptosis, 17(5), 503-515. https://doi.org/10.1007/s10495-011-0691-0
Gonzalez, A. C. D. O., Costa, T. F., Andrade, Z. D. A., & Medrado, A. R. A. P. (2016). Wound healing-A literature review. Anais brasileiros de dermatologia, 91, 614-620. https://doi.org/10.1590/abd1806-4841.20164741
Hancı, V., Hakimoğlu, S., Özaçmak, H., Bektaş, S., Özaçmak, H. S., Özdamar, Ş. O., ... & Turan, I. Ö. (2012). Comparação dos efeitos da infiltração com bupivacaína, lidocaína e tramadol na cicatrização de feridas em ratos. Revista Brasileira de Anestesiologia, 62(6), 804-810. https://doi.org/10.1590/S0034-70942012000600005
Hollmann, M. W., Durieux, M. E., & Fisher, D. M. (2000). Local anesthetics and the inflammatory response: a new therapeutic indication?. The Journal of the American Society of Anesthesiologists, 93(3), 858-875. https://doi.org/10.1097/00000542-200009000-00038
Maae, E., Nielsen, M., Steffensen, K. D., Jakobsen, E. H., Jakobsen, A., & Sørensen, F. B. (2011). Estimation of immunohistochemical expression of VEGF in ductal carcinomas of the breast. Journal of Histochemistry & Cytochemistry, 59(8), 750-760. https://doi.org/10.1369/0022155411412599
Pramono, W. B., Leksana, E., & Satoto, H. H. (2016). Pengaruh pemberian ropivakain infiltrasi terhadap tampilan kolagen di sekitar luka insisi pada tikus Wistar. JAI (Jurnal Anestesiologi Indonesia), 8(1), 1-10.https://doi.org/10.14710/jai.v8i1.11859.
Zoellner, H., Hofler, M., Beckmann, R., Hufnagl, P., Vanyek, E., Bielek, E., ... & Binder, B. R. (1996). Serum albumin is a specific inhibitor of apoptosis in human endothelial cells. Journal of cell science, 109(10), 2571-2580. https://doi.org/10.1242/jcs.109.10.2571
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