Phytochemical studies on cyanidin 3-O glucoside•HCl from the ripened fruit pulp of Terminalia catappa

Kranti Kamble; K. S. Laddha

doi:10.53730/ijhs.v6nS4.11689

Authors

Kranti Kamble Research Scholar, Department of Pharmaceutical Sciences, Institute of Chemical Technology, Mumbai, Maharashtra, India
K. S. Laddha
ks.laddha@ictmumbai.edu.in
Research Guide, Department of Pharmaceutical Sciences, Institute of Chemical Technology, Mumbai, Maharashtra, India

Keywords:

anthocyanin, cyanidin-3-o-glucoside, total phenolic content, antioxidant activity, Terminalia catappa

Abstract

Context :Terminalia catappa (Combretaceae) is a large tropical tree grows mainly in the tropical regions of Asia, Africa, and Australia. The fruits a drupe 5-7 cm long and 3-5.5 cm broad, green at first, then yellow, and finally red when ripe, containing a single seed. Ripened fruit pulp contains anthocyanins. Objectives: To extract and isolate active anthocyanin from fruit pulp of Terminalia catappa. And characterize isolated cyanidin-3-0-glucoside by spectral analysis.Methods: An easy and efficient technique for isolating and extracting anthocyanin from Terminalia catappa ripen fruit pulp. Besides the total phenolic, flavonoid, anthocyanin, and reducing sugar content of the extract, the antioxidant capacity of T.catappa fruit pulp was evaluated and by DPPH, H2O2 methods. Characterization of cyanidin-3-o-glucoside done by UV, IR spectral analysis. While single crystal structure and elemental detection done by EDS analysis. And further structural confirmation done by LCMS analysis.Result: Fresh extract contain 26.17 % and isolated compound contain 83.79% total anthocyanin content, the total phenolic content was found to be 1.58 % in fresh extract as compared with gallic acid standard. 34.48 % total reducing sugar content and 54.50 % total flavonoid content observed.

Downloads

Download data is not yet available.

References

Anand, A., Divya, N., &Kotti, P. (2015). An updated review of Terminalia catappa. Pharmacognosy reviews, 9(18), 93.

Anuforo, P. C., Achi, N. K., Egbuonu, A. C. C., &Egu, E. U. (2018). Determination of some phyto-constituents present in Terminalia catappa endocarp flour and biochemical evaluation of its ethanol extract on hepatic indices in male wistar rats. Journal of Pharmacology and Toxicology, 13(1), 27-36.

Ayina, K., Hema, A., Dabiré, C., Palé, E., Duez, P., Luhmer, M., &Nacro, M. (2015). Major anthocyanin structure elucidation and radical-scavenging activities of total anthocyanins extract from Terminalia catappa red leaves. ChemXpress, 8(1). 48-55.

Bhatti, M. Z., Ali, A., Ahmad, A., Saeed, A., & Malik, S. A. (2015). Antioxidant and phytochemical analysis of Ranunculusarvensis L. extracts. BMC research notes, 8(1), 1-8.

Castañeda-Ovando, A., de Lourdes Pacheco-Hernández, M., Páez-Hernández, M. E., Rodríguez, J. A., &Galán-Vidal, C. A. (2009). Chemical studies of anthocyanins: A review. Food chemistry, 113(4), 859-871.https://doi.org/10.1016/j.foodchem.2008.09.001

Chauhan, A., & Samant, S. A. (2022). Isolation and identification of kurthiagibsonii from paneer and study its antibacterial activity against intestinal pathogens. International Journal of Health Sciences, 6(S5). https://doi.org/10.53730/ijhs.v6nS5.11269

Favaro, L. I., Balcão, V. M., Rocha, L. K., Silva, E. C., Oliveira Jr, J. M., Vila, M. M., &Tubino, M. (2018). Physicochemical characterization of a crude anthocyanin extract from the fruits of Jussara (Euterpe edulis Martius): potential for food and pharmaceutical applications. Journal of the Brazilian Chemical Society, 29, 2072-2088.

Fedenko, V. S., Shemet, S. A., &Landi, M. (2017). UV–vis spectroscopy and colorimetric models for detecting anthocyanin-metal complexes in plants: An overview of in vitro and in vivo techniques. Journal of plant physiology, 212, 13-28. http://dx.doi.org/doi:10.1016/j.jplph.2017.02.001.

Garzón, G. A., Riedl, K. M., & Schwartz, S. J. (2009). Determination of anthocyanins, total phenolic content, and antioxidant activity in Andes berry (RubusglaucusBenth). Journal of food science, 74(3), C227-C232.

Kaneria, M. J., Rakholiya, K. D., Marsonia, L. R., Dave, R. A., &Golakiya, B. A. (2018). Nontargeted metabolomics approach to determine metabolites profile and antioxidant study of Tropical Almond (Terminalia catappa L.) fruit peels using GC-QTOF-MS and LC-QTOF-MS. Journal of Pharmaceutical and Biomedical Analysis, 160, 415-427.

Krishnaveni, M., JasbinShyni, G., Dhanalakshmi, R., Magesh, P., Ponraj, K., Lavanya, K., &Kalimuthu, R. (2014). A preliminary study on phytoanalysis, Antioxidant potential of Terminalia catappa l. fruit flesh. International Journal of Pharmaceutical Sciences Review and Research, 28(1), 83-87.

Lee, J., Durst, R. W., Wrolstad, R. E., & Collaborators: Eisele T Giusti MM Hach J Hofsommer H Koswig S Krueger DA Kupina; S Martin SK Martinsen BK Miller TC Paquette F Ryabkova A Skrede G Trenn U Wightman JD. (2005). Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC international, 88(5), 1269-1278.

Ondo, J. P., Obame, L. C., Akoue, G. N., Engono, J. P. M., Parkouda, S., Emvo, E. N., &Lebibi, J. (2013). Studies on phytochemical screening, total phenolic content and antiradical activity of three extracts of Emilia sagittata DC.(Asteraceae). International Journal of Biosciences (IJB), 3(4), 50-57.

Panda, S., & Das, S. (2022). Extraction, isolation and quantification of curcumin for possible diabetis treatment. International Journal of Health Sciences, 6(S4). https://doi.org/10.53730/ijhs.v6nS4.10994

Phuyal, N., Jha, P. K., Raturi, P. P., &Rajbhandary, S. (2020). Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylumarmatum DC. The Scientific World Journal, 2020., 7.

Rakkimuthu, R., Palmurugan, S., &Shanmugapriya, A. (2016). Effect of temperature, light, pH on the stability of anthocyanin pigments in cocculushirsutus fruits. International Journal of Multidisciplinary Research and Modern Education, 2(2), 91-96.

Sekar, A., Pandian, A., Veerabathiran, R., Mariappan, R., Chelladurai, G., &Sivamani, G. (2022). Antidiabetic effects of marine brown algae Spatoglossumasperum J. Ag. ethanolic extract on 3T3-L1 Pre-Adipocytes activity an in vitro approach. International Journal of Health Sciences, 6(S4). https://doi.org/10.53730/ijhs.v6nS4.11408

Thomson, L. A., & Evans, B. (2006). Terminalia catappa (tropical almond). Species Profiles for Pacific Island Agroforestry, 2(2), 1-20.

Trikas, E. D., Papi, R. M., Kyriakidis, D. A., &Zachariadis, G. A. (2016). A sensitive LC-MS method for anthocyanins and comparison of byproducts and equivalent wine content. Separations, 3(2), 18.

Venkatalakshmi, V. Brindha.(2016). Phytopharmacological Significance of Terminalia catappa L.: an Updated Review. International Journal of Research in Ayurveda & Pharmacy. https://doi.org/10.7897/2277-4343.07272.

Yang, H., Kim, H. W., Kwon, Y. S., Kim, H. K., & Sung, S. H. (2017). Fast and Simple Discriminative Analysis of Anthocyanins‐Containing Berries Using LC/MS Spectral Data. Phytochemical Analysis, 28(5), 416-423.