Treatment of 40 kHz continuous ultrasound towards blood cells of mice (Mus musculus L)
The application of ultrasound in mice has increased the activity of mice and showed anxious behavior that affects the blood cells of mice. This study aims to observe the effect of 40 kHz ultrasound exposure on the content of leukocytes and erythrocytes in blood cells of mice. For this purpose, five ultrasound treatments with sound pressure levels were 70, 75, 80, 85, and 90 dB respectively and one control in six sample groups, each consisting of five mice with the same weight and age. Sound treatment is carried out with a 40 kHz continuous ultrasound exposure of the same duration for two hours. Taking blood samples of mice is carried out as soon as the sample is given a sound treatment. Based on the results of blood tests of mice it was found that the average increase in the number of leukocyte mice varied between 40-1,070 cells/mm3 for variations in sound pressure levels from 70 to 90 dB with an increase in the highest average number of leukocyte (1,070 cells/mm3) at the sound pressure level 75 dB. With the same level of sound pressure level, the decrease in the average number of erythrocytes varied between 920,000-190,000 cells/mm3 with a decrease in the highest average number of erythrocytes (920,000 cells/mm3) at an 80 dB sound pressure level. The optimum level of sound pressure that can affect the average number of leukocytes and erythrocytes in blood cells of mice are still unknown.
Ackerman, E., Ellis, L. B. M., Williams, L. E. (1979). Biophysical Science. New Jersey, Prentice-Hall, (Chapter F).
Dahlan, Santoso, K., Bahri, S. (2007). Pengaruh Gelombang Bunyi Audiosonik dan Ultrasonik pada Aktivitas Mencit. Jurnal Biofisika, 3(1), 19-28.
Halliday, D., Resnick, R., & Walker, J. (2010). Fundamentals of Physics, Chapters 33-37. John Wiley & Sons. https://books.google.com/books?hl=en&lr=&id=aO-Xrlje7hMC&oi
Harle, J., Salih, V., Mayia, F., Knowles, J. C., & Olsen, I. (2001). Effects of ultrasound on the growth and function of bone and periodontal ligament cells in vitro. Ultrasound in medicine & biology, 27(4), 579-586. https://doi.org/10.1016/S0301-5629(00)00326-4
Harris, N. C., Hemmerling, E. M., Mallmann, A. J. (1995). Physics: Principles and Applications, New York: Glencoe, McGraw-Hill, (Chapter 5).
Heffner, H. E., & Heffner, R. S. (2007). Hearing ranges of laboratory animals. Journal of the American Association for Laboratory Animal Science, 46(1), 20-22. https://www.ingentaconnect.com/content/aalas/jaalas/2007/00000046/00000001/art00003
Jalali, M., Saki, G., Sarkaki, A. R., Karami, K., & Nasri, S. (2012). Effect of noise stress on count, progressive and non-progressive sperm motility, body and genital organ weights of adult male rats. Journal of human reproductive sciences, 5(1), 48. https://dx.doi.org/10.4103%2F0974-1208.97801
Ogochukwu, E. S., Okechukwu, A. D., & Nnaegbo, O. G. Construction And Testing Of Ultrasonic Bird Repeller.
Parker, S. P. (1988). Acoustics source book. New York: McGraw-Hill.
Simeon, M. I., Mohammed, A. S., & Adebayo, S. E. (2013). Development and preliminary testing of an electronic pest repeller with automatic frequency variation. http://dspace.futminna.edu.ng/jspui/handle/1/4511
Copyright (c) 2018 International journal of life sciences
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Articles published in the International Journal of Life Sciences (IJLS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJLS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJLS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJLS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.