Prevalence of Extended-Spectrum of Beta-Lactamase-producing class a genes in Escherichia coli and Klebsiella pneumoniae isolated from patients urine samples in United Arab Emirates

Aishwarya Naira; Banasin Majeed; Hadija Begum; Nabila Rukshana; Nazeerullah Rahamathullah; Zahra Arshad Khan; Anusha Kochath Gobi

doi:10.53730/ijhs.v6nS5.10081

Authors

Aishwarya Naira Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE
Banasin Majeed Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE
Hadija Begum Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE
Nabila Rukshana Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE
Nazeerullah Rahamathullah Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE
Zahra Arshad Khan Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, UAE
Anusha Kochath Gobi Department of Microbiology, Thumbay Laboratory, Gulf Medical University, Ajman, UAE

Keywords:

ESBL genes, blaCTX-M, blaSHV, blaTEM, E. coli, K. pneumoniae

Abstract

The current study aimed to determine the prevalence of class A genes in the Extended Spectrum of β-Lactamase producing E. coli and K. pneumoniae isolated from Patients Urine Samples in United Arab Emirates. A total of 3457 urine samples were collected from October 2018 to January 2019. ESBL screening and confirmatory test were done using MicroScan WalkAway automated bacterial identification system. The ESBL class A gene primers were used to detect the bla_CTX-M, bla_SHV & bla_TEM. Out of the collected urine samples, 474 were reported positive for urinary tract infection. A total of 130 samples were identified as Gram negative ESBL producing species, Escherichia coli (n=107) and Klebsiella pneumoniae (n=23). Amid these 130 ESBL producers, the class A ESBL genes bla_TEM, bla_CTX-M & bla_SHV were detected in 78 isolates of which 59 were E. coli and 19 K. pneumoniae. In the identified 59 E. coli isolates, the most predominant gene was bla_TEM, followed by bla_CTX-M and bla_SHV,and these three genes were detected together in 02 E. coli. In 10 E. coli and 11 K. pneumoniae two class A genes were detected in combination of bla_CTX-M + bla_SHV, bla_SHV+ bla_TEM and bla_TEM+ bla_CTX-M.

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