Histological study of mice kidneys that infected with Echinococcus granulosus & the effect of treatment with nano mixture

https://doi.org/10.53730/ijhs.v6nS5.10123

Authors

  • Alaa Adnan Aljanabi AlSafwa University College
  • Atyaf Ali Sahib AlSafwa University College
  • Noor Hameed Alkharsan AlSafwa University College
  • Kawther A. M. Al-mussawi Kerbala University

Keywords:

Nano mixture, Echinococcus granulosus, Histological

Abstract

The current study included knowing the effect of Silver-Copper nano mixture Ag-Cu and Silver-Copper/Albendazole hybrid mixture Ag-Cu\Alb., in the kidney tissues of mice infected with hydatid cysts that caused by the parasite Echinococcus granulosus experimentally. After 3 months of infection with one dose per day, then the affected kidneys were dissected and extracted and tissue sections were prepared and stained with eosin and hematoxylin dyes, and this was compared to the animals dosing with the traditional drug of Alb. and with the presence of a positive control (infected without treatment) and negative control (uninfected), The results showed that the traditional drug did not cause any change in the tissue, as well as the concentration of 400 mg / kg of nano mixture, while the concentrations of 800 and 1200 mg / kg of the hybrid mixture had a better effect as the tissue appeared free from vascular congestion, as was the concentration of 1200 mg/kg of the hybrid mixture had a good effect on the tissue, as it did not show vascular congestion compared to the lower concentrations, and when comparing the two mixtures with the control treatments, while the infiltration of inflammatory cells remained....

Downloads

Download data is not yet available.

References

Abdulhameed, M. F. ; Robertson, I. D.; Al-Azizz, S. A.; & Habib, I. (2019). Neglected Zoonoses and the Missing Opportunities for One Health Education: The Case of Cystic Echinococcosis among Surgically Operated Patients in Basrah ; Southern Iraq. Diseases; 7(1): 4.

Abulaihaiti, M. ; Weiwu, X. ; Qiao, L. ; Lang, Lv. H. ; Weizhang, H. ; Aduwayi, N. ; Wang, Y. ; Wang, X. & Peng, X. (2015). Efficacy of albendazole – chitosan microsphere –based treatment for alveolar echinococcosis in mice. PLOS Neglected Tropical Diseases . 9(10) .

Ahmadpour, E. ; Godrati, Z. ; Spotin, A. ; Norouzi, R. ; Hamishehkar, H. ; Nami, S. ; Heydarian, P. ; Rajabi, S. ; Mohammadi, M. & Perez – cordong (2019). Nanostrructure lipid carriers of ivermectin as anovel drug delivery system in hydatidosis . Journal of parasites & vectors. 12(469): 1-9 .

Al-hamiary , A. K. O. (2010). Evaluation of the effectiveness of Datura stramonium seed extract on the growth and development of Echinococcus granulosus in Balb/c albino mice (pharmacological, histological and immunological study). PhD thesis, College of Science, Kufa University , 167 p.

Ali‚ A. A. & Salih‚ N. E. (2000) Histopathological changes in the spleen of mice treated with Pullulan and infected with unilocular hydatid disease . Riv. Parasitol. 17 (61) : 198-202 .

Aljanabi , A. A. A. (2021) . The effectiveness of nano-composite loaded Albendazole in some immunological markers in cystic echinococcosis : in vivo . study . A thesis of doctorate , College of Education for Pure Science , Kerbala University . 175 p.

Asharani, P.V. ; Low Kah Mun, G. ; Hande, M.P. & Valiyaveettil, S. (2009). Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 3(2): 279-290.

Athmar, K. A. A. & Ban-Abbas, A. M. (2014). Immunization mice with DNA from protoscolices of human hydatid cyst. Immunological study. International Journal Advaced Biology Research . 4(1): 89-95.

Bancroft, J. & Stevens, A. (1982). Theory and practice of histological technique . 2nd edn. ; Churchill Livingstone. Edinburgh and London.

Center for Food Security and Public Health (CFSPH) . . (2020) Echinococcosis . page 1-14 http://www.cfsph.iastate.edu/

Dumitru, I.M.(2019). Medical Treatment of Cystic Echinococcosis. Licensee IntechOpen . 82 p.

Huh, A. J. & Kwon, Y. J. (2011) . Nanoantibiotics . A new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era . Journal of Controlled Release . 156 : 128–145 .

Khan, I. ; Saeed, K. & Khan, I. (2017) . Nanoparticles: Properties; applications and toxicities. Arabian Journal of Chemistry. 12(7) : 908-931.

Liang, W. ; Wang, X.C. ; Zhang, S.J. ; Sun, H. ; Max, X. & Peng, X.Y. (2014). Efficacy of albendazole–chitosan microspheres against Echinococcus granulosus infection in mice. Journal of Parasitology & Parasitic Diseases . 32 (3) : 188-192.

Mentes, A. ; Yalaz, S. ; Killi, R. & Altintas, N. (2000). Radical treatment for hepatic Echinococcosis. HPB. 2(1): 49-54.

Rahimi, M.T. ; Ahmadpour, E. ; Esboei, R. B. ; Spotin, A. ; Koshki, M.H.K. ; Alizadeh, A.; Honary, S.; Barabadi, H. ; Mohammadig, M. A. (2015) . Scolicidal activity of biosynthesized silver nanoparticles against Echinococcus granulosus protoscolices. International Journal of Surgery. 19:128-133.

Retchkiman-Schabes, P.S. ; Canizal, G. ; Becerra-Herrera, R. ; Zorrilla, C. ; Liu, H.B. & Ascensio, J.A. (2006) . Biosynthesis and characterization of Ti/Ni bimetallic nanoparticles. Optical Materials . 29(1): 95-99.

San,Y. ; Chen, D. ; Pan, Y. ; Qu, W. ; Hao, H. ; Wang, X. ; Liu, Z. & Xie, S. (2019). Nanoparticles for antiparasitic drug delivery . Iranian Journal of drug delivery. 26(1): 1206 – 1221.

Smyth, J. D. (1987). Changing concepts in the microecology, macroecology and epidemiology of hydatid disease. In : Helminth Zoonose. Geerts, S. , Kumar, V. and Brandt, J. (Ed. ) Martinus Nyhoff Publishers.

Fitra, Legowo, D., Utomo, B., Suroto, N. S., Parenrengi, M. A., & Al-Fauzi, A. (2021). Intracranial foreign body granuloma caused by oxidized cellulose polymer and etherified sodium carboxymethyl cellulose: an experimental study with orictolagus cuniculus rabbits. International Journal of Health & Medical Sciences, 4(2), 267-272. https://doi.org/10.31295/ijhms.v4n2.1741

Thys, S. ; Sahibi, H.; Gabriël, S.; Rahali, T.; Lefèvre, P.; Rhalem, A. & Dorny, P. (2019). Community perception and knowledge of cystic echinococcosis in the high atlas mountains , Morocco . BMC public health. 19(1): 118.

Vuitton, D.A. (2003) . The ambiguous role of immunity in echinococcosis: protection of the host or of the parasite? Acta Tropica . 85(2): 119-32.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Wangoo, A. ; Ganguly, N. & Mahjan, R.C. (1989) . Phagocytic function of monocytes in murine model of Echinococcus granulosus of human origin . Indian Journal Of Medical Research . 89: 40-42.

Zheng, J.; Wu, X. ; Wang, M. ; Ran, D. ; Xu, W.& Yang, J. (2008). Study on the interaction between silver nanoparticles and nucleic acids in the presence of cetyltrimethyl ammonium bromide and is analytical application. Talanta . 74 (4): 526-532.

Published

01-07-2022

How to Cite

Aljanabi, A. A., Sahib, A. A., Alkharsan, N. H., & Al-mussawi, K. A. M. (2022). Histological study of mice kidneys that infected with Echinococcus granulosus & the effect of treatment with nano mixture. International Journal of Health Sciences, 6(S5), 6365–6375. https://doi.org/10.53730/ijhs.v6nS5.10123

Issue

Section

Peer Review Articles