Phytochemical, antioxidant and cytotoxic potential of punica granatum extracts on leukemia cell line
Keywords:
Pomegranate fruit, DNA damage, leukemia, phenol, MTT assay, HCS methodAbstract
Pomegranate fruit is edible and has been used on leukemia cell lines and protecting DNA damage known antioxidant efficacy the aim of the current work is to explore the potential benefit of pomegranate (Punica granatum) extract as antioxidant and cytotoxic to leukemia cell line. Methanol was used to extract pomegranate fruit so that to test its antioxidant and cytotoxicity on two cell lines (Hepatic cell line WRL68 and Leukemia cell line HL60). This assay was classified as determining antioxidant effect using 3-(4,5-dimethylthiazol-2yl) 2,5diphenyl tetrazolium (MTT) assay on two cell lines (Hepatic cell line WRL68 and Leukemia cell line HL60) for different concentrations (25, 50, 100, 200 and 400 µg mL-1). These examine the efficacy of the phenol extract on HL60 cell apoptosis, morphology, viability of cells, total nuclear intensity, cell membrane potential, mitochondrial membrane potential, and cytochrome C release were evaluated by High Content Screening (HCS) assay. The current results revealed that the methanol extract of pomegranate fruit had the strongest antioxidant activity, which increased when concentrations were raised (28.27 and 61.57%). The results demonstrated that phenol can inhibit HL60 cell line from growing, with increased concentrations. The inhibition become stronger at 400 µg mL-1 of phenol extract.
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