Detection of eimeria species in domestic goat with both microscopic and molecular methods in Al-Diwaniyah Province
Keywords:
PCR, eimeria, 18S rRNAAbstract
The aim of this study which conducted in Al-Diwaniyah province during the period from October (2021) to April (2022) was to determine the prevalence of Eimeria infection in goat and study the effect of some epidemiological factor such as sex ,age and months on the infection rates addition to that and molecular identification of Eimeria. Two hundred and ten fecal sample were collected, from goat in four different regions in Al-Diwaniyah province included (Al-Shamiya, Al-Sunyih, Ghamas and Al-Mohanawih) at one visit per a week for each regions. The results of microscopic examination showed that 169(80.48%) of goat were infected. According to morphological study, eight Eimeria species were recorded which include E. arloingi, E. alijevi, E. ninakohlyakimovae, E. christenseni, E. jolchijevi, E. hirci, E. caprovina and E. caprina. The results showed that, Eimeria arloingi were recorded at highest rate (22.86%), while E. caprina was observed at lowest rate (3.33%). Significant (P<0.01) differences was found between prevalence of infections according to Eimeria species. Out of 120 fecal sample were collected from goat female and 90 fecal samples were collected from male which examined as Eimeria species, the results showed that, 99(82.5%) females and 70(77.78%) males were confirmed to be infected.
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