Effect of TNF-α gene polymorphism on fertility insufficiency in Holstein cows
Keywords:
TNF-α gene, polymorphism, Holstein cowsAbstract
The current study was conducted in private station located in the middle of Iraq by using 100 blood samples from 100 Holstein cows to isolate DNA and determine the genotypes of TNF-α gene in tow regions on reproductive performance. Results showed two alleles with co-dominance and three genotypes was detected in 1st position (4th exon), the allele frequency was 0.695 and 0.305 for A and C allele respectively. The allele frequency in 2nd region (Flank region) was 0.64 and 0.36 for A and G allele respectively. Results showed a significant effect of gene polymorphism in 4th exon on retained placenta rate which differed significantly (P≤0.01) among cow groups, the highest rate was recorded in cows with cows with AC genotype about 0.24. A significant effect of gene polymorphism in 4th exon on ovulation weakness rate, the highest rate was recorded in cows with cows with CC genotype about 0.12. Parturition difficulties rate differed significantly (P≤0.01) , the highest rate was recorded in cows with mutant genotype namely,0.18. Results showed a significant effect of gene polymorphism in flank region, retained placenta rate was differed significantly (P≤0.01) among cow groups, the highest rate was recorded in cows with GC genotype about 0.20.
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Bojarojć-Nosowicz; E. Kaczmarczyk; A. Stachura and M. Kubińska. 2015. Tumor necrosis factor-alpha (TNFα) gene polymorphism and expression of membrane-bound TNFα protein on CD11b+ and IgM+ cells in cows naturally infected with bovine leukemia virus. Pol J Vet Sci. 2015;18(3): 533-9. doi: 10.1515/ pjvs-0069.
Crowe, M. A; M. Hostens and Geert O. 2018. Reproductive management in dairy cows the future. Irish Vet. J. DOI 10.1186/s13620- 017-0112-y.
Getahun, K.; D. Hunde; M. Tadesse and Tadesse, Y. 2019. Reproductive performance crossbreed dairy cattle at Holetta Agriculture Research Center. Livestock Research for Rural Development. 1-10.
Kawasaki Y.; Y. Aoki; F. Magata; A. Miyamoto; C. Kawashima; T. Hojo; K. Okuda; K. Shirasuna and T. Shimizu. 2014. The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle. J Reprod Dev. 60(3):173-8.
Lendez, P. A.; M.Cuestaa; M. Victoria; N. Fariasa; A. Alejandro; V. Marcelo; D. Ghezzib; D. Mota-Rojasd ; G. Laura; D. Maríaand C. Ceriania. 2021. Alterations in TNF-α and its receptors expression in cows undergoing heat stress. https:// doi.org/ 10.1016/j. vetimm. 110232Get rights and content.
Mao L. ; L. Zong, Y. Wang; J. Zeng; Y. gui Fu; Xin Zhao, Xing-qiang Rao, Yu-xin Huang, Zheng-mei Xu, Xiao-nan Zhu.2012. Association of the tumor necrosis factor-alpha -1031T/C and its combination with interleukin-6 -634C/G gene polymorphisms with susceptibility to endometriosis. 47(5):328-32.
Sakamoto, N .2015. Phase I clinical trial of antilogous NK cell therapy using novel expansion method in patients with advanced digestive cancer. J. Transl. Med, 13:277.
Sambrook, J. and D. Russell.2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor. New York. USA.
Santhia, K., & Sudiasa, W. (2019). Human rabies epidemiology in Bali, Indonesia. International Journal of Health & Medical Sciences, 2(1), 7-16. https://doi.org/10.31295/ijhms.v2n1.77
SAS. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9. 1th ed. SAS. Inst. Inc. Cary. N.C. USA.
Silva, J.R.; Lima, F.E.; A.L. Souza, and Silva, A.W. 2020. Interleukin-1β and TNF-α systems in ovarian follicles and their roles during follicular development, Oocyte maturation and ovulation. Zygote (Cambridge, England)., 1.
Walsh S. W. ; E. J. Williams and Evans A.C.O. 2018.A review of the causes of poor fertility in high milk producing dairy cows. Animal Reproduction Science. Volume 123, Issues 3–4, 127-138.
Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202
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