Evaluation of the synergistic effect of cinnamon and probiotic filtrate against UTI
Keywords:
cinnamon, UTI, bacteriaAbstract
This study was designed to evaluate the effects of cinnamon and probiotics both alone and in combination against pathogenic bacteria isolated from UTI patients. One hundred and ten male and female patients with a urinary tract infection (UTI) who visited private health care facilities were asked to provide urine samples. The urine samples were inoculated on Blood and MacConkey agars and incubated for 24 hrs. at 37 degrees Celsius. After 24 hours of incubation, no development was seen in the cultures, thus they were kept in the incubator for a further 48 hours. Gram staining was used to classify bacteria, and then conventional microbiological culture and biochemical properties were used to identify the isolates. Cinnamomum bark oil was extracted manually. Mueller Hinton (MH) agar plates were infected with 108 CFU/ml suspensions of microorganisms; this method was used to determine the agar disc diffusion test. Discs of sterile Whatman paper No. 3 impregnated with 10 g of the compounds were placed on top of MH agar that had been infected with bacteria. For 18 hours, the plates were kept at 37°C. Inhibition zones against the various bacterial strains were used to evaluate the antibacterial impact.
Downloads
References
A. Berthold-Pluta, L. Stasiak-Różańska, A. Pluta, M. Garbowska, Antibacterial activities of plant-derived compounds and essential oils against Cronobacter strains, European. Eur. Food. Res. Technol. 245(5) (2019) 1137-1147.
A. Intorasoot, P. Chornchoem, S. Sookkhee, S. Intorasoot, Bactericidal activity of herbal volatile oil extracts against multidrug-resistant Acinetobacter baumannii, J. Intercult. Ethnopharmacol. 6(2) (2017) 218-222.
A. Pooja, N. Arun, K. Maninder, GC-MS profile of volatile oils of Cinnamomum Zeylanicum Blume and Ocimum kilimandscharicum Baker ex Gurke, Int. J. Pharm. Sci. Rev. Res. 19(2) (2013) 124-126.
A. Osuntoki, I. Korie Antioxidant activity of Whey from milk fermented with lactobacillus species isolated from Nigerian fermented foods Food Technol Biotechnol, 48 (4) (2010), pp. 505-511.
A.C. Murry, A. Hinton, H. Morrison. Inhibition of growth of Escherichia coli, Salmonella typhimurium and Clostridium perfringens on chicken feed media by Lactobacillus salivarius and Lactobacillus plantarum Int J Poult Sci, 3 (2004), pp. 603-607.
A.D. Melo, A.F. Amaral, G. Schaefer, F.B. Luciano, C. de Andrade, L.B. Costa, M.H. Rostagno Antimicrobial effect against different bacterial strains and bacterial adaptation to essential oils used as feed additives Can. J. Vet. Res., 79 (4) (2015), pp. 285-289
Abdulrahman I. Antimicrobial Susceptibility Pattern of Pathogenic Bacteria Causing Urinary Tract Infections at Azadi Hospital In Duhok CityKurdistan Region of Iraq. Science Journal of University of Zakho. 2018;6. doi: 10.25271/2018.6.2.435.
Al-Naqshbandi AA, Chawsheen MA, Abdulqader HH. Prevalence and antimicrobial susceptibility of bacterial pathogens isolated from urine specimens received in rizgary hospital - Erbil. J Infect Public Health. 2019;12(3):330-6.
Ashraf M, Arshad M, Siddique M and Muhammad G. 2009. In Vitro Screening of Locally Isolated Lactobacillus species for Probiotic Properties. Pakistan Vet. J., 29(4): 186-190.
Assafi M, Ibrahim N, Hussein N, Taha A, Balatay A. Urinary Bacterial Profile and Antibiotic Susceptibility Pattern among Patients with Urinary Tract Infection in Duhok City, Kurdistan Region, Iraq. Int. J. Pure Appl. Sci. Technol. 2015;30:54-63.
Ayelign B, Abebe B, Shibeshi A, Meshesha S, Shibabaw T, Addis Z, et al. Bacterial isolates and their antimicrobial susceptibility patterns among pediatric patients with urinary tract infections. Turk J Urol. 2018;44(1):62-9.
B. Kaskatepe, M.E. Kiymaci, S. Suzuk, S.A. Erdem, S. Cesur, S. Yildiz, Antibacterial effects of cinnamon oil against carbapenem resistant nosocomial Acinetobacter baumannii and Pseudomonas aeruginosa isolates,Ind. Crops. Prod. 81 (2016) 191-194.
Cevallos, M. A. S., Rosado, C. A. Z., & Terán, O. V. T. (2019). The procedure used on diagnostic evaluation process. International Journal of Health & Medical Sciences, 3(1), 1-10. https://doi.org/10.31295/ijhms.v3n1.98
Costello RB, Dwyer JT, Saldanha L, Bailey RL, Merkel J, Wambogo E. Do cinnamon supplements have a role in glycemic control in type 2 diabetes? A narrative review. J Acad Nutr Diet. 2016;116(11):1794–1802.
D.F.R.P. Elizete, R.S. Carlos Biochemical characterization and identification of probiotic Lactobacillus for swine, vol. 23, B.CEPPA, Curitiba (2005), pp. 299-310.
De Francesco MA, Ravizzola G, Peroni L, Negrini R, Manca N. Urinary tract infections in Brescia, Italy: etiology of uropathogens and antimicrobial resistance of common uropathogens. Med Sci Monit. 2007;13(6):Br136-44. [PubMed: 17534228].
Emiru T, Beyene G, Tsegaye W, Melaku S. Associated risk factors of urinary tract infection among pregnant women at Felege Hiwot Referral Hospital, Bahir Dar, North West Ethiopia. BMC Res Notes. 2013;6:292. doi: 10.1186/1756-0500-6-292. [PubMed: 23885968]. [PubMed Central: PMCPmc3750516].
Emiru T, Beyene G, Tsegaye W, Melaku S. Associated risk factors of urinary tract infection among pregnant women at Felege Hiwot Referral Hospital, Bahir Dar, North West Ethiopia. BMC Res Notes. 2013;6:292.
F. Guarner, A.G. Khan, J. Garisch, R. Eliakim, A. Gangl, A. Thomson, et al. World gastroenterology organisation guidelines: probiotics and prebiotics J Clin Gasreoenterol, 46 (6) (2012), pp. 468-481.
Foxman B. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Am J Med. 2002;113 Suppl 1A:5s-13s. doi: 10.1016/s0002-9343(02)01054-9. [PubMed: 12113866].
G.K. Jayaprakasha, L.J.M. Rao Chemistry, biogenesis, and biological activities of Cinnamomum zeylanicum Crit. Rev. Food Sci. Nutr., 51 (2011), pp. 547-562
Gallaher, D., Khil, J. (1999) The effect of synbiotics on colon carcinogenesis in rats. J Nutr 129 (7): 1483-1487.
Gharaei-Fathabad, E.G. and M. Eslamifar, 2011. Isolation and applications of one strain of lactobacillus paraplantarum from tea leaves (Camellia sinensis). Am. J. Food Technol., 6: 429-434.
Gomes AC, Bueno AA, de Souza RGM, Mota JF. Gut microbiota, probiotics and diabetes. Nutr J. 2014;13(1):60.
H.Y. Cui, et al., Antibacterial activity and mechanism of cinnamon essential oil and its application in milk, J. Anim. Plant. Sci. 26(2) (2016) 532-541.
J. Sfeir, C. Lefrançois, D. Baudoux, S. Derbré, P. Licznar, In vitro antibacterial activity of essential oils against Streptococcus pyogenes, Evid. Based. Com. Alt. Med. 2013 (2013) 1-10.
J.O. Kong, S.M. Lee, Y.S. Moon, S.G. Lee, Y.J. Ahn Nematicidal activity of cassia and cinnamon oil compounds and related compounds toward Bursaphelenchus xylophilus (Nematoda: parasitaphelenchidae) J. Nematol., 39 (1) (2007), pp. 31-36
Khoshbakht R, Salimi A, Shirzad Aski H, Keshavarzi H. Antibiotic Susceptibility of Bacterial Strains Isolated From Urinary Tract Infections in Karaj, Iran. Jundishapur J Microbiol. 2012;6(1):86-90. doi: 10.5812/jjm.4830.
Kobyliak N, Falalyeyeva T, Mykhalchyshyn G, Kyriienko D, Komissarenko I. Effect of alive probiotic on insulin resistance in type 2 diabetes patients: randomized clinical trial. Diabetes Metab Syndr Clin Res Rev. 2018;12(5):617–624.
Laupland KB, Ross T, Pitout JD, Church DL, Gregson DB. Community-onset urinary tract infections: a population-based assessment. Infection. 2007;35(3):150-3.
Linhares I, Raposo T, Rodrigues A, Almeida A. Frequency and antimicrobial resistance patterns of bacteria implicated in community urinary tract infections: a ten-year surveillance study (2000-2009). BMC Infectious Diseases. 2013;13(1):19. doi: 10.1186/1471-2334-13-19.
M. Raeisi, H. Tajik, A. Yarahmadi, and S. Sanginabadi, Antimicrobial effect of cinnamon essential oil against Escherichia Coli and Staphylococcus aureus, Health. Scope. 4(4) (2015) e21808.
Magliano E, Grazioli V, Deflorio L, Leuci AI, Mattina R, Romano P, et al. Gender and age-dependent etiology of community-acquired urinary tract infections. ScientificWorldJournal. 2012;2012:349597. doi: 10.1100/2012/349597. [PubMed: 22629135]. [PubMed Central: PMCPmc3351074].
Maierean SM, Serban M-C, Sahebkar A, Ursoniu S, Serban A, Penson P, et al. The effects of cinnamon supplementation on blood lipid concentrations: a systematic review and meta-analysis. J Clin Lipidol. 2017;11(6):1393–1406.
Mansour A, Mahdinezhad M, Pourdangchi Z. Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics. Jundishapur Journal of Microbiology. 2009;2(3):118-23.
Markey, B., Leonard, F., Archambault, M., Cullinane, A., & Maguire, D. (2013). Clinical veterinary microbiology e-book. Elsevier Health Sciences.
Mirmiran P, Bahadoran Z, Azizi F. Functional foods-based diet as a novel dietary approach for management of type 2 diabetes and its complications: a review. World J Diabetes. 2014;5(3):267.
Mirsoleymani SR, Salimi M, Shareghi Brojeni M, Ranjbar M, Mehtarpoor M. Bacterial pathogens and antimicrobial resistance patterns in pediatric urinary tract infections: a four-year surveillance study (2009-2012). Int J Pediatr. 2014;2014:126142.
P.V. Rao, S.H. Gan Cinnamon: a multifaceted medicinal plant Evid. Based Complement. Alternat. Med., 2014 (2014), p. 642942
Ranjbar R, Haghi-Ashtiani MT, Jafari N, Abedini M. The Prevalence and Antimicrobial Susceptibility of Bacterial Uropathogens Isolated from Pediatric Patients. Iranian Journal of Public Health. 2009;38.
Ronald A. The etiology of urinary tract infection: Traditional and emerging pathogens. Disease-a-Month. 2003;49(2):71-82. doi: 10.1067/mda.2003.8.
Ronald AR, Nicolle LE, Stamm E, Krieger J, Warren J, Schaeffer A, et al. Urinary tract infection in adults: research priorities and strategies. Int J Antimicrob Agents. 2001;17(4):343-8. doi: 10.1016/s0924-8579(01)00303-x. [PubMed: 11295419].
S. Mathew, T.E. Abraham Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models Food Chem., 94 (4) (2006), pp. 520-528
S. Shreaz, W.A. Wani, J.M. Behbehani, V. Raja, M. Irshad, M. Karched, I. Ali, W.A. Siddiqi, L.T. Hun Cinnamaldehyde and its constituents, a novel class of antifungal agents Fitoterapia, 12 (2016), pp. 116-131
S.J. Koppikar, A.S. Choudhari, S.A. Suryavanshi, S. Kumari, S. Chattopadhyay, R. Kaul-Ghanekar Aqueous cinnamon rxtract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential BMC Canc., 10 (1) (2010), p. 210.
S.S. Cheng, J.Y. Liu, C.G. Huang, Y.R. Hsui, W.J. Chen, S.T. Chang Insecticidal activities of leaf essential oils from Cinnamomum osmophloeum against three mosquito species Bioresour. Technol., 100 (1) (2009), pp. 457-464
S.Y. Wang, P.F. Chen, S.T. Chang Antifungal activities of essential oils and their constituents from indigenous cinnamon (Cinnamomum osmophloeum) leaves against wood decay fungi Bioresour. Technol., 96 (7) (2005), pp. 813-818
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Weekes LM. Antibiotic resistance changing management of urinary tract infections in aged care. Med J Aust. 2015;203(9):352.
Y. Zhang, X. Liu, Y. Wang, P. Jiang, S.Y. Quek, Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus, Food. Control. 59 (2016) 282-289.
Y.T. Tung, M.T. Chua, S.Y. Wang, S.T. Chang Antiinflammation activities of essential oil and its constituents from indigenous cinnamon (Cinnamomum osmophloeum) twigs Bioresour. Technol., 99 (9) (2008), pp. 3908-3913
Y.T. Tung, P.L. Yen, C.Y. Lin, S.T. Chang Antiinflammatory activities of essential oils and their constituents from different provenances of indigenous cinnamon (Cinnamomum osmophloeum) leaves Pharm. Biol., 48 (10) (2010), pp. 1130-1136
Z. Lu, Q. Jia, R. Wang, X. Wu, Y. Wu, C. Huang, Y. Li Hypoglycemic acivities of A and B-type procyanidin oligomer-rich extracts from different Cinnamon barks Phytomedicine, 18 (4) (2011), pp. 298-302
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
