Subtypes of Cryptosporidium parvum in human in Al-Diwaniyah province, Iraq
Keywords:
cryptosporidium parvum, nested-PCR, sequences, humanAbstract
The current study was designed to diagnose the species of Cryptosporidium parasites in humans and to identify the subtypes of C .parvum by using the Hsp70 gene. In Al-Diwaniyah province it was conducted during the period from September 2021 to the end of February 2022. 100 stool samples were taken from individuals who differed in sex, age and place of residence. After extracting the genomic DNA of the parasite, the results of the PCR test showed that the infection rate of the parasite reached 30%. Then, N-PCR was performed on the same samples in which Cryptosporidium DNA was identified, targeting the 18SrRNA gene. Two species of Cryptosporidium identified and recorded in the NCBI-Genbank database are C.parvum:6/10 , C.hominis 4/10.While the result of Subtyping of C.parvum by using Hsp70 gene was Subtype_1 40% (2/5)and Subtype_2 60%(3/5). The identification and characterization of Cryptosporidium spp in humans was very important to avoid infection for people and healers and to implement control programs.
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