Isolation and molecular identification of pathogenic cryptococcus neoformans from pigeon dropping and human sputum
Keywords:
isolation, molecular identification, pathogenic cryptococcus neoformans, pigeon dropping, human sputumAbstract
This study is designed to isolate and molecular identification of C. neoformans, C. neoformans is pathogenic yeast and effect immunocompromised and immunocompetent. Methods: collect 50 samples from pigeon dropping and 50 samples from pigeon fanciers (sputum). The collection time was extended from November 2021 to February 2022, then culture at SDA, BSA, Cryptococcus Differential agar, esculin agar, Eucalyptus leaves agar media and Brain heart infusion agar with methyldopa, biochemical test including urease test and methyldopa, and then confirm identification by molecular identification by PCR technique sequencing and genetic analysis. The results showed that 3 swaps taken from sputum of human included cryptococcus neoformans and 6 swaps from bird dropping included cryptococcus neoformans. This study indicated that the virulence and normal habitat of cryptococcus neoformans. These results indicate that there may be a risk for human exposure to cryptococcal disease due to inhalation of basidiospores from dropping of pigeon in pigeon houses
Downloads
References
Almeida, F., Wolf, J. M., & Casadevall, A. (2015). Virulence-associated enzymes of Cryptococcus neoformans. Eukaryotic cell, 14(12), 1173-1185.
Archana, .-., Datta, C., & Tiwari, P. (2016). Impact of environmental degradation on human health. International Research Journal of Management, IT and Social Sciences, 3(1), 1-6. Retrieved from https://sloap.org/journals/index.php/irjmis/article/view/341
Baron, E.J. and Finegold, S.M. (1990). "Diagnostic Microbiology Laboratory Methods in Basic Mycology" 8th Ed. C.V. Mosby Company. Pp: 748775.
Chaskes, S., Frases, S., Cammer, M., Gerfen, G., & Casadevall, A. (2008). Growth and pigment production on D-tryptophan medium by Cryptococcus gattii, Cryptococcus neoformans, and Candida albicans. Journal of clinical microbiology, 46(1), 255-264.
Cogliati, M. (2013). Global molecular epidemiology of Cryptococcus neoformans and Cryptococcus gattii: an atlas of the molecular types. Scientifica, 2013.
Elhariri, M., Hamza, D., Elhelw, R., & Refai, M. (2016). Eucalyptus tree: a potential source of Cryptococcus neoformans in Egyptian environment. International journal of microbiology, 2016.
Karkowska-Kuleta, J., Rapala-Kozik, M., & Kozik, A. (2009). Fungi pathogenic to humans: molecular bases of virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. Acta Biochimica Polonica, 56(2).
Khanal, B., Sharma, S. K., & Deb, M. (2002). Cryptococcal meningitis in a non-AIDS patient. Journal of Nepal Medical Association, 41, 323-325.
Lau, A., Chen, S., Sorrell, T., Carter, D., Malik, R., Martin, P., & Halliday, C. (2007). Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. Journal of clinical microbiology, 45(2), 380-385.
Li, A., & Wu, S. (1992). An urease negative Cryptococcus neoformans. Wei Sheng wu xue bao= Acta Microbiologica Sinica, 32(1), 68-71.
Lusia Leal, A., Faganello, J., Cristina Bassanesi, M., & Vainstein, M. H. (2008). Cryptococcus species identification by multiplex PCR. Medical Mycology, 46(4), 377-383.
Menezes, R. D. P., Penatti, M. P. A., & Pedroso, R. D. S. (2011). Different culture media containing methyldopa for melanin production by Cryptococcus species. Revista da Sociedade Brasileira de Medicina Tropical, 44, 591-594.
Mitchell, T. G., Freedman, E. Z., White, T. J., & Taylor, J. W. (1994). Unique oligonucleotide primers in PCR for identification of Cryptococcus neoformans. Journal of Clinical Microbiology, 32(1), 253-255.
Moťková, P., & Vytřasová, J. (2012). Comparison of methods for isolating fungal DNA. Czech Journal of Food Sciences, 29(Special Issue), S76-S85.
Okagaki, L. H., Strain, A. K., Nielsen, J. N., Charlier, C., Baltes, N. J., Chrétien, F., ... & Nielsen, K. (2010). Cryptococcal cell morphology affects host cell interactions and pathogenicity. PLoS pathogens, 6(6), e1000953.
Pham, C. D., Ahn, S., Turner, L. A., Wohrle, R., & Lockhart, S. R. (2014). Development and validation of benomyl birdseed agar for the isolation of Cryptococcus neoformans and Cryptococcus gattii from environmental samples. Sabouraudia, 52(4), 417-421.
Sidrim, J. J. C., Costa, A. K. F., Cordeiro, R. A., Brilhante, R. S. N., Moura, F. E. A., Castelo-Branco, D. S. C. M., ... & Rocha, M. F. G. (2010). Molecular methods for the diagnosis and characterization of Cryptococcus: a review. Canadian journal of microbiology, 56(6), 445-458.
Staib, F., Seibold, M., Antweiler, E., Fröhlich, B., Weber, S., & Blisse, A. (1987). The brown colour effect (BCE) of Cryptococcus neoformans in the diagnosis, control and epidemiology of C. neoformans infections in AIDS patients. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, 266(1-2), 167-177.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938
Tendulkar, S. R., Gupta, A., & Chattoo, B. B. (2003). A simple protocol for isolation of fungal DNA. Biotechnology letters, 25(22), 1941-1944.
Zarrin, M., Jorfi, M., Amirrajab, N., & Rostami, M. (2010). Isolation of Cryptococcus neoformans from pigeon droppings in Ahwaz, Iran. Turkish Journal Of Medical Sciences, 40(2), 313-316.
Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational biology, 7(1-2), 203-214.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
