Molecular study of epstein Barr virus among patients with malignant diseases in al Najaf and Diwaniyah governorates
Keywords:
Epstein-Barr virus, cancer, NCBI-BLAST, ymphotropiAbstract
The present study is an effort to study the Epstein-Barr virus and it is relationship to the different types of cancers and their relationship to the virus and to the demographic distribution of the patients. our study started from Sep 2019 to Dec 2020 , blood samples were collected (2 ml of venous blood) were drawing by disposable syringe under a septic conditions. From patients with age grouped more 13 years old , represented by 47 specimens that subjected to molecular examination in Nested PCR technique detecting EBV LMP-1 in patients' blood as a main route. The study has included patients with different types of cancers from different areas. 47 specimens submitted to molecular examination, polymerase chain reaction (PCR) and nested PCR. Where EBV LMP-1 detected in 20 positive Specimens. All the virus isolates were sent to NCBI-BLAST to confirm the variation of the isolates. The study summarize that there is a strong relationship between EBV and some cancer diseases and show no significant difference according to age or gender or hereditary.
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