Role of nitric oxide in detection toxoplasmosis patients in Al-Najaf Province
Keywords:
Genotyping, Toxoplasma gondii, B1gene, Relation, Nitric OxideAbstract
This study was aimed to estimate the prevalence of Toxoplasma gondii infection and associated risk factors among patients in Al-Najaf Province and to investigate whether there is a relationship between this infection and one of oxidative stress marker level (nitric oxide (NO). A total of 117 blood samples were collected from Iraqi women with toxoplasmosis at Al-Zahra Teaching Hospital for Maternity and Children in AL-Najaf province. The PCR test was carried out in the Advanced Research Laboratory of the Department of Laboratory Investigations / Faculty of Science – University of Kufa. The estimation of NO marker level in patients and 30 healthy women (control group) was conducted in Al-Ameen Center for Research and Advanced Biotechnology/ Imam Ali Holy Shrine. The results of the PCR (to confirm the toxoplasmosis infection) had shown that 53(45.30%) patients were found positive and 64 (54.70%) were negative. The results of biochemical study revealed that the concentrations of NO level were increased in toxoplasmosis patients compared with the controls. The results of this study highlight on the wide spread of Toxoplasmosis in Iraq, especially in Najaf Governorate. The use of molecular methods to diagnose toxoplasmosis remains the most accurate technique for diagnosing infection.
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