Phenotypic and molecular diagnosis using polymerase chain reaction (PCR) technology for some aflatoxin-producing fungi isolated from food
Keywords:
PCR, Phenotypic, aflatoxinAbstract
The study was conducted in the laboratories of the College of Education for Pure Sciences for the academic year 2021-2022 on fungi that produce aflatoxin toxin that were isolated from some foodstuffs traded in the local markets in the city of Karbala and were diagnosed molecularly using a technique Polymerase chain reaction (PCR) which successfully doubled NS8 and NS1 with prefixes (SSU-) The same primers were used to determine the sequence of nitrogenous bases of rRNA belonging to the International Center for Information Technology Genbank. National Center of Biotechnology Information (NCBI) it has been deposited in a database (fungi biogenic). The results of isolation, which were collected from imported and local foodstuffs, were contaminated with fungi, and that all isolated fungi were producing aflatoxin toxin. This was inferred by using coconut medium with ammonia solution. hf ifheehehai eifenhihehidnhf eht dna Penicillium oxalicum, Penicillium expansum, Aspergillus flavus, Cladosporium Uredinicola, Aspergillus sydowii, Aspergillus Oryzae, Aspergillus Tamarii, Aspergillus nomius, Alternaria triticina, Penicillium griseofulvum, Aspergillus austwicki, Aspergillus flavus, Cladosporium cladosporioides, Actinomucor elegans, Penicillium brevicompatum, Trichophyton mentagrophytes, Gibberella intermedia, Debaryomyces hansenii, Aspergillus caespitosus, Aspergillus versicolor.
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