Immunological evaluation of IL-10 concentration level in sera of digestive disorders patients infected with Blastocystis hominis infection in Al-Najaf city
Keywords:
interleukin-10, Blastocystis hominis, digestive disordersAbstract
Objectives: To evaluating the concentration level of IL-10 in serum of patients suffering from digestive disorders Blastocystis parasitic infection and compared with control group by using ELISA. Methadology: Case-control study design was conducted on 70 patients (33 males and 37 females) at Al-Sadr medical city and Al-Hakim General Hospital and Al-Sajad Hospital. A control group of 70 participitant (29 males and 41 females) who were without any history of disease were included. Their ages ranged between (6 - ≥ 60) years, during the period from the beginning of November 2021 to the end of February 2022. Results: The results showed (48.6%) of culture positive for B.hominis. The results of immunoassay showed mean difference ± standard error difference of IL-10 was (5.878 ± 10.462 Pg/ml) with a 95% confidence interval was (14.809-26.565) in intestinal disorder patients with significant difference (P˂0.001). Conclusion: Interleukin-10 concentration levels have a significant effect on the results of Blastocystosis patients suffering from digestive disorders.
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Abdulla, N. B.; Alsadig, A. A.; Abosalif, K. O. and Saad, M.B. (2014). Gastrointestinal parasites among patients with irritable bowel Syndrome (IBS) in Khartoum state, Sud. Med. Lab. J., 2(2): 18-26.
ALDAHHASI, W. T., TOULAH, F. H., & WAKID, M. H. (2020). Evaluation of common microscopic techniques for detection of Blastocystis hominis. Journal of the Egyptian Society of Parasitology, 50(1), 33-40.
Amany, M. E. and Mohamed M. E. (2008). Identification of Blastocystis hominis in Patients with Irritable Bowel Syndrome using Microscopy and Culture Compared to PCR. Parasitologists United Journal (PUJ), 1, (2): 87-92.
Canavan, C., West, J., and Card, T. (2014). The epidemiology of irritable bowel syndrome. Clinical epidemiology, 6, 71.
Chen, J., Zhang, Y., & Deng, Z. (2012). Imbalanced shift of cytokine expression between T helper 1 and T helper 2 (Th1/Th2) in intestinal mucosa of patients with post-infectious irritable bowel syndrome. BMC gastroenterology, 12(1), 1-6.
Darkoh, C., Comer, L., Zewdie, G., Harold, S., Snyder, N., & DuPont, H. L. (2014). Chemotactic chemokines are important in the pathogenesis of irritable bowel syndrome. PloS one, 9(3), e93144.
Eassa, S. M., Ali, H. S., El Masry, S. A., & Abd El-Fattah, A. H. (2016). Blastocystis hominis among immunocompromised and immunocompetent children in Alexandria, Egypt. Ann Clin Lab Res, 4(2), 92-98.
Ip, W. E., Hoshi, N., Shouval, D. S., Snapper, S., & Medzhitov, R. (2017). Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages. Science, 356(6337), 513-519.
Jalallou, N., Iravani, S., Rezaeian, M., Alinaghizade, A., and Mirjalali, H. (2017). Subtypes distribution and frequency of Blastocystis sp. Isolated from diarrheic and Non-diarrheic patients. Iranian journal of parasitology, 12(1), 63.
Mahmood, O. I., & Khudher, T. A. A. (2018). Diagnostic and Epidemiological study of Blastocystis hominis in Samarra city, Iraq. Tikrit journal of pure science, 21(6), 28-31.
Mohamed, S. K., (2016). Prevalence of Blastocystis hominis and other Enteropathogenes and Their Relationship with Irritable Bowel Syndrome in Al-Muthanna Province.
Parkar, U.; Traub, R. J.; Kumar, S.; Mungthin, M.; Vitali, S.; Leelayoova, S.; Morris, K. and Thompson, R. C. (2007). Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitology, 134, 359-67.
Ramli, R. M., Sinrang, A. W., & Aminuddin. (2021). Levels of alpha-1 acid glycoprotein (AGP) in stunting and non stunting tolls age 36-60 months . International Journal of Health & Medical Sciences, 4(1), 145-149. https://doi.org/10.31295/ijhms.v4n1.1666
Ratnawati, I. G. A. A., Suandayani, N. K. T., & Sutapa, G. N. (2019). The linearity of x-ray devices radiation output and its relationship with patient thickness. International Journal of Physical Sciences and Engineering, 3(3), 1–6. https://doi.org/10.29332/ijpse.v3n3.351
Sabat, R., Grütz, G., Warszawska, K., Kirsch, S., Witte, E., Wolk, K., & Geginat, J. (2010). Biology of interleukin-10. Cytokine & growth factor reviews, 21(5), 331-344.
Sayal, R. A., Hameed, S. A., & Faisal, M. M. (2020). Evaluation of IL-5 concentration level in Irritable Bowel Syndrome patients that suffering from Blastocystis infection in Al-Najaf Province. European Journal of Molecular & Clinical Medicine, 7(2), 3807-3817.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864
Vara, E. J., Brokstad, K. A., Hausken, T., & Lied, G. A. (2018). Altered levels of cytokines in patients with irritable bowel syndrome are not correlated with fatigue. International Journal of General Medicine, 11, 285.
Yang, B., Zhou, X., & Lan, C. (2015). Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome. BMC gastroenterology, 15(1), 1-7.
Yousif, J. J., and Hashem, Z. A. K. (2021). Evaluation of the Level of some Immunological Parameters in Patients with Gastrointestinal Disorders Infected with the Blastocystis Hominis Parasite. REVISTA GEINTEC-GESTAO INOVACAO E TECNOLOGIAS, 11(4), 2322-2330.
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