Study the effect of purified cow milk   glutathione on cancer cell growth (HePG2) in vitro

Kifah Saed Doosh

doi:10.53730/ijhs.v6nS4.11238

Authors

Kifah Saed Doosh
Kifah.s@coagri.uobaghdad.edu.iq
Food science department - College of Agriculture engineering science - Baghdad University-Iraq

Keywords:

cow milk whey, glutathione, HePG2, MTT assay, anticancer

Abstract

Glutathione is an important protein in many biological applications as a potential cancer treatment agent .In this study, glutathione was purified from cow milk whey by gelfiltration using Sephadex G-10 column. To know its ability as anticancer agent the study utilized an in vitro evaluation for the cytotoxic effect of the purified whey glutathione on HePG2 cell line at different concentrations and different exposure time of treatment. The purified glutathione concentrations ranging (6.25 to 400) μg/ml for 24,48 and 72 hours. The effect of glutathione was evaluated by employing MTT assay. The results revealed a significant cytotoxic effect at levels (P<0.05) for all concentrations and for all exposure time as compared to untreated control cells, The inhibition rate IR% increased with the increased in glutathione concentration and incubation period. The highest inhibitory growth was shown at concentration ( 400μg/ml) after 72 hrs of exposure time it was 90% .

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