To evaluate the drug resistance pattern in various antibiotics with biofilm-producing capability of Klebsiella pneumoniae

Rachna Rachna; Mohammad Suhaib; Ravindrasingh Rajput; Tarnmit Nahal

doi:10.53730/ijhs.v6nS8.11590

Authors

Rachna Assistant Professor, Department of Medical Laboratory Technology, School of Allied Health Science, Sharda University, Greater Noida, Uttar Pradesh, India
Mohammad Suhaib Assistant Professor, MBBS, MD, Department of Microbiology, School of Medical Sciences and Research, Sharda, University, Greater Noida, UP.
Ravindrasingh Rajput Associate Professor, MBBS, MD, Department of Pathophysiology, American University of Antigua college of medicine, Antigua and Barbuda
Tarnmit Nahal Medical student, Windsor University School of Medicine, Cayon, Saint Kitts, and Nevis

Keywords:

Klebsiella pneumoniae, biofilm producer, broad spectrum antibiotics, narrow spectrum antibiotics, drug resistance

Abstract

Aim: Objective of study was to evaluate the drug resistance pattern in various antibiotics with the biofilm-producing capability of Klebsiella pneumoniae. Methods: Klebsiella pneumoniae was isolated for identification from total of 83 OPD and IPD patients by analysing colony morphology, microscopic examination, and performed biochemical testing. Antibiotics susceptibility Tests and biofilm-producing capacity was done by the Kirby-Bauer disk diffusion method and adherence quantitative assays. Chi-square test was used and P < 0.05 was measured as statistically significant. Results: Out of 83 isolates, 47 (56.6%) were Biofilm Producer and 36 (43.4%) were non-biofilm producers by tissue culture method, and tube method 62.7% and 37.3% respectively. Among biofilm producer and non-biofilm producer isolates, Klebsiella pneumoniae had the most Resistant to Ceftazidime (100%) & (61.0%), followed by Ceftriaxone (56.0%) & (95.7%) respectively. The association between biofilm and antibiotic resistance was checked to be statistically significant for aminoglycosides, fluoroquinolones, Cephalosporin’s, Imipenem, Meropenem, Amoxyclav, and Piperacillin Tazobactam. No resistance was observed against Colistin and Polymyxin B. Conclusion: The strain Klebsiella pneumoniae isolated from different samples showed drug resistance to all broad-spectrum antibiotics whereas there is no drug resistance was found with narrow-spectrum drugs. This study supports the use of narrow-spectrum antibiotics for most of infections.

Downloads

Download data is not yet available.

References

Akter J, Chowdhury AMMA, Al FM. Study on prevalence and antibiotic resistance pattern of Klebsiella isolated from clinical samples in south east region of Bangladesh. American J Drug Discov Dev. 2014;4:73–9.

Al-Ahmad A, Ameen H, Pelz K, Karygianni L, Wittmer A, Anderson AC, Spitzmüller B, Hellwig E. Antibiotic resistance and capacity for biofilm formation of different bacteria isolated from endodontic infections associated with rootfilled teeth. Journal of Endodontics. 2014 Feb 1;40(2):223-30.

Araujo C D, Balestrino D, Roth L, Charbonnel N, Forestier C (2010) Quorum sensing affects biofilm formation through lip polysaccharide synthesis in Klebsiella pneumonia. Research in Microbiology.

Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime‑producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982;37:318‑26.

Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase‑negative staphylococci to plastic tissue culture plates: A quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 1985;22:996‑1006.

Chung PY. The emerging problems of Klebsiella pneumoniae infections: carbapenem resistance and biofilm formation. FEMS microbiology letters. 2016 Oct 1;363(20).

CLSI. Clinical and laboratory standards institute. Document No M100. Performance standards for antimicrobial susceptibility testing: 27th edition. Wayne, USA: CLSI; 2017.

Dharan Rabina Dumaru*, Ratna Baral and Lok Bahadur Shrestha Study of biofilm formation and antibiotic resistance pattern of gram-negative Bacilli among the clinical isolates at BPKIHS, 6 BMC Res Notes (2019) 12:38.

Diniari, N. K. S., & Aryani, L. N. A. (2022). Characteristics and pharmacological treatment options of delirium patients treated at Sanglah Central General Hospital . International Journal of Health & Medical Sciences, 5(1), 37-43. https://doi.org/10.21744/ijhms.v5n1.1835

Fabrega A, Soto SM, Ballesté-Delpierre C, Fernández-Orth D, Jiménez de Anta MT, Vila J. Impact of quinolone-resistance acquisition on biofilm production and fitness in Salmonella enterica. Journal of Antimicrobial Chemotherapy. 2014 Apr 4;69(7):1815-24.

Ghadiri H, Vaez H, Azarkhiavi KR, Rezaee R, Noormohammadi MH, Rahimi AA, Vaez V, Kalantar E. Prevalence and antibiotic susceptibility patterns of extended-spectrum ß-lactamase and metallo-ß-lactamase-producing uropathogenic. Lab Med. 2014;45(4):291–6.

Gunn JS, Bakaletz LO, Wozniak DJ. What's on the outside matters: the role of the extracellular polymeric substance of gram-negative biofilms in evading host immunity and as a target for therapeutic intervention. J Biol Chem. 2016;291(24):12538–46.

Gyaneshwar Tiwari*, D. R. Arora**, B. Mishra***, Vinita Dogra Comparative Evaluation of Methods Used For Detection of Biofilm Production In Multidrug Resistant Bacteria

Hera Nirwati1*, Kian Sinanjung2, Fahrina Fahrunissa3, Fernando Wijaya3, Sarastia Napitupulu3, Vania P. Hati3, Mohamad S. Hakim1, Andreanita Meliala4, Abu T. Aman1 and Titik Nuryastuti1. Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia . BMC Proceedings 2019, 13(Suppl 11):20.

Hera Nirwati1*, Kian Sinanjung2, Fahrina Fahrunissa3, Fernando Wijaya3, Sarastia Napitupulu3, Vania P. Hati3, Mohamad S. Hakim1, Andreanita Meliala4, Abu T. Aman1 and Titik Nuryastuti1 Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia

Heydari S, Eftekhar F. Biofilm formation and β-lactamase production in burn isolates of Pseudomonas aeruginosa. Jundishapur J Microbiol. 2015;8:3. https ://doi.org/10.5812/jjm.15514 .

Hoffman, L.R., D.A. D’Argenio, M.J. MacCoss, Z. Zhang, R.A. Jones, and S.I. Miller. 2005. Aminoglycoside antibiotics induce bacterial biofilm formation. Nature. 436:1171–1175.

Jagessar RC, Alleyne R Antimicrobial (2001) potency of the aqueous extract of leaves of Terminalia catappa. Academic Research International; 1(3):362-371.

Kaplan, J.B. 2011. Antibiotic-induced biofilm formation. Int. J. Artif. Organs. 34:737–751. Total sample 83.

Kaur N, Kaur A, Singh S. Prevalence of ESBL and MBL producing gram negative isolates from various clinical samples in a tertiary care hospital. Int J Curr Microbiol Appl Sci. 2017;6(4):1423–30.

Lavender, H. F., Jagnow, J. R. & Clegg, S. Bioﬁlm formation in vitro and virulence in vivo of mutants of Klebsiella pneumoniae. Infect. Immunity 72, 4888–4890 (2004).

Lee H W, Koh Y M, Kim J, Lee JC, Lee YC, Seol SY (2008) et al. Capacity of multidrugresistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clinical Microbiology and Infection, 14 (1):49-54.

Lee HW, Koh YM, Kim J, Lee JC, Lee YC, Seol SY, Cho DT, Kim J. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clinical microbiology and infection. 2008 Jan;14(1):49.

Meatherall BL, Gregson D, Ross T, Pitout JD, Laupland KB. Incidence, risk factors, and outcomes of Klebsiella pneumoniae bacteremia. Am J Med. 2009;122(9):866–73

Moscoso, M., Garcı ´a, E. & Lo ´pez, R. Pneumococcal bioﬁlms. Int. Microbial. 12, 77–85 (2009).

Osagie RN, Eyaufe AA, Iserhienrhien O, Okodua M, Onuabonah F, Daibo OO. Antibiotic suscepbility profile of Klebsiella pneumoniae isolated from sputum samples amongst hospitalized adults in parts of Edo state, south-south. Niger Merit Res J. 2017;5(8):378–83.

Perez LR. Acinetobacter baumannii displays inverse relationship between meropenem resistance and biofilm production. Journal of Chemotherapy. 2015 Feb 1;27(1):13-6.

Pramodhini S, Niveditha S, Umadevi S, Kumar S, Stephen S. Antiobiotic resistance pattern of biofilm-forming uropathogens isolated from catheterised patients in Pondicherry, India. Australas Med J. 2012;5(7):344–8.

Punia P, Goel N, Asati S, Phogat R, Chaudhary U. Biofilm detection amongst extended spectrum beta lactamase (ESBL) and metallo beta lactamase (MBL) producing clinical isolates of Acinetobacter baumannii. Int J Enchanced Res Med Dent Care. 2016;3:10.

Ramos PP, Picão R C, Almeida LBG, Nicolás MF (2014) Comparative analysis of the complete genome of KPC-2-producing Klebsiella pneumoniae Kp13 reveals remarkable genome plasticity and a wide repertoire of virulence and resistance mechanisms. BMC Genomics; 15(54):1-16.

Shahidul KM, Farahnaaz F, Sunjukta A. Determination of Antibiotic resistance pattern of Biofilm producing Pathogenic Bacteria associated with UTI. Int J Drug Dev Res. 2013;5(4):312–9.

Shrestha LB, Bhattarai NR, Khanal B. Antibiotic resistance and biofilm formation among coagulase-negative staphylococci isolated from clinical samples at a tertiary care hospital of eastern Nepal. Antimicrob Resist Infect Control. 2017;6:89.

Suandayani, N. K. T. ., Sutapa, G. N. ., & Kasmawan, I. G. A. . (2020). Quality control of X-rays with collimator and the beam alignment test tool. International Journal of Physical Sciences and Engineering, 4(3), 7–15. https://doi.org/10.29332/ijpse.v4n3.468

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938

Van Acker H, Van Dijck P, Coenye T (2014) Molecular mechanisms of antimicrobial tolerance and resistance in bacterial and fungal biofilms. Trends Microbiol 22: 326–333..

Vuotto C, Longo F, Balice M, Donelli G, Varaldo P. Antibiotic Resistance Related to Biofilm Formation in Klebsiella pneumoniae. Pathogens. 2014;3:743–58.

Washington CW Jr, Stephen DA, William MJ, Elmer WK, Gray WP, Paul CSGL. Koneman’s colour atlas and textbook of diagnostic microbiology. 6th ed. Philadelphia: Lippincott Williams & Wilkins; 2006.

Yang, D.; Zhang, Z. Biofilm-forming Klebsiella pneumoniae strains have greater likelihood of producing extended-spectrum beta-lactamases. J. Hosp. Infect. 2008, 68, 369–371.

Zheng JX, Lin ZW, Chen C, Chen Z, Lin FJ, Wu Y, Yang SY, Sun X, Yao WM, Li DY, et al. Biofilm formation in Klebsiella pneumoniae bacteremia strains was found to be associated with CC23 and the presence of wcaG. Front Cell Infect Microbiol. 2018;8:21.