Use of Pseudomonas fluorescens in the production of single-cell oil and estimate the oil content of fatty acids
Keywords:
oil, acids fatty, oil production pseudomonas fluorescens, single cell oilAbstract
The study aimed at producing single-cell oil using Pseudomonas fluorescens bacteria and conducting solubility and solubility testing to confirm oil production and estimate the content of oil produced from fatty acids, corn coalts were used as a carbon source and applied optimal conditions forthe production obtained from the previous study, as used amid liquid corn coalts in the production experiment and oil was extracted with the saxolite device (Soxhletlet) ) We obtained the highest oil productivity by 55.47%, the oil produced was fully soluble in chloroform and dissolved to a lesser degree in ethanol and the inability of the oil produced to melt in water, and the oil produced contained the highest percentage of linoleic acid Unsaturated at 69,214%, while stearyk acid was 23.62%, palmetic acid 2.92%, propionic acid 1.6% and butaric acid 0.146%.
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References
Association of Official Analytical Chemists(AOAC). (1990). Official methods of analyses. Washington, DC: Association of Official Analytical Chemists.
Chatzifragkou, A., Fakas, S. M.; Galiotou-Panayotou; M. Komaitis; G. Aggelis, and S. Papanikolaou, (2010). Eur. J. Lipid Sci. Technol, 112, 1049-1056.
El-Naggar, N. E. A. A., El-Hersh, M. S., El-Fadaly, H. A. and Saber, W. I. (2011). Bioconversion of some agro-industrial by-products into single cell oil using Candida albicans NRRL Y-12983 and Lipomyces starkeyi NRRL Y-11557. Research Journal of Microbiology, 6(11), 784.
Gayathri, S., Prabhu, S., Mitra, A., Kumar, V., Sheela, J. S., Rajani, G. and Sivvaswamy, S. N. (2010). Soil microorganisms produce omega-3 fatty acids. Indian Journal of Science and Technology, 3(5), 499-503.
Gaykawad, S. S., Ramanand, S. S., Blomqvist, J., Zimmermann, B., Shapaval, V., Kohler, A. and Boccadoro, C. (2021). Submerged Fermentation of Animal Fat By-Products by Oleaginous Filamentous Fungi for the Production of Unsaturated Single Cell Oil. Fermentation, 7, 300.
Kusumawati, A. H., Wulan, I. R., & Ridwanuloh, D. (2020). Formulation and physical evaluation sheet mask from red rice (Oryza Nivara) and virgin coconut oil (Cocos Nucifera L). International Journal of Health & Medical Sciences, 3(1), 60-64. https://doi.org/10.31295/ijhms.v3n1.148
Maotsela, T., Danha, G. and Muzenda, E. (2019). Utilization of Waste Cooking Oil and Tallow for Production of Toilet “Bath” Soap. Procedia Manufacturing, 35, 541-545.
Murugan, T., Saravanan, D. and Balagurunathan, R. (2012). Production and optimization of single cell oil by oleaginous bacteria isolated from oil contaminated environment. Int J Current Resear. Rev., 4, 175-184.
Raslan, A. (2010). Biodiesel production from different methods. University of Birmingham.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Tamilalagan, A., Singaram, J. and Rajamohan, S. (2019). Generation of biodiesel from industrial wastewater using oleaginous yeast: performance and emission characteristics of microbial biodiesel and its blends on a compression injection diesel engine. Environmental Science and Pollution Research, 26, 11371-11386.
Torii, T., Kanemitsu, K., Wada, T., Itoh, S., Kinugawa, K. and Hagiwara, A. (2010). Measurement of short-chain fatty acids in human faeces using high-performance liquid chromatography: specimen stability. Annals of clinical biochemistry, 47, 447-452.
Yazdani, D., Zainal, A. M., Tan, Y. H. and Kamaruzaman, S. (2010). Evaluation of the detection techniques of toxigenic Aspergillus isolates. African Journal of Biotechnology, 9(45), 7654-7659.
Zhao, B., Wang, H., Xu, S., Qian, L., Li, H., Gao, J. and Xu, C. C. (2022). Influence of extraction solvents on the recovery yields and properties of bio-oils from woody biomass liquefaction in sub-critical water, ethanol or water–ethanol mixed solvent. Fuel, 307, 121930.
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