The antioxidant of Anethum graveolens (Dill)  on some biochemical parameter in blood serum of male rats

Dhuha Qasim; Linda H Al-Ghazali; Roaa Assim Hameed

doi:10.53730/ijhs.v6nS5.11672

Authors

Dhuha Qasim Ministry of Education, Directorate general of education in Karbala, Karbala in Iraq University of Warith Al-Anbiyaa, Karbala, Iraq
Linda H Al-Ghazali
linda.ahmeed@uokerbala.edu.iq
Department of clinical Laboratories, college of Applied Medical Sciences, university of kerbala, Iraq
Roaa Assim Hameed Ministry of Education, Directorate general of education in Babble, Babble in Iraq

Keywords:

Anethum graveolens (Dill), flavonoid, paracetamol

Abstract

Liver toxic is an acute effect of paracetamol (500 mg/kg) Consequently, we investigated how paracetamol toxicity affected liver cell enzymes in this study aspartate aminotransferase (GOT) and alanin aminotransferase catalyzes (GPT), Glutation (GSH( and total protein ,glucose , liver and body weight of male rats that drenched Anethum graveolens (Dill) . were used in this research 40 adult male rats, weight 172-311 gm, age 8-12 weeks, isolated in a environment at temperature 25ºC in an animal house in university of karbala ,They were given food, male rats were divided into four groups (G). (G1) the control group has gaves the food and normal saline 0,9% only, (G2) injection of paracetamol 500 mg/kg body weight (B.W). (G3) injection 100 mg/kg B.W A. graveolen extract + 500 mg/kg B.W of paracetamol. (G4) given 150mg/kg B.W of flavonoid compound extracted + 500 mg/kg B.W of paracetamol. The present study found ability of Dill and flavonoid extract to antioxidant and protective liver enzyme against the paracetamol overdose by significant decrease GPT, GOT, glucose.

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References

African pharmacopoeia. (1985). Lagos, Organization of African Unity, Scientific Technical and Research Commission Vol.1..

Al-Bazaz H.KH, Alnaqqash, Z.AB, Hasan.H.A. (2020).GC-Mass and Phytochemical Investigation of Iraqi Anethum graveolens L. Seeds. Mesopo. Environ. j, Vol. 5, No.4, pp.(44-50) , 2020.

AL-Hamzy,M.A.(2002)Effect of some medicinal plants extract on aggressive behavior of laboratory animals. JKAU. Sci.,14:13-23.

AL-Ibrahemi .N, AL-Laith.Z.N,AL-Yassiry.A and AL-Masaoodi N.H. (.2022).Chemical Analysis Of Phytochemical For the Anethum graveolens L. Fresh And Commercial Dry By Gas Chromatography Mass- Spectrometer.IOP.Conference series: Earth and Environmental Science.

AL-Ibrahemi .N; Hasan.R.M. (2019). Identification of Artemisinin compound in Artemisia herba alba belong to the Asteracea by HPLC and GC/MS. Al-Kufa University Journal for Biology. VOL.11 / NO.2. 2073-8854

AL-Ibrahemi .N; Hasan.R.M; Alslman.K.( 2020). Efect of Zinc oxid nanoparticles on the oxidant stress ( Malonaldehde MDA, lipid peroxidation level LPO) and antioxidant GSH glutation ) Medico-Legal Update 20(1), 882-888.

Almjalawi BSA, Alhamed TA, Almutteari AA. Antibacterial activity of biosurfactant extracted from Streptococcus thermophilus and its effect on some biochemical parameters in male rats. Caspian J Environ Sci. 2022;20(1):131-6.

AL-Saeedi AH, Hossain MA . (2015) . Total phenols , Total flavonoid contents and free radical scavenging activity of seeds crude extracts of pigeon pea traditionally used in oman for treatment of several chronic diseases . Asian pac J Trop Dis ; 5:316-321.

Anand B and Shrihari M. (2011). Evaluation of antioxidant properties of flower heads of Sphaeranthus indicus Linn. Indian Journal of Novel Drug delivery ; 3(2):118-124 .

Bergmeyer, H. U, Bernt E. (1974) Lactate-dehydrogenase, UV-assay with pyruvate and NADH. In: Bergmeyer.H. U. (ed.) Methods of enzymatic analysis, Vol 2. Academic Press, New York.; 574-579.

Burtis, C.A., and Ashwood, E.R. (1999)Tietz Textbook of Clinical Chemistry," 3rd ed., W.B. Saunders Company, Tokyo.1034-1054pp.

Burtis, C.A.; Ashwood, E.R. and Bruns, D.E.( 2012). Tietz textbook of clinical chemistry and Molecular Diagnostics. By saunders, an imprint of Elsevier Inc. USA.

Deaton, CM. and Marlin, D. (2003) Exercise associated oxidative stress. Clinical Techniques in Equine Practic. Vol. 189:278–291pp.

Delaquis PJ, Stanich K, Girard B, Mazza G. (2002). Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 74:101–109.

Ellman, G.L. (1959) Tissue sulfhydryl groups .Archives of Biochemistry and Biophysics.Vol. 82( 1) :70-77pp.

Enkvetchakul , B.; Bottje, W.; Anthony, N. and Moore, R. (1993) Compromised antioxidant status associated with ascites in broilers. Poultry Science. Vol.72: 2272-2280pp.

Geisman, T. A. (1962). Chemistry of flavonoids comopunds. Macmillan Co., NY.

Ghosh, A and Sil PC. (2009). Protection of acetaminophen induced mitochondrial dysfunctions and hepatic necrosis via Akt-NF-B pathway: Role of a novel plant protein. Chem Biol Interact.; 177: 96–106.

Harborne, J. B. (1984).Phytochemical Methods.; A Guide to ModernTechniques of Plant Analysis, 2nd ed. Chapman and Hall, London.

Jollow DJ, Thorgeirsson, Potter WZ, Hashimoto M, Mitchell JR. (1974). Acetaminophen induced hepatic necrosis VI. Metabolic disposition of toxic and non-toxic doses of acetaminophen. Pharmacology; 12:251-271

Kadhim, Sh.H. , Mosa, A.U , Ouda,M.H. (2013). Hepatoprotective effect of green tea (Camellia sinensis) on female rats drenched with paracetamol. Kerbala Journal of Pharmaceutical Sciences Number 6 .

Kapoor, B.B.; Mishra,R.; Acharya, S.; lakhera, S. and Purohit, V. (2013) Antimicrobial screening of some herbal plants of Rajasthan desert. Unique Journal of Engineering and Advanced Sciences. Vol.1(1):38-40pp.

Kaur G J, Arora D S. (2010). Bioactive potential of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi belonging to the family Umbelliferae - Current status. Journal of Medicinal Plants Research; 4(2): 87-94.

Kumar, V. and Yadav, S.K. (2009) Plantmediated synthesis of silver and gold nanoparticles and their applicationsJournal of Chemical. Technology and Biotechnology. Vol.84:151-7pp.

Kushwah,D.S.;Salman,M.T.; Singh,P.; Verma,V.K. and Ahmad,A.(2013). Protective effects of ethanolic extract of Nigella sativa seed in Paracetamol induced acut hepatotoxicity in vivo. Pakistan J. Biology Sciences; 10:3923.

Lewis,S.M.;Bain,B.J.and Bates,I.(2006).Dacie and Lewis practical heamatology ,10th edn., Elsevier, Germany: 709.

Li, Y.;Dankher, O. P.; Carreira, L.; Smith, A. P. and Meagher, R. B. (2006) The shoot-specific expression of γ-glutamylcysteine synthetase directs the long-distance transport of thiol-peptides to roots conferring tolerance to mercury and arsenic. Plant Physiology. 141:288–298pp.

Manal M. Ramadana, Nadia N. Abd-Algaderd, Hatil H. El-kamalid, Kadry Z. Ghanemb,e, Abdel Razik H. Farragc. (2013). Volatile compounds and antioxidant activity of the aromatic herb Anethum graveolens. Journal of The Arab Society for Medical Research • January 8:79–88.

Mates, J.M.; Perez-Gomes, C.; Nunez, D. and Castro, I. (1999) Antioxidant enzymes in human diseases. Clinical Biochemistry. Vol. 32(8): 595-603pp.

Mohammed, Zainab Y and Alsamarrae ,Khulood W. (2016) . Qualitative and Quantitative analysis of Lycium barbarum L. Flavonoid from leaves. Iraqi J Pharma Sci, vol, 25(2) .

Padmiswari, A. A. I. M., Wulansari, N. T., Antari, N. W. S., Damayanti, I. A. M., Indrayoni, P., & Indrawan, G. S. (2021). The effectiveness of soaking duration on blood cockles (Anadara granosa) with activated charcoal towards reducing metals lead (Pb). International Journal of Health & Medical Sciences, 4(3), 304-308. https://doi.org/10.21744/ijhms.v4n3.1756

Pandey, A.; Kaushik, A. and Tiwari, S. K. (2011). Evaluation of antimicrobial activity and phytochemical analysis of Citrus limon. J. of Pharmaceutical and Biomedical Scie. J.P.B.M.S. 13 (13): 1-7.

Pandey, N.; Chaurasia , J.; Tiwari, O. and Tripathi, Y.B. ( 2007) Antioxidant properties of different fractions of tubers from pueraria tuberosa Linna. Food chemistry. Vol.105:219-222pp.

Patel, J. A. and Shah, U. S. (2009). Bol. Latinoam Caribe Plant Med Aromat; 8: 121- 128.

Pingping Wu, Guangzhi Ma , Nianghui Li, Qian, D , Yanyan,Y, Ruqiang ,H. (2015) .Investigation of in vitro and in vivo antioxidant activities of flavonoid rich from the berries of rhodomyrtus tomentosa . Food Chem; 173 : 194-202.

Plaa GL, Hewitt WR,(Hayes AW, ed.), .(1982) New York: Raven Press; p. 407

Rakesh, S.U., Patil, P.R. and Mane, S.R. (2010) Use of natural antioxidants to scavenge free radicals: A major cause of diseases. International Journal of PharmTech Research. Vol.2:1074-1081pp.

Rice-Evan, C.A., Miller, N.J and Paganga, G. (1996) Structure antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine . Vol. 20:933-956pp.

Sai K, Takagi A, Umemura T, Hasegawa R, Kurokawa Y. (1992) . Changes of 8-hydroxydeoxyguanosine levels in rat organ DNA during the aging process. J Environ Pathol Toxicol Oncol; 11:139–143.

Savides Mc, Oehme FW. (1983). Acetaminophen and its toxicity. J Appl Toxicol; 3 : 95-111 .

Schmidt LE, Dalhoff K. (2002). Serum phosphate is an early predictor of outcome in severe acetaminophen-induced hepatotoxicity. Hepatology. Sep;36(3):659-65.

Stons , S.J; Bagchi , D.; Hassoun , E. and Bagchi M. (2001) oxidative mechanisim in the toxicity of chromium and cadmium ions.J. Environ. Pathol.Toxicol. oncol., 19: 201-213.

Su, K.; Yu, C. Y. ; Chn ,Y.W. , Huang,T. Y., ; Chen,C.T. ; Wu,H. F. and Chen S.Y.L. (2014) Rutin, a Flavonoid and principal Component of Saussurea involucrata , Attenuates physical fatigue in a Forced swimming Mouse Model . International Journal MedecineScience . Vol. 11(5): 528-537pp.

Sureshkumar SV, Mishra SH. (2007). Hepatoprotective activity of extracts from Pergularia daemia Forsk against carbon tetrachloride induced toxicity in rats. Pharmaocogn Mag; 3:187–191.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938

Wang, H.( 2010) . Effect of Astragalus Polysaccharides On Performance, Antioxidant Enzyme Activity, Immuntiy Enginery And Intestined Microflora Of Lying Hens. Master Thesis. Bao Ding: Agricultural University of Hebel .

Wong LT, Whitehouse LW, Solemonraj G, Paul CJ. (1981). Pathways of acetaminophen conjugate in the mouse. Toxicity Lett ; 9 : 145-151 .

Yakubu N, Oboh G, Olalekan AA. (2013) Antioxidant and hepatoprotective properties of tofu (curdle soymilk) against acetaminophen-induced liver damage in rats. Biotechnol Res Int; 1:1–7.