Genotyping of FOXO3a gene polymorphism rs13217795 (C > T) in asthmatic Iraqi patients

Thana Mohammed Juda; Seenaa Badr Mohammed; Ameera Jasim Al-Aaraji; Dhafer A. F. Al-Koofee

doi:10.53730/ijhs.v6nS6.11675

Authors

Thana Mohammed Juda University of Babylon, faculty of Medicine
Seenaa Badr Mohammed
bdr.seenae@uobabylon.edu.iq
University of Babylon, faculty of Medicine
Ameera Jasim Al-Aaraji University of Babylon, faculty of nursing
Dhafer A. F. Al-Koofee University of Kufa, faculty of Pharmacy

Keywords:

Asthma, FOXO3a, SNP, allele frequency, polymorphism, Iraq

Abstract

Background: Asthma is a chronic inflammatory process that affects the airways of the lung. It has a greater impact on public health. It is a polygenic disease, many factors play a role implicated in triggering its that starts like genetic, environmental, and geographic factors. Genetic studies have focused on the forkhead box O3 (FOXO3a) gene, mainly polymorphism at the specific sites which is thought may contribute to exacerbating the attack and complications of asthma. Methods: The study was designed as a case-control and involved 138 of asthmatic patients and 120 seemingly healthy people used as a control group. The diagnosis was carried out by consultants at a special respiratory center in Babylon governorate. Genotyping of all subjects was done for FOXO3a (rs13217795; C/T) analysis by PCR-RFLP technique. Multinominal regression was used to calculate Odds ratios (ORs) and 95% confidence intervals (CIs) by IBM. SPSS software version 20.0. Results: Analysis of the results that obtained form this work a considerable modification in forms of rs13217795; C/T polymorphism between asthmatic and healthy individuals. elevation in the risk of asmathic patient was seen in T allele of FOXO3a gene (odds ratio of 2.0313, 95% CI: 1.5493 - 2.6631, P<0.0001).

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