Molecular study of quorum sensing and biofilm formation genes in Pseudomonas aeruginosa isolated from UTIs patients

Ibtisam Habeeb Al-Azawi; Hiyam Abdulla Al-Shabbani

doi:10.53730/ijhs.v6nS4.11816

Authors

Ibtisam Habeeb Al-Azawi Department of Medical Microbiology, Collage of Medicine, University of Al-Qadisyiah, /Iraq
Hiyam Abdulla Al-Shabbani Department of Medical Microbiology, Collage of Medicine, University of Al-Qadisyiah, /Iraq

Keywords:

UTIs, P. aeruginosa, quorum sensing, biofilm

Abstract

Background: Urinary tract infection (UTI) is a common health problem in both community and nosocomial settings, affecting both men and women equally. Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Biofilm is an architecture built mostly by autogenic extracellular polymeric substances which function as a scaffold to encase the bacteria together on surfaces, and to protect them from environmental stresses, impedes phagocytosis and thereby conferring the capacity for colonization and long-term persistence. So, the aim of this study to screen of some important quorum sensing and biofilm genes among pseudomonas aeruginosa isolated from UTIs patients. Methods: These study was conducted in Al-Qadisiyah province, Iraq at five major hospitals (AL-Diwanyia Teaching Hospital, Feminine and children teaching hospital, Afak General Hospital, AL-Hamzah General Hospital and AL-Shamiya General Hospital) during the period from (November, 2020 to June, 2021). A total of 800 urine samples were collected from male and females referring to five major hospitals. The age of the patients ranged from (1 to 80) years-old. Results: Sixty isolates were showed positive and identified as P. aeruginosa by using selective media, biochemical test system and VITEK-2 compact system.

Downloads

Download data is not yet available.

References

Abdelraheem, W. M., Abdelkader, A. E., Mohamed, E. S., & Mohammed, M. S. (2020). Detection of biofilm formation and assessment of biofilm genes expression in different Pseudomonas aeruginosa clinical isolates. Meta Gene, 23, 100646.

Abdullah, W. M., & Bunyan, I. A. (2022). Study the Molecular Markers of Quorum Sensing in Pseudomonas aeruginosa Isolated from Different Clinical Infections. Journal of Complementary Medicine Research, ISSN: 2146-8397 Vol. 13, No. 1, 2022 (pp. 102-106).

Abdul-Wahid, Ali, A. (2020). Antibiotic Sensitivity and Molecular Profile of Carbapenemase-Producing Pseudomonas aeruginosa from hospital-acquired infections in Thi-Qar province. PhD. Thesis, College of Medicine, University of Al-Qadissiyah.

Abosaooda, M., Wajdy, J. M., Hussein, E. A., Jalil, A. T., Kadhim, M. M., Abdullah, M. M., ... & Almashhadani, H. A. (2021). Role of vitamin C in the protection of the gum and implants in the human body: theoretical and experimental studies. International Journal of Corrosion and Scale Inhibition, 10(3), 1213-1229. https://dx.doi.org/10.17675/2305-6894-2021-10-3-22

Al-Janahi, Haider C. L. (2020). Occurrence and Molecular Characterization of Metallo-β-Lactamase (MBLs)-Producing Pseudomonas aeruginosa in Najaf Hospitals. MSc. Thesis, Faculty of Medicine, University of Kufa.

Al-kazrage, Hussein A. D. (2021). Inhibition of Virulence Factors in Pseudomonas aeruginosa Isolated from Clinical Samples Using Galardin Loaded AgPEG Nanocomposite. PhD. Thesis, College of Biotechnology, University of Al-Nahrain.

Al-Sheikhly, M. A., Musleh, L. N., and Al-Mathkhury, H. J. F. (2019). Assessment of pelA-carried Pseudomonas aeruginosa isolates in respect to biofilm formation. Iraqi Journal of Science, 60(6), 1180–1187.

Belal, E. J. K. (2010). Investigation of some B-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in Najaf City. M.Sc. Thesis. Edication of Girls/ University of Kufa. Iraq.

Choi, Y.; Park, H.-Y.; Park, S.J.; Park, S.-J.; Kim, S.-K.; Ha, C.; Im, S.-J.; Lee, J.-H. (2011). Growth phase-differential quorum sensing regulation of anthranilate metabolism in Pseudomonas aeruginosa. Mol. Cells, 32, 57–65.

Chowdhury, N., & Bagchi, A. (2016). Molecular insight into the activity of LasR protein from Pseudomonas aeruginosa in the regulation of virulence gene expression by this organism. Gene, 580(1), 80-87.

Clinical and Laboratory Standards Institute (CLSI). (2020). Performance standards for antimicrobial susceptibility testing. CLSI supplement M100, 30th. Ed. Clinical and Laboratory Standards Institute, Wayne, PA.

Colvin, K.M., Irie, Y., Tart, C.S., Urbano, R., Whitney, J.C., Ryder, C., Howell, P.L., Wozniak, D.J. and Parsek, M.R. (2012). The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix. Environ. Microbiol, 14: 1913–1928.

Crespo, A.; Blanco-Cabra, N.; Torrents, E. (2018). Aerobic Vitamin B12 Biosynthesis Is Essential for Pseudomonas aeruginosa Class II Ribonucleotide Reductase Activity During Planktonic and Biofilm Growth. Front. Microbiol., 9, 986.

Daragon, B., Fournier, D., Plésiat, P., & Jeannot, K. (2021). Performance of disc diffusion, MIC gradient tests and Vitek 2 for ceftolozane/tazobactam and ceftazidime/avibactam susceptibility testing of Pseudomonas aeruginosa. Journal of Antimicrobial Chemotherapy, 76(10), 2586-2592.

Dunne, M. W., Puttagunta, S., Aronin, S. I., Brossette, S., Murray, J., & Gupta, V. (2022). Impact of Empirical Antibiotic Therapy on Outcomes of Outpatient Urinary Tract Infection Due to Nonsusceptible Enterobacterales. Microbiology spectrum, 10(1), e02359-02321.

Foxman, B. (2020). Urinary tract infection syndromes: occurrence, recurrence, bacteriology, risk factors, and disease burden. Infect. Dis. Clin. North America., 28 (1):1-13.

Galdino, A. C. M., Branquinha, M. H., Santos, A. L., & Viganor, L. (2017). Pseudomonas aeruginosa and its arsenal of proteases: weapons to battle the host. Pathophysiological Aspects of proteases, 381-397.

Ghafoor, A.; Hay, I.D.; Rehm, B.H. (2011). Role of exopolysaccharides in Pseudomonas aeruginosa biofilm formation and architecture. Appl. Environ. Microbiol., 77, 5238–5246.

Gomila, A.; Carratalà, J.; Badia, J.M.; Camprubí, D.; Piriz, M.; Shaw, E.; Diaz-Brito, V.; Espejo, E.; Nicolás, C.; Brugués, M.; et al. (2018). Preoperative oral antibiotic prophylaxis reduces Pseudomonas aeruginosa surgical site infections after elective colorectal surgery: A multicenter prospective cohort study. BMC Infect. Dis, 18, 507.

Jalil, A. T., Shanshool , M. T. ., Dilfy, S. H. ., Saleh, M. M., & Suleiman, A. A. . (2022). HEMATOLOGICAL AND SEROLOGICAL PARAMETERS FOR DETECTION OF COVID-19. Journal of Microbiology, Biotechnology and Food Sciences, e4229. https://doi.org/10.15414/jmbfs.4229

Jeske, A., Arce-Rodriguez, A., Thöming, J. G., Tomasch, J., & Häussler, S. (2022). Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates. NPJ biofilms and microbiomes, 8(1), 1-14.

Jumintono, J., Alkubaisy, S., Yánez Silva, D., Singh, K., Turki Jalil, A., Mutia Syarifah, S., ... & Derkho, M. (2021). Effect of Cystamine on Sperm and Antioxidant Parameters of Ram Semen Stored at 4° C for 50 Hours. Archives of Razi Institute, 76(4), 923-931. https://dx.doi.org/10.22092/ari.2021.355901.1735

Kim, E. M., Woo, H. M., Tian, T., Yilmaz, S., Javidpour, P., Keasling, J. D., & Lee, T. S. (2017). Autonomous control of metabolic state by a quorum sensing (QS)-mediated regulator for bisabolene production in engineered E. coli. Metabolic engineering, 44, 325-336.

Lahij, H. F., Alkhater, A. H., Hassan, M. H., & Yassir, L. A. (2021). The Effect of Quorum Sensing genes (lasI, rhlI) in Some Virulence Factors of Pseudomonas aeruginosa Isolated From Different Clinical Sources. Medico Legal Update, 21(1), 303-308.

Lee, J.; Zhang, L. (2015). The hierarchy quorum sensing network in Pseudomonas aeruginosa. Protein Cell, 6, 26–41.

Li, Y.; Petrova, O.E.; Su, S.; Lau, G.W.; Panmanee, W.; Na, R.; Hassett, D.J.; Davies, D.G.; Sauer, K. (2018). BdlA, DipA and Induced Dispersion Contribute to Acute Virulence and Chronic Persistence of Pseudomonas aeruginosa. PLoS Pathog., 10, e1004168.

MacFaddin, J.F. (2004). Biochemical tests for identification of medical bacteria (7rd ed.), Lippincott Williams and Wilkins, USA.

Maita, P., & Boonbumrung, K. (2017). Association between biofilm formation of Pseudomonas aeruginosa clinical isolates versus antibiotic resistance and genes involved with biofilm. Journal of Chemical and Pharmaceutical Research, 6, 1022-1028.

Malešević, M., Di Lorenzo, F., Filipić, B., Stanisavljević, N., Novović, K., Senerovic, L. & Jovčić, B. (2019). Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia

tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. Scientific reports, 9(1), 1-13.

Marofi, F., Abdul‐Rasheed, O. F., Rahman, H. S., Budi, H. S., Jalil, A. T., Yumashev, A. V., ... & Jarahian, M. (2021). CAR‐NK cell in cancer immunotherapy; A promising frontier. Cancer Science, 112(9), 3427. https://doi.org/10.1111/cas.14993

Marofi, F., Rahman, H. S., Al-Obaidi, Z. M. J., Jalil, A. T., Abdelbasset, W. K., Suksatan, W., ... & Jarahian, M. (2021). Novel CAR T therapy is a ray of hope in the treatment of seriously ill AML patients. Stem Cell Research & Therapy, 12(1), 1-23. https://doi.org/10.1186/s13287-021-02420-8

McDonald, J.H. (2014). Handbook of Biological Statistics. 3rd ed. USA: Sparky House Publishing.

Moghadasi, S., Elveny, M., Rahman, H. S., Suksatan, W., Jalil, A. T., Abdelbasset, W. K., ... & Jarahian, M. (2021). A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine. Journal of Translational Medicine, 19(1), 1-21. https://doi.org/10.1186/s12967-021-02980-6

Moradali, M.F.; Ghods, S.; Rehm, B.H. (2017). Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence. Front. Cell Infect. Microbiol, 7, 39.

Muderris, T.; Durmaz, R. and Ozdem, B. (2018). Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates. J Infect Dev. Ctries. 12(1):1–8.

Mukherjee, S.; Bassler, B.L. (2019). Bacterial quorum sensing in complex and dynamically changing environments. Nat. Rev. Genet, 17, 371–382.

NGAFWAN, N., RASYID, H., ABOOD, E. S., ABDELBASSET, W. K., Al-SHAWI, S. G., BOKOV, D., & JALIL, A. T. (2021). Study on novel fluorescent carbon nanomaterials in food analysis. Food Science and Technology. https://doi.org/10.1590/fst.37821

Oluyombo, O.; Penfol. M d, C.N.; Diggle, S.P. (2019). Competition in Biofilms between Cystic Fibrosis Isolates of Pseudomonas aeruginosa Is Shaped by R-Pyocins. mBio, 10, e01828-18.

Pang, Z.; Raudonis, R.; Glick, B.R.; Lin, T.J.; Cheng, Z. (2019). Antibiotic resistance in Pseudomonas aeruginosa: Mechanisms and alternative therapeutic strategies. Biotechnol. Adv., 37, 177–192.

Rampioni, G.; Falcone, M.; Heeb, S.; Frangipani, E.; Fletcher, M.P.; Dubern, J.-F.; Visca, P.; Leoni, L.; Cámara, M.; Williams, P. (2016). Unravelling the Genome-Wide Contributions of Specific 2-Alkyl-4-Quinolones and PqsE to Quorum Sensing in Pseudomonas aeruginosa. PLoS Pathog., 12, e1006029.

Ratnawati, I. G. A. A., Suandayani, N. K. T., & Sutapa, G. N. (2019). The linearity of x-ray devices radiation output and its relationship with patient thickness. International Journal of Physical Sciences and Engineering, 3(3), 1–6. https://doi.org/10.29332/ijpse.v3n3.351

Romling, U.; Balsalobre, C. (2012). Biofilm infections, their resilience to therapy and innovative treatment strategies. J. Intern. Med, 272, 541–561.

Saleh, M. M., Jalil, A. T., Abdulkereem, R. A., & Suleiman, A. A .(2020). Evaluation of Immunoglobulins, CD4/CD8 T Lymphocyte Ratio and Interleukin-6 in COVID-19 Patients. TURKISH JOURNAL of IMMUNOLOGY, 8(3), 129-134. https://doi.org/10.25002/tji.2020.1347

Sarjito, Elveny, M., Jalil, A., Davarpanah, A., Alfakeer, M., Awadh Bahajjaj, A. & Ouladsmane, M. (2021). CFD-based simulation to reduce greenhouse gas emissions from industrial plants. International Journal of Chemical Reactor Engineering, 20210063. https://doi.org/10.1515/ijcre-2021-0063

Senturk, S., Ulusoy, S., Bosgelmez-Tinaz, G. and Yagci, A. (2019). Quorum sensing and virulence of Pseudomonas aeruginosa during urinary tract infections. J Infect Dev Ctries 6, 501– 507.

Sharma, I., & Choudhury, D. (2017). Detection of PelA gene in P. aeruginosa from clinical samples using polymerase chain reaction with reference to biofilm production in NE India. Ind J Res, 4, 119-121.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Susanti, N. L. P. D., Yuliana, E. D., & Suwardani, N. P. (2021). Management sewakadharma nurses in wangaya regional public hospitals. International Research Journal of Management, IT and Social Sciences, 8(3), 306-314. https://doi.org/10.21744/irjmis.v8n3.1717

Turki Jalil, A., Hussain Dilfy, S., Oudah Meza, S., Aravindhan, S., M Kadhim, M., & M Aljeboree, A. (2021). CuO/ZrO2 nanocomposites: facile synthesis, characterization and photocatalytic degradation of tetracycline antibiotic. Journal of Nanostructures. https://doi.org/10.22052/JNS.2021.02.014

Vakili-Samiani, S., Jalil, A. T., Abdelbasset, W. K., Yumashev, A. V., Karpisheh, V., Jalali, P., ... & Jadidi-Niaragh, F. (2021). Targeting Wee1 kinase as a therapeutic approach in Hematological Malignancies. DNA repair, 103203. https://doi.org/10.1016/j.dnarep.2021.103203

Widjaja, G., Jalil, A. T., Rahman, H. S., Abdelbasset, W. K., Bokov, D. O., Suksatan, W., ... & Ahmadi, M. (2021). Humoral Immune mechanisms involved in protective and pathological immunity during COVID-19. Human Immunology. https://doi.org/10.1016/j.humimm.2021.06.011

Yan, S., & Wu, G. (2019). Can biofilm be reversed through quorum sensing in Pseudomonas aeruginosa?. Frontiers in microbiology, 10,1582.

Zhou, J. W., Luo, H. Z., Jiang, H., Jian, T. K., Chen, Z. Q., & Jia, A. Q. (2018). Hordenine: a novel quorum sensing inhibitor and antibiofilm agent against Pseudomonas aeruginosa. Journal of agricultural and food chemistry, 66(7), 1620-1628.