Extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum
Keywords:
extraction, purification, glucose oxidase enzyme, Penicillium chrysogenumAbstract
The study included the extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum, pure of the enzyme by precipitation with ammonium sulfate at a saturation rate of 70% The specific activity in this step reached (10.802) units/mg and fold 1.276, and this was followed by the dialysis step, and the specific activity was 13,593 units/mg and fold 48.184. Finally, ion exchange chromatography using the DEAE-Cellulose column, the specific activity reached 19.355 units/mg, and fold 36.59, after which the enzyme was migrated by the electrophoresis device and one protein bundle appeared.
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