Detection of acquired resistance genes for vancomycin in local Iraqi isolates of Klebsiella pneumoniae

Shahad Hisham Mahmood; Ahmed Mohamed; Ilham Abdul Hadi Khalaf

doi:10.53730/ijhs.v6nS6.12204

Authors

Shahad Hisham Mahmood
shahad_eye@yahoo.com
Department of Biotechnology, College of Applied Science, University of Fallujah, Fallujah-Iraq
Ahmed Mohamed Department of Biology, College of Sciences, University of Anbar, Anbar-Iraq
Ilham Abdul Hadi Khalaf Al-Razi Center for Research and Diagnostic Kits, Ministry of Industry and Minerals, Baghdad-Iraq

Keywords:

Klebsiella pneumonia, VanA, VanB, PCR, molecular study

Abstract

The species were collected from 3 main hospitals in different regions in Baghdad, and presented by one hundred and seventy-four for various genders and ages. The collected sources were 60 samples of urine, 33 samples of wounds, 36 samples of burns, 45 sample of fecal material. A total 60 isolates were isolated and diagnosed according to microscopic, cultural and biochemical tests as well as Api – 20 E, VITEK2 Compact systems were performed as well as specific 16SrRNA gene was applied for molecular identification of Klebsiella pneumoniae isolates deposited in the National Center for Biotechnology Information (NCBI) (From LC314482.1 - LC314486.1). As a result of these tests a total of 13 Klebsiella pneumoniae isolates were isolated and identified. For the precise detection of studied isolates in molecular techniques, chromosomal DNA was extracted. It was clear that the percentage of VanA gene appearance was 40% and 6.66% VanB gene for the selected isolates. Vancomycin-resistant genes were investigated for these bacterial isolates using specific primers for the resistant genes (VanA & VanB). Results of gel electrophoresis of the PCR products for Klebsiella pneumoniae that they have the resistant genes (VanA & VanB) appeared on chromosomal DNA.

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