Alveolar gene expression of tight junction protein in nicotine rats treated with zinc and vitamin D
Keywords:
Nicotine, zinc, vitamin D, tight junction protein, ferritin and lungAbstract
The current study aimed to investigation the role of zinc and vitamin D in modulating alveolar response of nicotine stress as a model of mammals male Wistar rats. Thirty mature males werekept at 23 ± 2 C°, randomly assigned to five equal groups and treatment for 14 days, C = Control drenched vehicle without treatment, G1 = injected with i/p nicotine 1.5 mg/ kg b.w., G2 = administrated orally of zinc 60 mg/ kgb.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w., G4 = administrated orally both of zinc and vitamin D with same doses stressed and nicotine 1.5 mg/ kgb.w.i/p. Rats were anesthetized with ketamine at the end of the treatment period with xylazine and blood samples have been collected from the optical vein for estimation of serum ferritin and transferrin, then animals were sacrificed and lung was removed and weighted.All groups of rats had lung samples extracted rapidly, dipped in DEPC solution, and frozen under liquid nitrogen for determination of tight junction protein ( TJP) gene expression by RT-PCR analyses. Nicotine-stressed rats treated with zinc and vitamin D (G4) havesignificant highly increased expression of alveolar tight junction protein when compared with nicotine group (G1).
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