Evaluation of the ability of some local soil fungi to produce oxalic acid under different conditions
Keywords:
oxalic acid, A.niger, local soil fungiAbstract
The aim of this study was to isolate fungi from soil and evaluate their ability to produce oxalic acid, then determine the most productive fungal isolate and the optimal conditions for acid production. All fungal isolates were oxalate producers, and the most efficient fungal species was A.niger, followed by A.flavus, then Rizoctonia solani with production rates of 4.05% , 3.54% , and 3.42% respectively , the rest isolates gave variable productivity. The optimum conditions for oxalate production were as follows ; (4%) sucrose as the best carbon source, (0.25%) ammonium sulfate as a best nitrogen source, (0.1%) hydrated magnesium sulfate as a best salt , (3%) which is equal to 1×106 spores/ml was the best inoculum size, the optimal pH value was equal to 6.5 , and 25°C represent an optimal temperature degree , for 7 days incubation period.
Downloads
References
Afzal, H. ; Shazad, S. ,and Nisa, S.Q.U. (2013) . Morphological identification of Aspergillus species from the soil of Larkana District (Sindh, Pakistan ). Asian Journal of Agriculture and Biology , 1(3): 105-117.
Al- Khalil , Abdullah bin Saleh bin Hassan. (2005). The scientific basis of fungi. King Saud University . Second Edition . Riyadh .
Al-Jeriawi, Huda Abbas Mohammed Mikheef and Al-Mamouri, Zidan Khalif Omran. (2015). Effect of ultraviolet rays on the vitality of spores of Aspergillus niger Van Tieghem. Journal of Babylon University for Pure and Applied Sciences, Volume (23), Issue 1: 154-161.
Al-Mehana, V.N.A.; Al-Shibly, M.K. & Al-Daemi, F.H.F.(2021). Optimal conditions for oxalic acid production by Aspergillus niger and A. flavus. Iranian Journal of Ichthyology 8(ICAEAS 2021): 138-144.
Al-Muzaffar, Haider Abdel Moneim. (2013). Diagnosis of Sclerotinia sclerotiorum strain using PCR and estimation of oxalic acid produced under different environmental and chemical conditions. College of Education for Pure Sciences / University of Karbala. Al-Furat Journal of Agricultural Sciences - 5 (3) 179-192.
Bateman, D. F. & Beer, S. V. (1965). Simultaneous production and Synergistic action of oxalic acid and poly galacturonase during pathogenesis by Sclerotium rolfsii, Phytopathology, 55: 204 –211.
Bryan, J. ; Culbertson, B. J. ; Krone, J. ; Gatebe, E. ; Furumo, N. C. & Daniel, S. L. (2007). Impact of carbon sources on growth and oxalate synthesis by the phytopathogenic fungus Sclerotinia sclerotiorum . World J Microbial Biotechnol.
Caliskan , M . ( 2000 ) . The metabolism of oxalic acid . Turk . J . zool . 24 : 103 – 106 .
Carmen, V. and Sciortion . J.(2017). Atlas of clinically important fungi . Hobekan, New Jersey : John Wiley &Sons, Inc.
Clausen , C . A . ( 2000 ) . CCA removal from treated Wood using adual remediationj process . Wast Management and Research , 18 : 485 – 488 .
Demain , A . & Dana , C . A . ( 2007 ) . the business of Biotechnology . Ind . Biotechnol . J , 3 ( 3 ) : 269 – 283 .
Ellis, D. H. (1994). Clinical Mycology : the human opportunistic . mycosis . , Gillingham printers pty. Ltd. Australia: pp 166 .
Gilberto de , O . M . ; Hiunes , M . M . and Rafael , V . V . ( 2020 ) . Oxalic acid is more efficient than sulfuric acid for rock phosphate solubilization Miner . Eng .
Gilberto de , O . M . ; Hiunes , M . M . and Rafael , V . V . ( 2020 ) . Oxalic acid is more efficient than sulfuric acid for rock phosphate solubilization Miner . Eng .
Hart, H. ( 1983 ). Organic chemistry . 6th Ed., Houghton Mifflin Company, USA .
Hodgkinson , A . ( 1977 ) . Oxalic acid in Biology and Medicine . Academic press , London .
Katherine , B . ; Joe , H . and Keith , B . ( 2017 ) . Production of oxalic acid from Aspergillus niger and whey permeate . water Air soil pollut ( 2018 ) 229 : ( 5 ) p : 4 – 10.
Kidd, S.; Halliday , C.L.; Alexious, H. and Ellis, D.H. (2016). Descriptions medical fungi .David Ellis.
Kown – chung , K . J . & Bennett , J . E . ( 1992 ) . Medical Mycology . Philadelphia , lea and Febiqer . p : 866 .
Lacey , A . L . ( 1997 ) . Manual of techniques insect pathology . Academic press , New York , pp : 410 – 411 .
Leangon, S.; Maddox, I.S. & Brooks, J.D.(2000). A proposed biochemical mechanism for citric acid accumulation by Aspergillus niger Yang No.2 growing in solid state fermentation. W. J. Microbiol. Biotechnol, 16: 271- 275.
Maxwell, D. P. & Lumsden, R. D. (1970). Oxalic acid production by Sclerotinia sclerotiorum in infected bean and in culture, Phytopathology, 60: 1395 – 1398.
Micales , J . A . ( 1995 ) . In Vitro oxalic acid production by the brown – rot fungus Postia placenta . Material und organism , 29 : 159 – 176 .
Munir , E . ; Yoon , J . J . ; Takamatsu , T . ; Hattori , T . and Shimada , M . ( 2001 ) . A physiological role for oxalic acid biosynthesis in the wood - rotting basidiomycetes Fomitopsis palustris . PNAS online , 98 ( 20 ) : 11162 – 11130.
Mwangi, E. S. K. ; Gatebe, E. G. & Ndung’u, M. W. (2012). Impact of nutritional (C:N ratio and source) on growth, oxalate accumulation, and culture pH by Sclerotinia sclerotiorum , Journal of Biology, Agriculture and Healthcare, 2(10): 2224 – 3208.
Papagianni , M . ( 2007 ) . Advances in citric acid permentation by Aspergillus niger Biochemical aspects , membrane transport and modeling . Biotechnol . Adv , 25 : 244 – 263 .
Podgorski , W . and Lesniak , W . ( 2003 ) . oxalic acid production by Aspergillus niger , Tatranske Mattiare, Slovak republic ( Abstract ) .
Ramli, R. M., Sinrang, A. W., & Aminuddin. (2021). Levels of alpha-1 acid glycoprotein (AGP) in stunting and non stunting tolls age 36-60 months . International Journal of Health & Medical Sciences, 4(1), 145-149. https://doi.org/10.31295/ijhms.v4n1.1666
Sahin , N . ( 2004 ) . Decomposition of oxalate by Microorganisms , 6th update , from site WWW. Geocities . com ( Internet ) .
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864
Wei, C.I. ; Huang, T.S. ; Chen, J.S. ; Marshall, M.R. and Chung, K.T.(1991). Production of kojic acid by Aspergillus candidus in three culture media. J. Food Prot. 54: 546±548.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
