Therapeutic efficiency of probiotic lactobacillus acidophilus, lactobacillus rhamnosus and antibiotic against isolates of pathogenic bacterial from skin wound infections
Keywords:
Lactobacillus acidophilus, Lactobacillus rhamnosus, probiotic, biofilm formation, antimicrobial agents, skin wound infectionsAbstract
Background: Probiotic refer to harmless live normal flora micro-organisms (M.O), which are mostly strains of Lactobacillus spp., Bifidobacterium spp., and others. These microorganisms (M.O) help the host's health when given in the right dosages. Aims: To identify bacterial species isolated from wound infections, and also to evaluate in-vitro the antimicrobial activity of selected probiotic species and antibiotic against some pathogenic bacteria. Methods: Records of wound swabs collected from 102 patients with high suspicion of wound infections. Bacterial colonies were Gram stained and microscopically examined. Biochemical tests were done to identify pathogen species. The microtitre plate method (MTP) was used to detect biofilm formation by bacteria. The Kirby-Bauer disk diffusion method was used for antibiotic testing. The well diffusion method and agar spot method was used for detection probiotic affectivity to a group of bacteria isolated (totally 38 isolates). Results: Prevalence of wound infections was 81.4% (CI: 60.869). A total of 10 species were isolated from 83 infected wounds. The most common bacterial species detected was Staphylococcus aureus (26.5%), followed by Pseudomonas aeruginosa 14.4%, Proteus mirabilis (12%), Escherichia coli 10.8%, and Enterobacter cloacae (8.4%). Polymicrobial infection was found in 25(30.1%) of the samples and was mainly constituted with two species.
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Abd El-Baky, R. M., Ibrahim, R. A., Mohamed, D. S., Ahmed, E. F., and Hashem, Z. S. (2020). Prevalence of virulence genes and their association with antimicrobial resistance among pathogenic E. coli isolated from Egyptian patients with different clinical infections. Infection and Drug Resistance, 13, 1221.
Abriouel, H., Benomar, N., Cobo, A., Caballero, N., Fuentes, M. Á. F., Pérez-Pulido, R., and Gálvez, A. (2012). Characterization of lactic acid bacteria from naturally-fermented Manzanilla Aloreña green table olives. Food microbiology, 32(2), 308-316.
Al-Azzawi, M. K., Makharmash, J. H., and Al-Malkey, N. K. (2020). The effect of Lactobacillus species on the Pseudomonas aeruginosa. Drug Invention Today, 14(2).
al-Husni, Halah Muhammad Husayn. (2011). Comparative study between methicillin-resistant coagulase positive and negative Staphylococci. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq.
Al-Malkey, M. K., Ismeeal, M. C., Al-Hur, F. J. A., Mohammed, S. W., and Nayyef, H. J. (2017). Antimicrobial effect of probiotic Lactobacillus spp. On Pseudomonas aeruginosa. Journal of Contemporary Medical Sciences, 3(10), 218-223.
Atiyeh, B. S., and Al-Amm, C. A. (2001). Immunology of burn injury-an overview. Annals of Burns and Fire Disasters, 14(2), 78-84.
Avila-Novoa, M. G., Iñíguez-Moreno, M., Solís-Velázquez, O. A., González-Gomez, J. P., Guerrero-Medina, P. J., and Gutiérrez-Lomelí, M. (2018). Biofilm formation by Staphylococcus aureus isolated from food contact surfaces in the dairy industry of Jalisco, Mexico. Journal of Food Quality, 2018.
Bahramian, A., Khoshnood, S., Shariati, A., Doustdar, F., Chirani, A. S., and Heidary, M. (2019). Molecular characterization of the pilS2 gene and its association with the frequency of Pseudomonas aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island. Infection and Drug Resistance, 12, 221.
Bowler, P. G., Duerden, B. I., and Armstrong, D. G. (2001). Wound microbiology and associated approaches to wound management. Clinical microbiology reviews, 14(2), 244-269.
Bowler, P. G., Duerden, B. I., and Armstrong, D. G. (2001). Wound microbiology and associated approaches to wound management. Clinical microbiology reviews, 14(2), 244-269.
Burnham, J. P., Kirby, J. P., and Kollef, M. H. (2016). Diagnosis and management of skin and soft tissue infections in the intensive care unit: a review. Intensive care medicine, 42(12), 1899-1911.
Cardona, A. F., and Wilson, S. E. (2015). Skin and soft-tissue infections: a critical review and the role of telavancin in their treatment. Clinical Infectious Diseases, 61(suppl_2), S69-S78.
Christensen, G. D., Simpson, W. A., Younger, J. J., Baddour, L. M., Barrett, F. F., Melton, D. M., and Beachey, E. H. (1985). Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of clinical microbiology, 22(6), 996-1006.
Fijan, S., Frauwallner, A., Langerholc, T., Krebs, B., ter Haar née Younes, J. A., Heschl, A., ... and Rogelj, I. (2019). Efficacy of using probiotics with antagonistic activity against pathogens of wound infections: an integrative review of literature. BioMed research international.
Flayyih, May and Abu-Risha, Rasmyia and Hasan, Jinan. (2012). The Prophylactic Role of Lipopolysaccharide of Pseudomonas aeruginosa Against Corneal. Infection Journal of Biotechnology Research Center. 6. 96-101.
Foster, T. J. (2002). Staphylococcus aureus. Molecular Medical Microbiology, 839-888.
Glik, J., Kawecki, M., Gaździk, T., and Nowak, M. (2012). The impact of the types of microorganisms isolated from blood and wounds on the results of treatment in burn patients with sepsis. Polski Przeglad Chirurgiczny, 84(1), 6-16.
Guo, B., Dong, R., Liang, Y., and Li, M. (2021). Haemostatic materials for wound healing applications. Nature Reviews Chemistry, 5(11), 773-791.
Herek, O., OZTURK, H., Ozyurt, M., Albay, A., and Cetinkursun, S. (2000). Effects of treatment with immunoglobulin on bacterial translocation in burn wound infection. Annals of Burns and Fire Disasters, 13(1), 13-17.
Holzapfel, W. H. N., and Wood, B. J. (2012). The genera of lactic acid bacteria (Vol. 2). Springer Science and Business Media.
James, C., and Natalie, S. (2014). Microbiology. A laboratory manual. Pearson Education.
Jebur, M. S. (2010). Therapeutic efficacy of Lactobacillus acidophilus against bacterial isolates from burn wounds. North American journal of medical sciences, 2(12), 586.
Jijon H, Backer J, Diaz H, et al. (2004). DNA from probiotic bacteria modulates murine and human epithelial and immune function. Gastroenterology; 126:1358- 1373.
Kazou, M., Grafakou, A., Tsakalidou, E., and Georgalaki, M. (2021). Zooming into the microbiota of home-made and industrial kefir produced in Greece using classical microbiological and amplicon-based metagenomics analyses. Frontiers in Microbiology, 12, 621069.
Komolafe, O. O., James, J., Kalongolera, L., and Makoka, M. (2003). Bacteriology of burns at the queen elizabeth central hospital, Blantyre, Malawi. Burns, 29(3), 235-238.
Kusumawati, A. H., Wulan, I. R., & Ridwanuloh, D. (2020). Formulation and physical evaluation sheet mask from red rice (Oryza Nivara) and virgin coconut oil (Cocos Nucifera L). International Journal of Health & Medical Sciences, 3(1), 60-64. https://doi.org/10.31295/ijhms.v3n1.148
Lari, A. R., Alaghehbandan, R., and Akhlaghi, L. (2005). Burn wound infections and antimicrobial resistance in Tehran, Iran: an increasing problem. Annals of burns and fire disasters, 18(2), 68.
Macedo, J. L. S. D., Rosa, S. C., and Castro, C. (2003). Sepsis in burned patients. Revista da Sociedade Brasileira de Medicina Tropical, 36, 647-652.
Marquès, C., Tasse, J., Pracros, A., Collin, V., Franceschi, C., Laurent, F., ... and Forestier, C. (2015). Effects of antibiotics on biofilm and unattached cells of a clinical Staphylococcus aureus isolate from bone and joint infection. Journal of medical microbiology, 64(9), 1021-1026.
Mc-Cracken BJ, Gaskins HR. (1999). Probiotics and the immune system. In: Tannok GW(ed.): probiotics: a critical review. Norfolk, Va: Horizon Scientific Press; 85-111.
Modaresifar, K., Azizian, S., Ganjian, M., Fratila-Apachitei, L. E., and Zadpoor, A. A. (2019). Bactericidal effects of Nano patterns: A systematic review. Acta Biomaterialia, 83, 29-36.
Murray, P. M., and Finegold, S. M. (1984). Anaerobes in burn-wound infections. Reviews of infectious diseases, 6(Supplement_1), S184-S186.
Nowroozi, J., Mirzaii, M., and Norouzi, M. (2004). Study of Lactobacillus as probiotic bacteria. Iranian Journal of Public Health, 33(2), 1-7.
Oleiwi, Sanaa and Suhail, Shaymaa and Radif, Hala and Doi,. (2021). Morphological and Molecular Study of Biofilm Formation by Enterobacter cloacae. Annals of Tropical Medicine and Public Health. 24. 176-186.
Paongphan, P., and Taweechotipatr, M. (2019). Characterization of probiotic lactic acid bacteria producing bile-salt hydrolase for development of fermented milk product (Doctoral dissertation, Srinakharinwirot University), 8-22.
Salas-Jara, M. J., Ilabaca, A., Vega, M., and García, A. (2016). Biofilm forming Lactobacillus: new challenges for the development of probiotics. Microorganisms, 4(3), 35.
Shariati, A., Asadian, E., Fallah, F., Azimi, T., Hashemi, A., Sharahi, J. Y., and Moghadam, M. T. (2019). Evaluation of Nano-curcumin effects on expression levels of virulence genes and biofilm production of multidrug-resistant Pseudomonas aeruginosa isolated from burn wound infections in Tehran, Iran. Infection and Drug Resistance, 12, 2223.
Sharifi-Rad, J., Rodrigues, C. F., Stojanović-Radić, Z., Dimitrijević, M., Aleksić, A., Neffe-Skocińska, K., ... and Calina, D. (2020). Probiotics: versatile bioactive components in promoting human health. Medicina, 56(9), 433.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864
Vallor, A. C., Antonio, M. A., Hawes, S. E., and Hillier, S. L. (2001). Factors associated with acquisition of, or persistent colonization by, vaginal lactobacilli: role of hydrogen peroxide production. The Journal of infectious diseases, 184(11), 1431-1436.
Vélez, M. P., Hermans, K., Verhoeven, T. L. A., Lebeer, S. E., Vanderleyden, J., and De Keersmaecker, S. C. J. (2007). Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. Journal of Applied Microbiology, 103(3), 666-674.
Vindenes, H., and Bjerknes, R. (1995). Microbial colonization of large wounds. Burns, 21(8), 575-579.
Wasfi, R., Mansour, L., Hashem, A., Abd El-Rahman, O., Hanora, A., and Ashour, M. (2012). Antimicrobial activities against biofilm formed by Proteus mirabilis isolates from wound and urinary tract infections. Indian Journal of Medical Microbiology, 30(1), 76.
Wasfi, R., Mansour, L., Hashem, A., Abd El-Rahman, O., Hanora, A., and Ashour, M. (2012). Antimicrobial activities against biofilm formed by Proteus mirabilis isolates from wound and urinary tract infections. Indian Journal of Medical Microbiology, 30(1), 76.
Wu, X. D., Liu, M. M., Liang, X., Hu, N., and Huang, W. (2018). Effects of perioperative supplementation with pro-/synbiotics on clinical outcomes in surgical patients: a meta-analysis with trial sequential analysis of randomized controlled trials. Clinical Nutrition, 37(2), 505-515.
Wu, X. Y. (2006). Studies on the impact of probiotic bacteria on enteric microbial diversity and immune response.
Yu, R., Zhang, H., and Guo, B. (2022). Conductive biomaterials as bioactive wound dressing for wound healing and skin tissue engineering. Nano-micro letters, 14(1), 1-46.
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