Molecular detection of norovirus and TNF-α polymorphism in children with acute gastroenteritis infection in Samawa, Iraq

Sabreen Fa. Hassan; Noor Sami AL-Lebawy

doi:10.53730/ijhs.v6nS8.12276

Authors

Sabreen Fa. Hassan Department of Biology, College of Science, Al Muthanna University, Iraq
Noor Sami AL-Lebawy
nwrn22302@gmail.com
Department of Biology, College of Science, Al Muthanna University, Iraq

Keywords:

acute gastroenteritis, human noroviruses GI, human noroviruses GII, real time–PCR, TNF-α, SNPs

Abstract

Human Norovirus is the leading cause of non-bacterial gastroenteritis in children worldwide, and research into it is critical for outbreak prevention and infection control. The multiplex real time PCR technique was evaluated in this study using 100 specimens (stool and blood) collected from children in Al-Muthanna province. RT-Qpcr for NVGI and NVGII was used to test all samples for Norovirus. The overall incidence rate of human Norovirus infection was 26%, with the first genogroup G1 accounting for only one case (2%) and the second genogroup accounting for 12 cases (24%). The findings revealed a high prevalence of previous virus infection in the age group (1-10) months. Infections occurred equally among males and females, with no significant difference in infection rates; females were infected at a rate of 42%, while males were infected at a rate of 58%. Males were infected at a higher rate (53.8%) than females (46.2%). The results of Tetra ARMS -PCR after DNA extraction for patients and amplification using the previous method show that there are three genotypes for G/G among patients compared to two results for A/A in the control groups. As a result, association and both homozygous as risk factors for HNV infection development.

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