Spectrophotometric method for the determination of chlordiazepoxide, diazepam and alprazolam drugs by using oxidation coupling reaction
Keywords:
chlordiazepoxide, Diazepam and Alprazolam, MBTH, Oxidative coupling reactionAbstract
Sensitive, accurate, precise and efficient spectrophotometric"method for the determination of chlordiazepoxide, Diazepam and Alprazolam drug (CH, DIA, ALP) in pure as well as in dosage form was described."The suggested method was based on oxidation coupling reaction of suggested drug (CH, DIA, ALP) drug using acidic buffer "then"coupling with"3-methyl-2-benzothiazolinone hydrazone hydrochloride"(MBTH) in the presence of ferric chloride. Spectrophotometric measurement was established by recording the absorbance of the blue colored product at (663,609,619) nm. Using the optimized reaction conditions, beer’ s law was obeyed in the range of"(2.5-25) µg/mL "(5-30) µg/mL, and "(5-30) µg/mL with good correlation coefficient of (0.9997, 0.9979, 0.9965) and the LOD and LOQ ( CH,DIA,ALP) values were( 0.1750, 5.834 , 0.676 and 2.253 ,0.881and 2.938)μg/mL for chlordiazepoxide, Diazepam and Alprazolam drug respectively. The accuracy and precision of the proposed method represented by recovery and relative standard deviation were satisfactory; about 99.33% and)") 0.502and 0.195, 0.46) μg/mL for chlordiazepoxide, Diazepam and Alprazolam drug respectively. The "proposed"method"was"applied"for"determination"of (CH, DIA, ALP) in"its"pharmaceutical" "forms"and"the"results"compared successfully"with"the"obtained"by"standard"method (British"pharmacopeia"method).
Downloads
References
Abdulbari, M. M., & Ihsan, M. S. (2013). Simultaneous determination and validation of chlorpheniramine maleate, acetaminophen, phenylpropanolamine hydrochloride and caffeine in tablet dosage form by using reverse phase high performance liquid chromatography (RP-HPLC). Int. J. Pharm. Sci, 5, 666-670.
Aditama, A. P. R., Ma’arif, B., Laswati, H., & Agil, M. (2022). The effect of ethyl acetate fraction of Marsilea crenata presl. leaves in increasing osterix expression in hFOB 1.19 cells. International Journal of Health Sciences, 6(2), 789–796. https://doi.org/10.53730/ijhs.v6n2.8096
Ali, A., Ahmed, M., Mahmud, T., Qadir, M. A., Nadeem, K., & Saleem, A. (2015). Stability-indicating High-performance liquid chromatography method for simultaneous determination of aminophylline and chlorpheniramine maleate in pharmaceutical formulations. Indian journal of pharmaceutical sciences, 77(5), 515.
Ali, F. A. R. A. A. T., Nandi, U., Verma, R., Rathod, R., Sahu, P. L., Kumar, R., ... & Singh, G. N. (2016). UV-visible first order derivative spectrophotometric method development and validation for simultaneous estimation of amitriptyline hydrochloride and chlordiazepoxide in tablet dosage form. Asian Journal of Chemistry, 28(12), 2632-2634.
Ali, N., Abdelkawy, M., & Abdelhamid, N. S. (2013). Simultaneous determination of paracetamol and diphenhydramine hydrochloride mixture in the presence of their degradation products. IOSR J Pharm Biol Sci, 6(5), 44-52.
Atta, N. F., Galal, A., & Azab, S. M. (2011). Electrochemical determination of paracetamol using gold nanoparticles–application in tablets and human fluids. Int. J. Electrochem. Sci, 6, 5082-5096.
Atto, R. A. (2012). New method for determination of Diclofenac sodium by High Performance Liquid Chromatography. Tikrit J Pharma Sci, 8(1), 60-67.
Bertolasi, V., Sacerdoti, M., Gilli, G., & Borea, P. A. (1982). Structure of 7-chloro-2-methylamino-5-phenyl-3H-1, 4-benzodiazepine 4-oxide (chlordiazepoxide). Acta Crystallographica Section B: Structural Crystallography and Crystal Chemistry, 38(6), 1768-1772.
Chandra, R., & Sharma, K. D. (2013). Quantitative determination of paracetamol and caffeine from formulated tablets by reversed phase-HPLC separation technique. Int J Chromatogr Sci, 3(2), 31-34.
Darwish, H. W., Metwally, F. H., & El Bayoumi, A. (2015). Development of three methods for simultaneous quantitative determination of chlorpheniramine maleate and dexamethasone in the presence of parabens in oral liquids. Tropical Journal of Pharmaceutical Research, 14(1), 153-161.
Farid, N. F., El Ragehy, N. A., Hegazy, M. A., Abdelkawy, M., & Metwally, F. H. (2014). Simultaneous determination of a quaternary mixture of oxomemazine, sodium benzoate, guaifenesin and paracetamol by chromatographic methods. Beni-Suef University Journal of Basic and Applied Sciences, 3(4), 260-268.
KHAN, N. A., Naz, N., KHAN, N. A., DOGER, N. A., & KHAN, W. A. (2012). Spectrophotometric Determination of Diazepam. Journal of the Chemical Society of Pakistan, 34(6), 536.
Klotz, U., Antonin, K. H., & Bieck, P. R. (1976). Pharmacokinetics and plasma binding of diazepam in man, dog, rabbit, guinea pig and rat. Journal of Pharmacology and Experimental Therapeutics, 199(1), 67-73.
Kumar, S. A., Debnath, M., & Vimala, D. (2016). Simultaneous estimation of phenylephrine HCl, chlorpheniramine maleate and dextromethorphan HBr in a pharmaceutical (syrup) formulations by RP-HPLC using PDA detector. Pak. J. Pharm. Res, 2(02), 89-97.
M Mahood, A. (2017). RP-HPLC Method for Simultaneous Estimation of Diclofenac sodium, Chlorphenaramine malate and Paracetamol in Tablets. karbala journal of pharmaceutical sciences, 8(12), 15-28.
Moyano, M. A., Rosasco, M. A., Pizzorno, M. T., & Segall, A. I. (2005). Simultaneous determination of chlorpheniramine maleate and dexamethasone in a tablet dosage form by liquid chromatography. Journal of AOAC International, 88(6), 1677-1683.
Patel, P. N., Samanthula, G., Shrigod, V., Modh, S. C., & Chaudhari, J. R. (2013). RP-HPLC method for determination of several NSAIDs and their combination drugs. Chromatography Research International, 2013.
Patel, S. K., & Patel, N. J. (2009). Simultaneous RP-HPLC estimation of trifluoperazine hydrochloride and chlordiazepoxide in tablet dosage forms. Indian Journal of Pharmaceutical Sciences, 71(5), 545.
Perez, A. V., Briones, V. V., Viteri, C. G. V., & Gamez, M. R. (2017). Iberoamerica in network, GIS & TIC. International Journal of Social Sciences and Humanities, 1(3), 108–117. https://doi.org/10.29332/ijssh.v1n3.63
Pharmacopoeia, I. (1996). Govt. of India, ministry of health and family welfare, Delhi. Publication by Controller of Publication, 2, 484-554.
Rajan, R., & Deshpand, A. (2016). UV spectrophotometric estimation of alprazolam by area under curve and first order derivative methods in bulk and pharmaceutical dosage form. Der Pharmacia Lettre, 8(5).
Redasani, V. K., Gorle, A. P., Badhan, R. A., Jain, P. S., & Surana, S. J. (2013). Simultaneous determination of chlorpheniramine maleate, phenylephrine hydrochloride, paracetamol and caffeine in pharmaceutical preparation by RP-HPLC. Chemical Industry and Chemical Engineering Quarterly/CICEQ, 19(1), 57-65.
Redasani, V. K., Gorle, A. P., Badhan, R. A., Jain, P. S., & Surana, S. J. (2013). Simultaneous determination of chlorpheniramine maleate, phenylephrine hydrochloride, paracetamol and caffeine in pharmaceutical preparation by RP-HPLC. Chemical Industry and Chemical Engineering Quarterly/CICEQ, 19(1), 57-65.
Saudagar, R. B., Saraf, S., & Saraf, S. (2007). Spectrophotometric determination of chlordiazepoxide and trifluoperazine hydrochloride from combined dosage form. Indian journal of pharmaceutical sciences, 69(1), 149.
Shi, J. Y., Liu, N., Gu, R. X., Zhu, L. Q., Zhang, C., Wang, Q. G., ... & Ren, J. Y. (2015). Signals induced by exogenous nitric oxide and their role in controlling brown rot disease caused by Monilinia fructicola in postharvest peach fruit. Journal of general plant pathology, 81(1), 68-76.
Shukla, S., Kumar, P., Srivastava, R. S., & Trivedi, P. (2010). RP-HPLC method development and its validation for simultaneous estimation of Alprazolam and Fluoxetine hydrochloride in pharmaceutical dosage form. Eurasian Journal of Analytical Chemistry, 5(3), 239-245.
Sornchaithawatwong, C., Vorrarat, S., & Nunthanavanit, P. (2010). Simultaneous determination of paracetamol and its main degradation product in generic paracetamol tablets using reverse-phase HPLC. Journal of Health Research, 24(3), 103-106.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938
Vemula, V. R. B., & Sharma, P. K. (2013). Analytical method development and validation for simultaneous estimation of imipramine and diazepam in tablet dosage form by RP-HPLC. Int J Pharm Pharm Sci, 5, 249-253.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
