The clinical and histopathological study, the effects of platelet-rich fibrin on skin pedicle graft healing
Keywords:
Goats, pedicle graft, PRF, Skin, HistopathologyAbstract
Background: Tissue engineering aims to repair or replace damaged tissue in response to various injury circumstances. One of these sectors is platelet-rich fibrin (PRF), extensively employed for soft tissue regeneration in recent years. Objective: The research aimed to examine the clinical and histological effects of allogenic PRF on skin pedicle grafts used to treat experimental avulsion wounds. Materials and method: Fifteen adult female goats were used for this study. Under heavy sedation and complete aseptic conditions, 2 skin marks (2×2 cm) were created on both sides of the trunk region of the animal. Two total thicknesses of the blotchy skin are cut carefully. Advancement (sliding) flaps (2×6 cm) are harvested parallel to the lines of most minor tension and slid over the adjacent wound. These wounds are allocated into two groups, the first one as the control group and the second group treated with autogenic platelet-rich fibrin. Each goat has two groups represented. The edges of the wounds were closed by 2-0 nylon monofilament sutures with a simple interrupted pattern. Results: Clinical examination showed the amount of wound exudation in the treated group significantly increased compared to the control group.
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• Abdulrazaq, W. A. (2012). Early Eventin Effect of Apple Cidar Vinegar on The Induced Burn Healing in Rabbits. Bas. J. Vet. Res., Vol. 11, No. 2, pp: 17-27.
• Ahmed, Jihad & Bannai, Jinan & Abdulsamad, Rana. (2017). Topical Application to Mixture of Honey with Cod Liver Oil as a Novel Therapy to Treat Contaminated Skin Wounds in Rabbits Model. 2017.
• Alfars, A. A. (2009) Histological Study of Early Events During Wound Healing in Rabbits. Bas. J. Vet. Res. Vol. 8, No. 1. Pp: 130-136.
• Bosanquet, D. C., Rangaraj, A., & Richards, A. J., Riddell, A., Saravolac, V. M. & Harding, K. G. (2013). Topical steroids for chronic wounds displaying abnormal inflammation. Ann R Coll Surg Engl, Vol. 95, Issue 6, pp : 291–296.
• Broughton, G., Janis, J. & Attinger, C. (2006). The basic science of wound healing. Plast Reconstr Surg, 117:12S.
• Browne, E. Z. (1986). Complications of Skin Grafts and Pedicle Flaps Hand Clinics. Volume 2, Issue 2, pp: 353-359.
• Cutting, K. F., Vowden, P. & Wiegand, C. (2015). Wound inflammation and the role of a multifunctional polymeric dressing. Wounds International, Vol 6, Issue 2, pp: 41-46.
• Desai, C. B., Mahindra, U. R., Kini, Y. K. & Bakshi, M. K. (2013). Use of Platelet Rich Fibrin over Skin Wounds: Modified Secondary Intention Healing (CASE REPORT). Journal of Cutaneous and Aesthetic Surgery, Volume 6, Issue 1, pp: 35-37.
• Ding , Y., Cui, L., Zhao, Q., Zhang, W., Sun, H. & Zheng, L. ( 2017). Platelet-Rich Fibrin Accelerates Skin Wound Healing in Diabetic Mice. Ann Plast Surg. 79(3): e15-e19.
• Dohan, D. M., Choukroun, J., Diss, A., Dohan S. L., Dohan, A. J., Mouhyi, J. & Gogly, B. (2006). Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 101:E37-44.
• Gantwerker, E. A. & Hom, D. B. (2011). Skin: histology and physiology of wound healing. Facial Plast Surg Clin North Am. 19(3):441-53.
• Goldberg, S. R. & Diegelmann, R. F. (2010). Wound Healing Primer. Surg Clin N Am, 90 pp: 1133–1146.
• Hashim, A. M., Abd, H. H. & Hellal, M. M. (2021). Histopathology Study of the Platelet Rich Plasma on the Wound Healing in Rabbits. Medico-legal Update, Vol. 21, No. 2 pp: 1309- 1314.
• Khashjoori, B. K., Ghamsari, S. M., Dehghan, M. M., Mohajeri, S. F. Golshahi, H. (2019). Evaluation of the Effects of Adipose Derived Mesenchymal Stem Cells Cultured on Decellularized Amniotic Membrane in Wound Healing of Distal Part of the Limbs in Horse. Iranian Journal of Veterinary Medicine, Iran J Vet Med., Vol 13, No 1, pp: 11-25.
• Molnar, A., Magyar, Z., Nachmias , D. B., Mann , D., Szabo , B., Toth , Z. & Nemeth, N. (2019). Effect of short-term ischemia on microcirculation and wound healing of adipocutaneous flaps in the rat. Acta Cir Bras., 34(12):e201901203
• Naeem, L. A., Abdul Kareem, Z. B., Jassim, M. M., Khalaf, A. A. & Jabbar, Z. A. (2021). Investigation on the Influence of Synovial Fluid and Vitreous Humour on Avulsion Wounds Healing in Rabbits. Archives of Razi Institute, Vol. 76, No. 4, pp: 1483-1491.
• Nichols, E. (2016). Wound assessment part 2: exudates. Wound Essentials, vol 11 no 1, pp: 36-41.
• Rodrigues, M., Kosaric, N., Bonham, C. A. & Gurtner G. C. (2019). Wound Healing: a Cellular Perspective, Physiol Rev, 99: 665-706.
• Soares, C. S., Dias, I. R., Pires, M. A. & Carvalho P. P. (2021). Canine-Origin Platelet-Rich Fibrin as an Effective Biomaterial for Wound Healing in Domestic Cats: A Preliminary Study. Vet Sci. 8(10): 213.
• Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938
• Soares, C. S., Dias, I. R., Pires, M. A. & Carvalho, P. P. (2016). Effects of Autologous Platelet-Rich Fibrin (PRF) Therapy on Wound Healing in Dogs. Research Square, 23 pages.
• Velnar, T., Bailey,T. & Smrkolj, V. (2009).The Wound Healing Process: an Overview of the Cellular and Molecular Mechanisms. J Int Med Res 37(5): 1528 – 1542.
• Zhang , L., Liu, L., Zhang, J. & Zhou, P. (2022). Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats. Hindawi, Evidence-Based Complementary and Alternative Medicine, Volume 2022, Article ID 5063625, 10 pages
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