Schiff base method characterization of the newly synthesized Modified Levofloxacin Complex (MOLVX) and its activity against the HRT-18 cell line isolated from a male patient with colorectal adenocarcinoma

Mohammed Khaleel Tarab; Asmaa Mohamed Noori Khaleel; Falah Muosa Kadhim Al-Rekabi

doi:10.53730/ijhs.v6nS7.12689

Authors

Mohammed Khaleel Tarab Department of Clinical Pharmacology, College of Medicine, University of Kirkuk, Iraq, Kirkuk, Iraq
Asmaa Mohamed Noori Khaleel College of Science, Department of Chemistry, University of Baghdad, Iraq. Campus college of science, Baghdad, Iraq
Falah Muosa Kadhim Al-Rekabi Department of pharmacology and toxicology, College of Veterinary Medicine, University of Baghdad, Iraq, Alameria- Baghdad, Baghdad

Keywords:

HRT-18 cell line, Modified Levofloxacin Complex MOLVX, Colorectal Adenocarcinoma

Abstract

Complexes have been synthesized using 10mL methanolic solution of raw levofloxacin (C₁₈H20FN₃O₄) (0.0144g, 0.2mmol), 5mL methanolic solution of copper chloride dihydrate (II) (CuCl₂_2H₂O) (0.068g, 0.17mmol,), and 20mL methanolic solution of 1,10-phenanthroline(C₁₂H₈N₂) (132mg, 0.2mmol). The complex was characterized by the Schiff base method for detection of the modification of the structure of levofloxacin using Hydrogen-Nuclear Magnetic Resonance H-NMR, Fourier Transform Infrared Spectroscopy (FT-IR), UV–visible (UV–vis) spectrophotometry, and elemental analysis of the complex using CHN analysis. The dried precipitate appears as light green crystals which are ground well by a clean spatula and weighed. Yield: 340 mg; 99%. This complex and levofloxacin have been subjected to melting point checking which was 268-270, and 221℃ respectively. Elemental analyses were achieved and were calculated for [Cu (Levo.) (Phen)Cl]. Cl, (C₃₀H₃₃Cl₂CuFN₅O₄), molecular weight = 681.07 g/mol−1: C,53.29; H, 4.14; N, 10.36%.

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