Immunohistochemical evaluation of tumor angiogenesis and architectural changes in regional lymph nodes of oral squamous cell carcinoma (OSCC)

Thirumalaraju Kalyani; Yogesh Yadav; Anju Lata Rai; Devicharan Shetty; Nisha Kaul

doi:10.53730/ijhs.v6nS9.12695

Authors

Thirumalaraju Kalyani PhD Scholar, Department of Anatomy, Santosh Medical College Deemed to be University, Ghaziabad, UP
Yogesh Yadav HOD & Professor, Department of Anatomy, Noida International Institute of Medical Sciences, Greater Noida, UP
Anju Lata Rai
anjulata72@gmail.com
Professor, Department of Anatomy, ABVIMS, RML Hospital, New Delhi
Devicharan Shetty Dean, HOD & Professor, Department of Oral Pathology, ITS CDSR, Muradnagar, Delhi Meerut Road, Ghaziabad, UP
Nisha Kaul HOD & Professor, Department of Anatomy, Santosh Medical College, Deemed to be University, Ghaziabad

Keywords:

Oral Squamous Cell Carcinoma, Germinal center Predominance, Lymphocyte predominance, Mean vascular area, Tumor node metastasis

Abstract

Aim: To evaluate the mean vascular area (MVA) and to correlate it with immunomorphological pattern of lymph nodes. Materials and Methods: A total of 50 positive OSCC cases (clinically and histopathologically diagnosed) from Santosh Medical College and I.T.S Dental college, ghaziabad were taken for the study. The lymph nodes collected were stained with routine hematoxylin & eosin and accessed for immunomorphological pattern. The sections were evaluated immunohistochemically by anti-von Willebrand factor (vWF) VIII to analyze the micro vessels in OSCC. Results: Three hundred and forty two lymph nodes were evaluated and the most predominant lymph node patterns were found to be Germinal Center Predominance (37.43%). Our results showed a significant association between tumor node metastasis (TNM) staging, histopathological grading, and mean vascular area with lymph node reactivity pattern. Conclusion: There was an increase in angiogenesis with in different grades of OSCC and mean vascular area with morphological pattern of lymph nodes can be used as an additional parameter which would help in assessing prognosis and survival of the patient.

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