The effect of nonnutritive sweeteners on the antifungal activity of black and green tea aqueous extracts against salivary Candida albicans an (in-vitro study)

https://doi.org/10.53730/ijhs.v6nS7.12792

Authors

  • Dalya M. AL-Qaralusi Master student, Department of basic sciences, College of Dentistry, University of Baghdad/Iraq
  • Abbas S. Al-Mizraqchi PhD, Medical microbiology, Department of basic sciences, College of Dentistry, University of Baghdad

Keywords:

Candida albicans, Tea extracts, Antifungal activity, Stevia, Sucralose

Abstract

Oral diseases, while largely preventable, pose a major health burden for many countries and affect people throughout their lifetime, causing pain, discomfort and disfigurement. Mouth washing agents are an effective vehicle for antimicrobial compounds in reducing oral pathogens, managing dental plaque and maintaining proper oral health. Numerous plant extracts have been successfully incorporated into oral care products due to their antimicrobial properties, availability, and affordability. Tea plants and extracts are one of the most important natural compounds used in dental care products. The problem with tea extracts is their bitter taste, which could compromise their usage by people. So, it needs a kind of supplementation to be palatable, especially for the pediatric and geriatric populations. Hence a nonnutritive sweetener was added to tea extracts to suppress their bitter taste. This study aimed to determine the effect of nonnutritive sweeteners on the antifungal activity of black and green tea aqueous extracts against salivary C. albicans. The results showed that adding 1% of stevia and sucralose to the black and green tea aqueous extracts did not affect the antifungal activity against salivary C. 

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References

Ahmad, J., Khan, I., Blundell, R., Azzopardi, J., & Mahomoodally, M. (2020). Stevia rebaudiana Bertoni.: an updated review of its health benefits, industrial applications and safety. Trends In Food Science &Amp; Technology, 100, 177-189. https://doi.org/10.1016/j.tifs.2020.04.030

Al-Ezzi, M., Al-Mizraqchi, A., Al-Waheb, A., Bayasan, A., Seoudi, N. and Tappuni, A. (2018) Black and green tea antimicrobial effect on Mutans streptococci and Lactobacilli. JODR., 5(1), 36-54.

Al-Lamy, H. and Al-Mizraqchi, A. (2012) Antibacterial efficiency of salvia officinalis extracts and their effect on growth, adherence and acid production of oral Mutans Streptococci. J Bagh Coll Dentistry 24(sp. Issue 1):153-157.

AL-Mizraqchi, A. (1998) Adherence of mutans streptococci on the teeth surfaces: microbiological and biochemical studies (Doctoral dissertation, Ph. D. thesis)

Aldhaher Z. (2013) Antimicrobial activity of different types of mouthwashes against Streptococcus mutans, Staphylococcus aureus and Candida albicans (In vitro study). J Bagh. College Dentistry.25(2) :185-191.

Arendrup, M., Andersen, J., Holten, M., Krarup, K., Reiter, N., Schierbeck, J., & Helleberg, M. (2019). Diagnostic Performance of T2Candida Among ICU Patients with Risk Factors for Invasive Candidiasis. Open Forum Infectious Diseases, 6(5). https://doi.org/10.1093/ofid/ofz136

Atriwal, T., Azeem, K., Husain, F., Hussain, A., Khan, M., Alajmi, M., & Abid, M. (2021). Mechanistic Understanding of Candida albicans Biofilm Formation and Approaches for Its Inhibition. Frontiers In Microbiology, 12. https://doi.org/10.3389/fmicb.638609

Bark, K., Yeom, J., Yang, J., Yang, I., Park, C., & Park, H. (2011). Spectroscopic Studies on the Oxidation of Catechin in Aqueous Solution. Bulletin Of the Korean Chemical Society, 32(9), 3443-3447. https://doi.org/10.5012/bkcs.32.9.3443

Ben Abid, Z. (2015). Lemon Juice Counteracts the Effect of Green Tea Decoction on Body Weight Gains, High Fat Diet Induced-Liver Steatosis, Total Antioxidant Status and Some Metabolic Parameters in Rats. International Journal of Nutrition and Food Sciences, 4(1), 1. https://doi.org/10.11648/j.ijnfs.0401.11

Byadarahally Raju, S., & Rajappa, S. (2011). Isolation and Identification of Candida from the Oral Cavity. ISRN Dentistry, 2011, 1-7. https://doi.org/10.5402/2011/487921

Chen, L., Wang, W., Zhang, J., Wang, W., Ni, D., & Jiang, H. (2020). Dehydroascorbic Acid Affects the Stability of Catechins by Forming Conjunctions. Molecules, 25(18), 4076. https://doi.org/10.3390/molecules25184076

Chen, S., Wang, C., Tsai, C., Yang, I., Luo, S., & Chuang, Y. (2021). Fermentation quality evaluation of tea by estimating total catechins and theanine using near-infrared spectroscopy. Vibrational Spectroscopy, 115, 103278. https://doi.org/10.1016/j.vibspec.2021.103278

Cowan, M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews, 12(4), 564-582. https://doi.org/10.1128/cmr.12.4.564

Ghaheri, M., Adibrad, E., Safavi, S., Kahrizi, D., Soroush, A., & Muhammadi, S. et al. (2018). Effects of life cycle and leaf’s location on gene expression and glycoside biosynthesis pathway in Stevia rebaudiana Bertoni. Cellular And Molecular Biology, 64(2), 17-22. https://doi.org/10.14715/cmb/2018.64.2.4

Janakiram, C., Venkitachalam, R., Fontelo, P., Iafolla, T., & Dye, B. (2020). Effectiveness of herbal oral care products in reducing dental plaque & gingivitis – a systematic review and meta-analysis. BMC Complementary Medicine and Therapies, 20(1). https://doi.org/10.1186/s12906-020-2812-1

Kaur, S., & Kour, K. (2020). Short term side effects of 0.2% and 0.12% chlorhexidine mouthwash. IP International Journal of Periodontology and Implantology, 4(4), 138-140. https://doi.org/10.18231/j.ijpi.2019.029

Kesinger, N., & Stevens, J. (2009). Covalent interaction of ascorbic acid with natural products. Phytochemistry, 70(17-18), 1930-1939. https://doi.org/10.1016/j.phytochem.2009.09.028

Korir, M., Wachira, F., Wanyoko, J., Ngure, R., & Khalid, R. (2014). The fortification of tea with sweeteners and milk and its effect on in vitro antioxidant potential of tea product and glutathione levels in an animal model. Food Chemistry, 145, 145-153. https://doi.org/10.1016/j.foodchem.2013.08.016

Lohse, M., Gulati, M., Johnson, A., & Nobile, C. (2017). Development and regulation of single- and multi-species Candida albicans biofilms. Nature Reviews Microbiology, 16(1), 19-31. https://doi.org/10.1038/nrmicro.2017.107

Mageed, M., Saliem, S., Alwatar, A. (2015) Antibacterial effects of green tea extracts on Aggregatibacter actinomycetemcomitans (In-Vitro study). J Bagh Coll Dent. Sep. 14 [cited 2022 Aug. 26];27(3):102-8. Available from: https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/814

Martyn, D., Darch, M., Roberts, A., Lee, H., Yaqiong Tian, T., Kaburagi, N., & Belmar, P. (2018). Low-/No-Calorie Sweeteners: A Review of Global Intakes. Nutrients, 10(3), 357. https://doi.org/10.3390/nu10030357

Mlambo, R., Wang, J., & Chen, C. (2022). Stevia rebaudiana, a Versatile Food Ingredient: The Chemical Composition and Medicinal Properties. Journal Of Nanomaterials, 2022, 1-12. https://doi.org/10.1155/2022/3573005

Murray, P., & Baron, E. (2007). Manual of clinical microbiology. ASM Press.

Naglik, J., König, A., Hube, B., & Gaffen, S. (2017). Candida albicans –epithelial interactions and induction of mucosal innate immunity. Current Opinion in Microbiology, 40, 104-112. https://doi.org/10.1016/j.mib.2017.10.030

Paiva, L., Rego, C., Lima, E., Marcone, M., & Baptista, J. (2021). Comparative Analysis of the Polyphenols, Caffeine, and Antioxidant Activities of Green Tea, White Tea, and Flowers from Azorean Camellia sinensis Varieties Affected by Different Harvested and Processing Conditions. Antioxidants, 10(2), 183. https://doi.org/10.3390/antiox10020183

Pappas, P., Lionakis, M., Arendrup, M., Ostrosky-Zeichner, L., & Kullberg, B. (2018). Invasive candidiasis. Nature Reviews Disease Primers, 4(1). https://doi.org/10.1038/nrdp.2018.26

Purohit, V., & Mishra, S. (2018). The truth about artificial sweeteners – Are they good for diabetics? Indian Heart Journal, 70(1), 197-199. https://doi.org/10.1016/j.ihj.2018.01.020

Qanbar, F. and Al-Mizraqchi, A. (2009) The antimicrobial effect of aqueous and alcoholic extracts of eucalyptus leaves on oral Mutans streptococci, Lactobacilli and Candida albicans (an in vitro study). J Bagh. Coll Dentistry .21(4):109-12.

Raju, R., Bharadwaj, A., Byatappa, V., Umakanth, R., & Alayadan, P. (2020). Antiplaque and Antigingivitis Effectiveness of Tea Tree Oil and Chlorine Dioxide Mouthwashes among Young Adults: A Randomized Controlled Trial. World Journal of Dentistry, 11(6), 451-456. https://doi.org/10.5005/jp-journals-10015-1765

Samaranayake, L. (2018). Essential Microbiology for Dentistry -5th ed Philadelphia: Elsevier.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Schiano, C., Grimaldi, V., Scognamiglio, M., Costa, D., Soricelli, A., Nicoletti, G., & Napoli, C. (2021). Soft drinks and sweeteners intake: Possible contribution to the development of metabolic syndrome and cardiovascular diseases. Beneficial or detrimental action of alternative sweeteners? Food Research International, 142, 110220. https://doi.org/10.1016/j.foodres.2021.110220

Shalaby, E., Mahmoud, G., & Shanab, S. (2016). Suggested mechanism for the effect of sweeteners on radical scavenging activity of phenolic compounds in black and green tea. Frontiers In Life Science, 9(4), 241-251. https://doi.org/10.1080/21553769.2016.1233909

Shang, A., Li, J., Zhou, D., Gan, R., & Li, H. (2021). Molecular mechanisms underlying health benefits of tea compounds. Free Radical Biology and Medicine, 172, 181-200. https://doi.org/10.1016/j.freeradbiomed.2021.06.006

Spanemberg, J., Cardoso, J., Slob, E., & López-López, J. (2019). Quality of life related to oral health and its impact in adults. Journal Of Stomatology, Oral and Maxillofacial Surgery, 120(3), 234-239. https://doi.org/10.1016/j.jormas.2019.02.004

Sugano, N. (2012). Biological plaque control: novel therapeutic approach to periodontal disease. Journal Of Oral Science, 54(1), 1-5. https://doi.org/10.2334/josnusd.54.1

Tenovuo J, Lagerlöf F. Saliva. In: Textbook of clinical cariology. Second edition. Editors Thylstrup A and Fejerskov O. Pp. 17-43, chapter 2. Munksgaard, Copenhagen, Denmark, 1994

Tsui, C., Kong, E., & Jabra-Rizk, M. (2016). Pathogenesis of Candida albicans biofilm. Pathogens And Disease, 74(4), ftw018. https://doi.org/10.1093/femspd/ftw018

Valgas, C., Souza, S., Smânia, E., & Smânia Jr., A. (2007). Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology, 38(2), 369-380. https://doi.org/10.1590/s1517-83822007000200034

Willis, J., & Gabaldón, T. (2020). The Human Oral Microbiome in Health and Disease: From Sequences to Ecosystems. Microorganisms, 8(2), 308. https://doi.org/10.3390/microorganisms8020308

Wu, M., & Brown, A. (2021). Applications of Catechins in the Treatment of Bacterial Infections. Pathogens, 10(5), 546. https://doi.org/10.3390/pathogens10050546

Zhang, G., Yang, J., Cui, D., Zhao, D., Li, Y., Wan, X., & Zhao, J. (2020). Transcriptome and Metabolic Profiling Unveiled Roles of Peroxidases in Theaflavin Production in Black Tea Processing and Determination of Tea Processing Suitability. Journal Of Agricultural and Food Chemistry, 68(11), 3528-3538. https://doi.org/10.1021/acs.jafc.9b07737

Published

17-09-2022

How to Cite

AL-Qaralusi, D. M., & Al-Mizraqchi, A. S. (2022). The effect of nonnutritive sweeteners on the antifungal activity of black and green tea aqueous extracts against salivary Candida albicans an (in-vitro study). International Journal of Health Sciences, 6(S7), 4196–4207. https://doi.org/10.53730/ijhs.v6nS7.12792

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Section

Peer Review Articles