PCR detection of genes encoded antibiotic resistance in Stenotrophomonas maltophilia that isolated from clinical infections

Sura Qasssem Shaheed AL-Khafajy; Abbas S. J. ALMuhana; Jaafar Ahmed Abdulmunem Baqr AL-sham

doi:10.53730/ijhs.v6nS8.12841

Authors

Sura Qasssem Shaheed AL-Khafajy
255313sa@gmail.com
Faculty of Science, University of Kufa, Iraq
Abbas S. J. ALMuhana Faculty of Science, University of Kufa, Iraq
Jaafar Ahmed Abdulmunem Baqr AL-sham Faculty of Science, University of Kufa, Iraq

Keywords:

PCR detection, genes encoded antibiotic resistance, Stenotrophomonas maltophilia, clinical infections

Abstract

Background: Stenotrophomonas maltophilia is an aerobic, Gram-negative, no fermentative bacteria. It is an uncommon bacteria that is difficult to treat in people. The initial term was Bacterium bookeri, however it was eventually renamed to Pseudomonas maltophilia. It was resistant to multiple antibiotics, and its mechanisms also include acquired and intrinsic resistance. It is innately multi drug resistant (MDR) and found in watery and humid environments in the environment. Aim of study: The study amid to see if there were any antibiotic resistance genes in specimens that were found in S. maltophilia. Materials and methods: The S. maltophilia isolates were isolated and identified from 250 of clinical specimens, biochemically analyzed, susceptibility test by using Kirby-Bauer disk diffusion method and genetically screened for antibiotic resistance genes using a traditional (PCR). Results: The molecular profile revealed that (L1Q gene) was identified in 20 (100 %) of S. maltophilia isolates and (L2Q gene) was found in 20 (90 %) of S. maltophilia isolates for β- lactamase antibiotics, and (Aph gene) was found in 20 (15 %) of S. maltophilia isolates for aminoglycoside.

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