Feasibility of a nested PCR for the diagnosis of Trichomonas vaginalis infection in women a Wasit Province, Iraq
Keywords:
Tichomonas vaginalis, nested-PCR, human, microscopeAbstract
This study was concerned the describe of an current PCR method for specific detection of Tichomonas vaginalis DNA into a rapid nested PCR assay, and to estimate the recent prevalence of T. vaginalis according to studying different factors that effect on vaginal trichomoniasis in women complaining of vaginal discharge. Vaginal swab samples were collected from 100 patients lived at Wasit province, during the period from January, 2022 to July, 2022. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 100 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid nested PCR. All samples were examined by the direct microscopic examination (wet mount). The vaginal swabs examined by wet mount and nested PCR to detect the presence of T. vaginalis. Of the 100 women, 29 were positive by nested PCR giving the prevalence rate of (29%) with and the direct microscopic examination (wet mount) showed 4/100 (4 %) positive case. Their ages were different. Women aged (20-30)years had significantly higher prevalence of trichomoniasis (14%) than other age groups.
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