Isolation, Screening and molecular characterization of bio surfactant producing microbes from hydrocarbon polluted soil

Putha Deepika Sai Lakshmi; Beesa Hrushik Samyukth; Golamari Siva Reddy; Boddu Sumalatha

doi:10.53730/ijhs.v6nS9.12899

Authors

Putha Deepika Sai Lakshmi Department of Biotechnology, Koneru Lakshmaiah Education foundation, Green Fields, Vaddeswaram, Guntur, Andhrapradesh, India-522502
Beesa Hrushik Samyukth Department of Biotechnology, Koneru Lakshmaiah Education foundation, Green Fields, Vaddeswaram, Guntur, Andhrapradesh, India-522502
Golamari Siva Reddy
gsiva@kluniversity.in
Department of Biotechnology, Koneru Lakshmaiah Education foundation, Green Fields, Vaddeswaram, Guntur, Andhrapradesh, India-522502
Boddu Sumalatha Department of Chemical Engineering, Vignan’s Foundation for Science, Technology and Research, Guntur, Andhra Pradesh 522213, India

Keywords:

Alcaligenes sp., Bio surfactant, surface tension, emulsification index, drop collapse method, oil displacement method

Abstract

Biosurfactants are ampliphlic compounds which can reduce the surface and interfacial tension between the compounds and have multiuses in various industries and agriculture. The isolated lipopeptides and lipoprotein producing microbes are useful in removal of heavy metals from industrial effluents using bioremediation, pesticides and hydrocarbon contaminated sites. Lipopeptides and lipoproteins used as bio-control agent to protect plant against different diseases, leads in increases crop yield. The objective was to isolate potential bio surfactant producing microbes from hydrocarbons contaminated soil. Initially 21 microbes were isolated amidst them ten bio surfactant producers were screened based on the surface tension, emulsification index, drop collapse method and oil displacement method. The isolate KLEF21 was the most successful bio surfactant producer as it showed the minimum surface tension (32.12 dyne/cm) with an emulsification index of 68.97% and oil displacement activity 3.5 mm. The microbe was identified based on the partial sequence of 16S rRNA and it belongs to Alcaligenes and was indicated as Alcaligenes sp.KLEF21.The stain was also competent of employ polycyclic aromatic hydrocarbon composed of three fused benzene rings as the highest microbes biomass was produced by this strain in polycyclic aromatic hydrocarbon alter MSM media compared to other isolates.

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