Biosynthesis of gold Nanoparticles by Lyngbya sp. Clone Zen / Mira 16S ribosomal RNA gene and its antibacterial activity

https://doi.org/10.53730/ijhs.v6nS6.12920

Authors

  • Zena Yehea Kassim Al-Hasso Northern Technical University /Mosul Technical Institute Anesthesia Techniques Department
  • Mira Ausama Al-Katib University of Mosul / College of Education / Department of biology
  • Ghada Younis Abd Alrahman University of Mosul / College of dentistry / Department of dental basic sciences

Keywords:

biosynthesis, gold nanoparticles, ribosomal RNA gene, antibacterial

Abstract

Different methods to biosynthesis of Gold nanoparticles was done with using cyanobacterium Lyngbya sp. , and salt  gold  (HAuCL4) and evaluate their antibacterial activity .Gold nanoparticles were prepared in different ways ,dry and fresh weight of this cyanobacterium to obtain aqueous extract. The synthesized nanoparticles were characterized using analytical techniques such as UV-Visible spectroscopy, SEM (Scanning Electron Microscopy), FTIR (Fourier Transform Infrared) and XRD (X-Ray Diffraction). UV-visible spectroscopy show a Surface Plasmon Resonance (SPR) peak at (532- 552) nm; confirmed by (SEM) image revealed the spherical shape and the size range from (39.1-61.9) and (59.4-84.0) nm for dry, wet aqueous extract respectively. FTIR result showed containing of ( phenol ,protein and carbonyl) as afunctional group. The crystalline nature of the nanoparticles was evident from Peaks in the (XRD) pattern. biosynthesis

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References

A. Oren, “A proposal for further integration of the cyanobacteria under the Bacteriological Code,” Int. J. Syst. Evol. Microbiol., vol. 54, no. 5, pp. 1895– 1902, 2004 .

Abdel-Azeem, A.; Nada, A.A.; O’Donovan, A.; Kumar Thakur, V.; Elkelish, A. Mycogenic Silver Nanoparticles from Endophytic Trichoderma Atroviride with Antimicrobial Activity. J. Renew. Mater. 2019, 7, 171–185. [CrossRef].

Al-tamimi , Abdel Nasser Abdulla Mahdi (2019) . Book and lecturer on algae science . University of Mosul .

Anjana, P. M., Bindhu, M. R., Umadevi, M., & Rakhi, R. B. (2019). Antibacterial and electrochemical activities of silver, gold, and palladium nanoparticles dispersed amorphous carbon composites. Applied Surface Science, 479, 96-104.‏

Aref, M.S.; Salem, S.S. Bio-callus synthesis of silver nanoparticles, characterization, and antibacterial activities via Cinnamomum camphora callus culture. Biocatal. Agric. Biotechnol. 2020, 27, 101689. [CrossRef].

Bakir , M,Esam ; Nancy s. y ; Mohamed E.M. and El-Semary .A. (2018) . cyanobacteria as Nanogold factories : chemical and Anti-myocardial infraction properties of gold Nanoparticles synthesized by Lyngbya majuscule . Marine drugs . 16 ,217 , doi : 10.3390 /md 16060217.

Collenburg, L.; Beyersdorf, N.; Wiese, T.; Arenz, C.; Saied, E.M.; Becker-Flegler, K.A.; Schneider-Schaulies, S.; Avota, E. The Activity of the Neutral Sphingomyelinase Is Important in T Cell Recruitment and Directional Migration. Front. Immunol. 2017, 8, 1007. [CrossRef]

Ebadi ,M. ;Zolfagherari M.R. ; Aghaei ,S.S.;Zarger,M. ; Shafiei ,M.;Zahiri ,H.SH. and Noghabi K.A. (2019) A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation. RSC Advances . issue 41

Harborn ,J.B.(1984 ) . phytochemical methods Aguide to moclern techniques of plant analysis , 2nd edition London , Chapman and Hall .

Husain, S.; Afreen, S.; Yasin, D.; Afzal, B.; Fatma, T. Cyanobacteria as a bioreactor for synthesis of silver nanoparticles-an effect of different reaction conditions on the size of nanoparticles and their dye decolorization ability. J. Microbiol. Methods 2019, 162, 77–82. [CrossRef] [PubMed].

Lengke. M.; Fleet, M. and Southam, G. (2006). Morphology of gold nanoparticles synthesized by filamentous cyanobacteria from gold (I) Thiosulfate and gold (III)--- chloride complexes. Langmuir. 22(6): 2780- 7.doi:10.1021/La052652e

Moreno, R. (2012). Identification of algal strains by PCR amplification and evaluation of their fattyacid profiles for biodiesel production. LSU Master's Theses, 247.

Pessini , G.L.; Dias Filho ,B.P.; Nakamura , C.V. and Cortez,D.A.G.(2003). Antibacterial activity of extracts and neolignans from piper regneni.Var . Pallescens Yunck . Inst. Osaaldocruz ,Riode Janeire , 98 98):1115- 1120.

Rastogi, R.P.; Sinha, R.P. Biotechnological and industrial significance of cyanobacterial secondary metabolites. Biotechnol. Adv. 2009, 27, 521–539. [CrossRef]

Salehi ,b.;Mehrabian ,S ,and Sepahi , A.A. (2013) . The effect of cadmium oxide nanoparticles on Pseudomonas Aeruginosa Bacteria . Advanced studies in biology , Vol. 5 , no. 11,473-488 , HIKARI Ltd, www.m-hikari.com http://dx.doi.org/10.12988/asb.2013.31043

Samak, D.H.; El-Sayed, Y.S.; Shaheen, H.M.; El-Far, A.H.; Abd El-Hack, M.E.; Noreldin, A.E.; El-Naggar, K.; Abdelnour, S.A.; Saied, E.M.; El-Seedi, H.R.; et al. Developmental Toxicity of Carbon Nanoparticles during Embryogenesis in Chicken. Environ. Sci. Pollut. Res. 2020, 27, 19058–19072. [CrossRef] [PubMed].

Sathyavathi, R., Krishna, M. B., Rao, S. V., Saritha, R., & Rao, D. N. (2010). Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics. Advanced science letters, 3(2), 138-143.

Sirelkhatim , S. Mahmud , A. Seeni , N. H. M. Kaus , L. Ann , S. K. M. Bakhori , H. Hassan and D. Mohammad , Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism, Nano-Micro Lett., 2015, 7 , 219 —242

Srinath, B. S., & Rai, V. R. (2015). Biosynthesis of highly monodispersed, spherical gold nanoparticles of size 4–10 nm from spent cultures of Klebsiella pneumoniae. 3 Biotech, 5(5), 671-676

W. Braide, R. N. Nwaoguikpe, S. E. Oranusi, L. I. Udegbunam, C. Akobondu, and S. I. Okorondu, “The effect of biodeterioration on the nutritional composition and microbiology of an edible long-winged reproductive termite, Macroterms bellicosus. Smeathman,” Internet J. Food Saf., vol. 13, pp. 107–114, 2011.

Wehr , John D .and Sheath , Robert G. (2003) . Fresh water Algae of North America ,Ecology And Classification . Academic press .

Weidman, V. E.; Walne, P. R. and Tainor, F. R. (1984). A new technique for obtaining axinic culture of algae. Can. J. Bot., 42:985-995.

Published

22-09-2022

How to Cite

Al-Hasso, Z. Y. K., Al-Katib, M. A., & Alrahman, G. Y. A. (2022). Biosynthesis of gold Nanoparticles by Lyngbya sp. Clone Zen / Mira 16S ribosomal RNA gene and its antibacterial activity. International Journal of Health Sciences, 6(S6), 10810–10819. https://doi.org/10.53730/ijhs.v6nS6.12920

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Section

Peer Review Articles