Phytochemical and isolated compound speciocide from Kigelia africana fruit

R Jubilee; M Komala

doi:10.53730/ijhs.v6nS8.13020

Authors

Jubilee R Reasearch scholar, Department of Pharmacology, School of Pharmaceutical Sciences, Vels Institute of science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamil Nadu, India
Komala M
komala.sps@velsuniv.ac.in
Department of Pharmaceutics, School of pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamil Nadu, India

Keywords:

Kigelia, flavonoids, glycosides, specioside

Abstract

Phytochemicals produced by herbs and medicinal plants have a variety of therapeutic uses. They also help the plant's colour and organoleptic characteristics. Due to their absence of adverse effects, these phytochemicals are used in advanced medical systems to efficiently treat ailments. Because Kigelia Africana contains phytoconstituents, the plant has gained significant scientific attention for its wide range of pharmacological properties. It has been examined and shown to have a number of compounds that treat cancer, psoriasis, dysentery, inflammations, and bacterial infections. The goal of the current investigation was to identify and separate the chemical components found in the fruit extracts of Kigelia africana (Lam.) Benth. Preliminary phytochemical screening performed on n-hexane, chloroform, ethyl acetate, ethanol and aqueous extracts showed the presence of alkaloids, glycosides, phenols, flavonoids etc. The qualitative estimation of the phytoconstituents revealed the presence of total phenols, total flavonoids, total saponins and glycosides as 9.38, 2.64, 3.12 and 6.45 %w/w respectively in the ethanol extract. The ethanol extract was subjected to TLC and coloumn chromatography which resulted in 4 fractions out of which fraction F4 was in significant quantity. It was analyzed using HPTLC that showed the presence of one isolated molecule.

Downloads

Download data is not yet available.

References

Ahmed, Salman &Hasan, Mohtasheem. (2015). Standardization of crude drugs: A precise review. World Journal of Pharmaceutical Research. 4. 155-174.

Alejandro F. Barrero, Juan F. Sanchez, Antonio Barton, and Ignacio Rodriguez, Specionin and Speciosides A and B: New Aromatic Lactones from Ononisspeciosa, J. Nat. Prod. 1989, 52, 6, 1334–1337

Anonymous. The Indian pharmacopoeia;2nd ed. New Delhi, India: Govt. of India publication; 1966. pp. 33–4.

Avinash Kumar Reddy, G., TrilokMitra, M., Shilpa, T., Shabnam, S., SatishBabu, K., Jyothi M Joy. 2012. Variation of Phenols, Flavonoids and Antioxidant Potential in Various Parts of Foeniculumvulgare on Drying. International Journal of Chemical and Pharmaceutical Sciences. 3(1):74-79.

Barku, V.Y.A., Opoku-Boahen, Y., Owusu-Ansah, E. and Mensah, E.F., 2013. Antioxidant activity and the estimation of total phenolic and flavonoid contents of the root extract of Amaranthusspinosus.Asian Journal of Plant Science and Research, 2013, 3(1):69-74

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. 2nd ed. Delhi, India: Council of Scientific & Industrial Research; 1956. p. 173.

Christian Agyare, David DarkoObiri, Yaw DuahBoakye, Newman Osafo. 2013. 19 - Anti-Inflammatory and Analgesic Activities of African Medicinal Plants, Editor(s): Victor Kuete, Medicinal Plant Research in Africa, Elsevier, 725-752. https://doi.org/10.1016/B978-0-12-405927-6.00019-9.

Edeoga OH, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4:685–8.

J.B. Harborne, Methods of Extraction and Isolation, in: Phytochemical methods, third ed., Chapman and Hall, London, 1998, pp. 60-66.

John, B.I.J.U., Sulaiman, C.T., George, S. and Reddy, V.R.K., 2014. Total phenolics and flavonoids in selected medicinal plants from Kerala. International Journal of Pharmacy and Pharmaceutical Sciences, 6(1), pp.406-408.

Kemp, W., 1991. Infrared spectroscopy. In Organic Spectroscopy (pp. 19-99). Palgrave, London.

Khalid S, Shahzad A, Basharat N, Abubakar M, Anwar P (2018) Phytochemical Screening and Analysis of Selected Medicinal Plants in Gujrat. J PhytochemistryBiochem 2: 108.

Li, X.N., Hua, L.X., Sun, J., Ridge, C.D., Mazzola, E.P. and Chen, P., 2019. Assignment of 1H and 13C NMR data for iridoid glycoside derivatives. Magnetic Resonance in Chemistry, 57(4), pp.S117-S122.

Lin, L. J., Huang, X. B., &Lv, Z. C. (2016).Isolation and identification of flavonoids components from Pterisvittata L. SpringerPlus, 5(1), 1649.https://doi.org/10.1186/s40064-016-3308-9

Madaan, R., Bansal, G., Kumar, S. and Sharma, A., 2011. Estimation of total phenols and flavonoids in extracts of Actaeaspicata roots and antioxidant activity studies. Indian journal of pharmaceutical sciences, 73(6), p.666.

Osman AG, Ali Z, Chittiboyina AG, Khan IA. 2017. Kigeliaafricana fruit: Constituents, bioactivity, and reflection on composition disparities. World J Tradit Chin Med. 3:1-6.

Saini, Monika &Sangwan, Reetu& Khan, Mohammad & Kumar, Ashok &Verma, Ruchi& Jain, Sudha. (2019). Specioside (SS) &verminoside (VS) (Iridoid glycosides): isolation, characterization and comparable quantum chemical studies using density functional theory (DFT). Heliyon. 5. e01118. 10.1016/j.heliyon.2019.e01118.

Sethi, P.D., 1996. HPTLC: high performance thin-layer chromatography; quantitative analysis of pharmaceutical formulations. CBS publishers & distributors.

Williams, D.H. and Fleming, I., 1989. Infrared spectra. Spectroscopic methods in organic chemistry, 4, p.58.

Yadav R, Khare RK, Singhal A (2017) Qualitative Phytochemical Screening of Some Selected Medicinal Plants of Shivpuri District (MP). Int J Life SciScienti Res 3: 844-847.