A molecular analysis of Escherichia coli O157:H7 strains isolated from cow and buffalo milk by the use of PCR in Karbala province

Nada Gasid Shlash; Hikmat Al-Nassir; Ali H. Fadhil

doi:10.53730/ijhs.v6nS6.13112

Authors

Nada Gasid Shlash
na2810278@gmail.com
Department of Public Health, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq
Hikmat Al-Nassir Department of Public Health, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq
Ali H. Fadhil Department of Preventive and Internal Medicine, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq

Keywords:

Escherichia coli O157:H7, rfbO157 gene, fliCh7 gene, Kerbala

Abstract

This study was conducted at Karbala governorate through the months of November 2021 until March 2022. Total number of 240 sampling was carried out from street vender and local store (milk, yogurts and cheese) from cow and buffalo. These samples were collected from different locations in Karbala in the Iraqi province. Escherichia coli O157H7 serotype prevalence rate from cow and buffalo was the major focus of this study of the research was conducted. 120 cow products and 120 buffalo products with different Karbala regions (local shops and street vendors) consisting a total of 240 samples. After initial enrichment, samples were streaked on Chromagar TM E. coli O157:H7 and sorbitole Maconkey agar. Escherichia coli O157h7 isolates were serotyped for to be detectable of the o157 and h7 antigens non-sorbitol fermenting (NSF) Isolates. Additionally, the virulent factor was examined. (rfbO157 and fliCH7) genes by PCR procedures. 14 isolates from 120 Escherichia coli O157:H7 in cow 11% and 18 from 120 (15%) buffalo. All fourteen samples isolates from produced milk became observed to be positive (rfbO157andfliCH7) (100%) genes, but just 13 from 18 isolates from buffalo.

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