Detection of 16SrRNA gene Polymorphism using PCR for some bacterial species of clinical samples

Saif Saad Al-janabi

doi:10.53730/ijhs.v6nS9.13157

Authors

Saif Saad Al-janabi
saifsaad1989aljanbi@gmail.com
Medical Laboratory techniques department, Al-maarif University College, Iraq

Keywords:

Klebsiella pneumoniae, Escherichia coli, 16s rRNA gene, Transversion, Transition

Abstract

Fifty samples were collected from patients suffering from the presence of infections in the skin, including wounds and burns, from inside the Fallujah Teaching Hospital. The samples were collected in the period from September to December 2021, and after isolation, transplantation, and microscopic and molecular diagnosis, 30 samples were obtained on which the bacteria appeared Including (E. coli and K. pneumoniae), which constitute about 60 % of the total samples. The microscopic test showed its development on different culture media. These isolates were cultured on Chrom agar, MacConkey agar, and Blood agar. It was found that there was a variation of the species, where the highest percentage of bacteria (E. coli and K. pneumoniae) appeared, and biochemical tests were also used. By using the diagnosis of the 16srRNA gene to detect the variation between the isolates by the PCR technique. The results of the sequence analysis were examined by blast in the National Center for Biotechnology Information (NCBI) and BioEdit program to detect polymorphism.

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