Phytochemical evaluation of the marketed drakshasava formulation by spectroscopic & chromatographic methods

Ganesh B. Nigade; Meenakshi N. Deodhar; Rajashree S. Chavan

doi:10.53730/ijhs.v6nS9.13187

Authors

Ganesh B. Nigade
ganeshpharma2984@gmail.com
Assistant Professor, PDEA’s Seth Govind Raghunath Sable College of pharmacy, Saswad
Meenakshi N. Deodhar Principal, Lokmanya Tilak Institute of Pharmaceutical Sciences, Pune
Rajashree S. Chavan Principal, PDEA’s Seth Govind Raghunath Sable College of pharmacy, Saswad

Keywords:

Phytochemical, Drakshasava, spectroscopic, chromatographic

Abstract

Background: Drakshasava is a general health tonic, carminative and blood purifier. It is used in the treatment of anemia, digestive & cardiac disorders. A review of the literature suggested that modern analytical methods are not reported for the phytochemical evaluation of drakshasava. Objective: To report phytochemical evaluation of Drakshasava Formulation by Spectroscopic & Chromatographic Methods. Materials and methods: Total phenolic, flavonoid, sugar and reducing sugar content in marketed drakshasava formulations were estimated by a UV-Vis spectrophotometer. The markers and extract of drakshasava formulations I & II were scanned by FTIR spectrophotometer individually to find the common bands of the vibrational spectra of markers and formulations. The markers and extract of drakshasava formulations I & II were subjected to HPLC analysis. Results: UV-Vis spectrophotometer analysis revealed that total phenolic, flavonoid, total sugar, & total reducing sugar of marketed drakshasava formulations were within the limit of ayurvedic pharmacopeia. The gradient mobile phase solvent A (HPLC grade water containing 0.85% w/v ortho phosphoric acid) and solvent B (acetonitrile) were optimized by considering the resolution, peak shape, and symmetry. Conclusion: The evaluation parameters reported in this article could be used as a standard reference for quality management of marketed drakshasava formulations.

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