Comparative evaluation of chemiluminescence immunoassay and immunochromatograhic test with ELISA for detection of HCV infection
Keywords:
HCV- Hepatitis C virus, ELISA- Enzyme linked immunosorbent assay, CLIA- chemiluminescent immunoassay, ICT- immunochromatographic testAbstract
Introduction: Hepatitis C virus still remains the major concern of post-transfusion viral infection causing chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide. Serological detection of anti-HCV IgG antibody is the mainstay for diagnosis of HCV. Enzyme linked immunosorbent assay (ELISA) is one of the commonest method used. Other screening test include chemiluminescent immunoassay (CLIA) and immunochromatographic test (ICT). This was a prospective laboratory based study conducted with the aim to assess anti-HCV detection method like ICT, CLIA, and ELISA with reliable sensitivity and specificity. Material and methods: A total of 92 samples that were received for routine HCV antibody detection to the microbiology laboratory were subjected to CLIA, ELISA and ICT simultaneously taking Elisa as gold standard & comparative evaluation was done. Results: Out of 92 samples, CLIA showed 34 (37%) reactive and 58 (63%) non-reactive. Whereas, ELISA and ICT showed 30 (32.6%) positive and 62 (67.3%) negative results. Conclusion: The results of CLIA & ICT was comparable with results of ELISA. CLIA had a sensitivity and specificity of 96.6% and 91.9%, ICT had a sensitivity and specificity of 98.38% and 96.6% accordingly, when compared to ELISA.
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