Molecular diagnosis of Toxoplasma gondii in four species of birds in Al-Najaf Province

Haider M. A. Alkaabi; Jasim H. R. Alkhuzaie

doi:10.53730/ijhs.v6nS8.13214

Authors

Haider M. A. Alkaabi Biology Determent, Faculty of Education for Girls, University of Kufa, Iraq
Jasim H. R. Alkhuzaie
Jasim.alkhuzaie@uokufa.edu.iq
Altoosi University College

Keywords:

Toxoplasma gondii, Gallinula chloropus, Numida meleagris, Pterocles alchata, Alectoris chukar, birds, PCR

Abstract

The current study aimed to investigate the T. gondii in four species of birds, including Pin-tailed Sandgrouse (Pterocles alchata), Chukar partridge (Alectoris chukar), Common Moorhen (Gallinula chloropus), and Helmeted Guineafowl (Numida meleagris) in Al-Najaf Al-Ashraf province in Iraq for the period from April 2021 until the end of April 2022 by using the polymerase chain reaction (PCR) technique. To our knowledge, this study is the first in Iraq to investigate these parasites in these species of birds by using this technique. Gene 18S rRNA with molecular weight 590bp of T. gondii was used, 25 samples were collected for each species of target birds except for P. alchata, 40 samples. The results of testing 115 tissue samples from the birds included in the study indicated that there were 8 samples (6.95%) of birds that gave a positive test result, It included 4 samples (50%) of N. meleagris, 2 samples (25%) of A. chukar, 2 (25%) of G. chloropus, and no case of the presence of the gene 18S rRNA was recorded in the samples from P. alchata.

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