The impact of oxidative stress on the activity of adenosine deaminase and its isoenzymes in nephropathy patients from Wasit-Iraq

Hussein Faleih Hassan; Nada A. Kadhim; Murtada Ali Jassim

doi:10.53730/ijhs.v6nS8.13342

Authors

Hussein Faleih Hassan
hussein19921130@gmail.com
Department of Chemistry, College of Sciences, University of Baghdad, Iraq & Medical Laboratory Technique, KutUniversity College, Al-kut, Wasit, Iraq, 52001
Nada A. Kadhim Department of Chemistry, College of Sciences, University of Baghdad, Iraq
Murtada Ali Jassim Al-Zahraa Teaching Hospital (Wasit –Iraq)

Keywords:

diabetic nephropathy, ADA, ADA isoenzyme, oxidative stress

Abstract

Diabetes mellitus is a group of metabolic disorders that share with symptoms of hyperglycemia led to elevated free radical activity. Hyperglycemia is linked to a higher level of (ADA), which is one of the factors that cause oxidative stress by creating reactive oxygen species (ROS), that leads to insulin resistance. This study aimed to determine the relationship between serum total adenosine deaminase(TADA) and its isoenzyme (ADA1and ADA2) with the progression of nephropathy in patients with type 2 diabetes using a case-control study from Wasit-Iraq. In addition, we aimed to find if there is a link between oxidative stress with TADA and its isoenzyme. The One-way ANOVA was used to compare the mean values of fasting plasma sugar (FPS), total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), microalbumin in urine, HbA1c, and TADA, ADA1, ADA2 between the three studied groups (Control(C), diabetic patients(DM), and diabetic nephropathy patient(DN). The results of our study revealed that there was a highly significant increase in TADA and its isoenzyme activities of DM and DN groups, as compared to the C group.

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References

Ahmad, Irshad, and Muddasarul Hoda. 2020. “Attenuation of Diabetic Retinopathy and Neuropathy by Resveratrol: Review on Its Molecular Mechanisms of Action.” Life Sciences 245:117350.

Aktas, Gulali, Mehmet Zahid Kocak, Satilmis Bilgin, Burcin Meryem Atak, Tuba Taslamacioglu Duman, and Ozge Kurtkulagi. 2020. “Uric Acid to HDL Cholesterol Ratio Is a Strong Predictor of Diabetic Control in Men with Type 2 Diabetes Mellitus.” The Aging Male 23(5):1098–1102.

AL-Fayyadh, Mohanad S. 2022. “Effects of Lipid Peroxidation, Thyroid Hormones, and Some Vitamins in Type 2 Diabetic Patients.” Iraqi Journal of Science 508–16.

Alicic, Radica Z., Emily J. Johnson, and Katherine R. Tuttle. 2018. “Inflammatory Mechanisms as New Biomarkers and Therapeutic Targets for Diabetic Kidney Disease.” Advances in Chronic Kidney Disease 25(2):181–91.

Al-Musawi, Hawraa Sabah, MakarimQassim Al-Lami, and Ali H. Al-Saadi. 2021. “Assessment of Glycemic Control, Renal Function, and Oxidative Stress Parameters in Type 2 Diabetes MellitusPatients.” Iraqi Journal of Science 4628–38.

Anon. 2020. “Adenosine Deaminase- Relation with Diabetes and Its Complication.” 5–8. doi: 10.7860/NJLM/2020/45058.

Association, American Diabetes. 2014. “Diagnosis and Classification of Diabetes Mellitus.” Diabetes Care 37(Supplement 1):S81–90.

Bae, Eun Hui, Tae Ryom Oh, Sang Heon Suh, Eun Mi Yang, Hong Sang Choi, Chang Seong Kim, Seong Kwon Ma, Bongseong Kim, Kyung-Do Han, and Soo Wan Kim. 2022. “Underweight and Weight Change Increases End-Stage Renal Disease Risk in Patients with Diabetes: A Nationwide Population-Based Cohort Study.” Nutrients 14(1):154.

Domecq, Juan Pablo, Gabriela Prutsky, Tarig Elraiyah, Zhen Wang, Karen F. Mauck, Juan Pablo Brito, Chaitanya Undavalli, Vishnu Sundaresh, Larry J. Prokop, and Victor M. Montori. 2019. “Medications Affecting the Biochemical Conversion to Type 2 Diabetes: A Systematic Review and Meta-Analysis.” The Journal of Clinical Endocrinology & Metabolism 104(9):3986–95.

Erel, Ozcan. 2004. “A Novel Automated Method to Measure Total Antioxidant Response against Potent Free Radical Reactions.” Clinical Biochemistry 37(2):112–19.

Erel, Ozcan. 2005. “A New Automated Colorimetric Method for Measuring Total Oxidant Status.” Clinical Biochemistry 38(12):1103–11.

Ganjifrockwala, Farzana Anis, J. T. Joseph, and G. George. 2017. “Decreased Total Antioxidant Levels and Increased Oxidative Stress in South African Type 2 Diabetes Mellitus Patients.” Journal of Endocrinology, Metabolism and Diabetes of South Africa 22(2):21–25.

Garofalo, Carlo, Silvio Borrelli, Roberto Minutolo, Paolo Chiodini, Luca De Nicola, and Giuseppe Conte. 2017. “A Systematic Review and Meta-Analysis Suggests Obesity Predicts Onset of Chronic Kidney Disease in the General Population.” Kidney International 91(5):1224–35.

Gonçalves, Karina Costa Coelho, and Luciene de Carvalho Cardoso Weide. 2021. “Adenosine Deaminase and Its Role in Diabetes Mellitus Type 2: Possible Marker of Insulin Resistance?” EC Endocrinology and Metabolic Research 6:39–41.

Gowda, M. N. Vanitha, K. C. Vasudha, S. Reshma, and K. J. Sujatha. 2012. “Serum Adenosine Deaminase Activity in Type 2 Diabetes Mellitus Patients.” International Journal of Diabetes in Developing Countries 32(3):176–81.

Hamid, Ghada Salam, Ali A. Allawi, and Kadhim K. Ghudhaib. 2021. “Correlation of Pentosidine with Kidney Diseases in Iraqi Patients with Diabetic Nephropathy.” Iraqi Journal of Science 3436–42.

Hunter, M. I. S., B. C. Nlemadim, and D. L. W. Davidson. 1985. “Lipid Peroxidation Products and Antioxidant Proteins in Plasma and Cerebrospinal Fluid from Multiple Sclerosis Patients.” Neurochemical Research 10(12):1645–52.

Incalza, Maria Angela, Rossella D’Oria, Annalisa Natalicchio, Sebastio Perrini, Luigi Laviola, and Francesco Giorgino. 2018. “Oxidative Stress and Reactive Oxygen Species in Endothelial Dysfunction Associated with Cardiovascular and Metabolic Diseases.” Vascular Pharmacology 100:1–19.

Kharroubi, Akram T., Hisham M. Darwish, Mutaz A. Akkawi, Abdelkareem A. Ashareef, Zaher A. Almasri, Khaldoun A. Bader, and Umaiyeh M. Khammash. 2015. “Total Antioxidant Status in Type 2 Diabetic Patients in Palestine.” Journal of Diabetes Research 2015.

Khemka, Vineet Kumar, Debajit Bagchi, Arindam Ghosh, Oishimaya Sen, Aritri Bir, Sasanka Chakrabarti, and Anindita Banerjee. 2013. “Raised Serum Adenosine Deaminase Level in Nonobese Type 2 Diabetes Mellitus.” The Scientific World Journal 2013.

Kumawat, Manjulata, Tarun Kumar Sharma, Ishwar Singh, Neelima Singh, Veena Singh Ghalaut, Satish Kumar Vardey, and Vijay Shankar. 2013. “Antioxidant Enzymes and Lipid Peroxidation in Type 2 Diabetes Mellitus Patients with and without Nephropathy.” North American Journal of Medical Sciences 5(3):213.

Kurtul, Naciye, Sadrettin Pence, Ersin Akarsu, Hasan Kocoglu, Yemliha Aksoy, and Hülya Aksoy. 2004. “Adenosine Deaminase Activity in the Serum of Type 2 Diabetic Patients.” ACTA MEDICA-HRADEC KRALOVE-. 47(1):33–36.

Larijani, Bagher, Ramin Heshmat, Mina Ebrahimi-Rad, Shohreh Khatami, Shirin Valadbeigi, and Reza Saghiri. 2016. “Diagnostic Value of Adenosine Deaminase and Its Isoforms in Type II Diabetes Mellitus.” Enzyme Research 2016.

Lemley, Kevin V, Isha Abdullah, Bryan D. Myers, Timothy W. Meyer, Kristina Blouch, William E. Smith, Peter H. Bennett, and Robert G. Nelson. 2000. “Evolution of Incipient Nephropathy in Type 2 Diabetes Mellitus.” Kidney International 58(3):1228–37.

Lu, Chun-feng, Wang-shu Liu, Xiao-qin Ge, and Feng Xu. 2021. “Serum Adenosine Deaminase Levels Are Associated with Diabetic Kidney Disease in Type 2 Diabetic Patients.” Endocrine Connections 10(9):973–79.

Marrocco, Ilaria, Fabio Altieri, and Ilaria Peluso. 2017. “Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans.” Oxidative Medicine and Cellular Longevity 2017.

Matough, Fatmah A., Siti B. Budin, Zariyantey A. Hamid, Nasar Alwahaibi, and Jamaludin Mohamed. 2012. “The Role of Oxidative Stress and Antioxidants in Diabetic Complications.” Sultan Qaboos University Medical Journal 12(1):5.

Murlidharan, Praveen, Sreelekshmi Kamaladevan, Satish Balan, and Chandrasekharan C. Kartha. 2020. “Mechanisms for Obesity Related Kidney Disease.” Pp. 193–216 in Pathophysiology of Obesity-Induced Health Complications. Springer.

Nagarajrao, Ramprasad, and Samir Abdulkarim Alharbi. 2018. “RELATIONSHIP BETWEEN OXIDANT AND ANTIOXIDANT ENZYMES STATUS IN TYPE 2 DIABETIC PATIENTS WITH NEPHROPATHY IN SAUDI POPULATION.” Asian Journal of Pharmaceutical and Clinical Research 363–68.

Najafi, Ali, Morteza Pourfarzam, and Fouzieh Zadhoush. 2021. “Oxidant/Antioxidant Status in Type-2 Diabetes Mellitus Patients with Metabolic Syndrome.” Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences 26.

Nakanishi, Shuhei, Hidenori Hirukawa, Masashi Shimoda, Fuminori Tatsumi, Kenji Kohara, Atsushi Obata, Seizo Okauchi, Yukino Katakura, Junpei Sanada, and Yoshiro Fushimi. 2019. “Comparison of HbA1c Levels and Body Mass Index for Prevention of Diabetic Kidney Disease: A Retrospective Longitudinal Study Using Outpatient Clinical Data in Japanese Patients with Type 2 Diabetes Mellitus.” Diabetes Research and Clinical Practice 155:107807.

Newsholme, Philip, Kevin N. Keane, Rodrigo Carlessi, and Vinicius Cruzat. 2019. “Oxidative Stress Pathways in Pancreatic β-Cells and Insulin-Sensitive Cells and Tissues: Importance to Cell Metabolism, Function, and Dysfunction.” American Journal of Physiology-Cell Physiology 317(3):C420–33.

Nwankwo, A. A., E. E. Osim, and S. A. Bisong. 2013. “Contributory Role of Adenosine Deaminase in Metabolic Syndrome.” Nigerian Journal of Physiological Sciences 28(1):73–76.

Pinnelli, Venkata Bharatkumar, C. A. Jayashankar, Shrabani Mohanty, G. Asha, Minu Mary Mathai, and D. S. Raghavendra. 2016. “Elevated Levels of Serum Adenosine Deaminase in Type 2 Diabetes Mellitus Patients.” International Journal of Research in Medical Sciences 4(1):131–34.

Pragada, Lavanya, Dhoom Singh Mehta, V. Manasa, Chandrahas Gangaram Bathini, Sneha Kesari, and Ritika Bansal. 2019. “Effect of Scaling and Root Planing on Gingival Crevicular Fluid Levels of Adrenomedullin in Chronic Periodontitis Patients with and without Diabetes Mellitus Type 2: A Clinico-Biochemical Study.” Annals of African Medicine 18(2):92.

Ramani, Nisha Subhashchandra, N. Krishnamurthy, N. Ashakiran, S. Sumathi, and R. Harish. 2012. “Role of Adenosine Deaminase to Predict Glycemic Status in Type 2 Diabetes Mellitus.” J Clin Biomed Sci 2(3):123–33.

Rao, Vikram, L. B. Vasanth Rao, Sean Hong Tan, Mayuren Candasamy, and Subrat Kumar Bhattamisra. 2019. “Diabetic Nephropathy: An Update on Pathogenesis and Drug Development.” Diabetes & Metabolic Syndrome: Clinical Research & Reviews 13(1):754–62.

Sapkota, Lokendra Bahadur, Sangita Thapa, and Nuwadatta Subedi. 2017. “Correlation Study of Adenosine Deaminase and Its Isoenzymes in Type 2 Diabetes Mellitus.” BMJ Open Diabetes Research and Care 5(1).

Singla, Heena, Gitanjali Goyal, Cheenu Garg, and Kajal Bhalla. 2019. “Total Antioxidant Status in Type 2 Diabetes Mellitus with Diabetic Nephropathy.”

Slatter, D. A., C. H. Bolton, and A. J. Bailey. 2000. “The Importance of Lipid-Derived Malondialdehyde in Diabetes Mellitus.” Diabetologia 43(5):550–57.

Sohn, Rebecca, Marius Junker, Andrea Meurer, Frank Zaucke, Rainer H. Straub, and Zsuzsa Jenei-Lanzl. 2021. “Anti-Inflammatory Effects of Endogenously Released Adenosine in Synovial Cells of Osteoarthritis and Rheumatoid Arthritis Patients.” International Journal of Molecular Sciences 22(16):8956.

Tao, Sibei, Wen Zheng, Yuan Liu, Ling Li, Lingzhi Li, Qian Ren, Min Shi, Jing Liu, Jing Jiang, and Huichao Ma. 2019. “Analysis of Serum Metabolomics among Biopsy-Proven Diabetic Nephropathy, Type 2 Diabetes Mellitus and Healthy Controls.” RSC Advances 9(33):18713–19.

Warjukar, Prajakta, Pradip Jain, Prakash Kute, Ashish Anjankar, and S. S. Ghangale. 2020. “Study of Microalbuminuria and Uric Acid in Type 2 Diabetes Mellitus.” Int J Cur Res Rev 2020.

Yap, Steven C., and H. Thomas Lee. 2012. “Adenosine and Protection from Acute Kidney Injury.” Current Opinion in Nephrology and Hypertension 21(1):24.