Studying the genotoxicity caused by sodium benzoate in liver cancer cell line (HEPG2) by comet assay
Keywords:
genotoxicity, sodium benzoate, HepG2 cancer cell, Comet assayAbstract
Objective: Genetic toxicity examines the health of those exposed to mutagenic agents and the mutagenic impacts of radiation and chemicals. The process of causing genetic alterations and DNA damage is known as mutagenesis. Therefore, it is vital to carry out research in this area at many levels and come up with suitable ways to stop or lessen the development of genetic toxicity. The sodium version of benzoic acid, sodium benzoate, is a common ingredient in both food and cosmetics. This study aims to explore the genotoxicity produced by sodium benzoate in the liver cancer cell line (HepG2) using the Comet assay because of the substance's widespread use. Method: The comet assay or single cell gel electrophoresis (SCGE) is a quick and accurate method for identifying and assessing DNA damage in single cells. One-way ANOVA and Tukey's multiple comparison posthoc test were used to examine the results, which were shown as the SD Mean of the report and the data connected to the Comet indices. The difference's significance threshold was set at p<0.0001. Findings: DNA damage was generated in the liver cancer cell line in the current investigation by sodium benzoate's genotoxicity at concentrations of 50, 100, and 150 micromolar (HepG2).
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