Medium optimization for extracellular urate oxidase production by a newly isolated Aspergillus Niger
Keywords:
Uricase, urate oxidase, optimization, Aspergillus nigerAbstract
Urate oxidase is a peroxisomal enzyme with four equal subunits that convert uric acid to allantoin, a more soluble metabolite for excretion. The usage of uricase as a drug in medicine is to treat hyperuricemia. Many microorganisms have been used for uricase production such as Streptomyces exfoliates, Pseudomonas aeruginosa, and Aspergillus flavus. In this study, soil samples were collected and then cultured in a screening medium including uric acid as the sole carbon source. Samples with the higher ability of uricase production were selected for enzyme assay. Enzyme activity was measured by spectrophotometry and the sample with the maximal uricase activity was identified as Aspergillus niger and selected for further studies. According to the results of experiments, the optimized temperature for enzyme production by Aspergillus niger was determined to be 35±2°C. The best carbon and nitrogen source was glucose and NH4NO3, and the highest enzyme activity was observed in the presence of Cu2+ ion.
Downloads
References
Nyborg, A.C., et al., A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties. PLoS One. 11(12), e0167935. (2016)
Forouzesh, M., Valipour, R., Shekari, A., Barzegar, A., & Setareh, M. Comparison of Methadone Level Measurement by Enzyme Immunoassay with Gas Chromatography-Mass Spectrometry. Journal of Advanced Pharmacy Education & Research| Oct-Dec. 9(4), 105-108. (2019).
Sargazi, M., & Taghian, F. The Effect of Royal Jelly and Exercise on Liver Enzymes in Addicts. Archives of Pharmacy Practice. 11(2), 96-101. (2020)
Tork S, Aly MM, Albureikan MO. New Tannase-Producing Lactobacillus Sp. Nrc10: Gene Cloning, Enzyme Purification, And Characterization. Pharmacophore. 10(5), 45-56. (2019)
Pustake, S.O., P.K. Bhagwat, and P.B. Dandge, Statistical media optimization for the production of clinical uricase from Bacillus subtilis strain SP6. Heliyon. 5(5), e01756. (2019)
Ghasemian, A., Z. Moradpour, M. Baniasad, and Y. Ghasemi, Isolation, molecular identification and characterization of the culture conditions for extracellular uricase production by a new strain of Pseudomonas sp. Journal of Pure and Applied Microbiology. 9(4), 2813-2821. (2015)
Shaaban, M.I., E. Abdelmegeed, and Y.M. Ali, Cloning, Expression, and Purification of Recombinant Uricase Enzyme from Pseudomonas aeruginosa Ps43 Using Escherichia coli. J Microbiol Biotechnol. 25(6), 887-92. (2015)
Fazel, R., N. Zarei, N. Ghaemi, M.M. Namvaran, S. Enayati, E. Mirabzadeh Ardakani, M. Azizi, and V. Khalaj, Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris. Springerplus. 3(1), 395. (2014)
Aly, M., S. Tork, S. Al-Garni, and R. Allam, Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes. Turkish Journal of Biology. 37(5), 520-529. (2013)
Pfrimer, P., L.M. de Moraes, A.S. Galdino, L.P. Salles, V.C. Reis, J.L. De Marco, M.V. Prates, C. Bloch, Jr., and F.A. Torres, Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli. J Biomed Biotechnol. 2010, 674908. (2010)
Tanaka, A., M. Yamamura, S. Kawamoto, and S. Fukui, Production of uricase by Candida tropicalis using n-alkane as a substrate. Appl Environ Microbiol. 34(4), 342-6. (1977)
Wu, X.W., C.C. Lee, D.M. Muzny, and C.T. Caskey, Urate oxidase: primary structure and evolutionary implications. Proc Natl Acad Sci U S A. 86(23), 9412-6. (1989)
Ames, B.N., R. Cathcart, E. Schwiers, and P. Hochstein, Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis. Proc Natl Acad Sci U S A. 78(11), 6858-62. (1981)
Jin, M., F. Yang, I. Yang, Y. Yin, J.J. Luo, H. Wang, and X.F. Yang, Uric acid, hyperuricemia and vascular diseases. Front Biosci (Landmark Ed). 17, 656-69. (2012)
Reinders, M.K. and T.L. Jansen, New advances in the treatment of gout: review of pegloticase. Ther Clin Risk Manag. 6, 543-50. (2010)
Vogt, B., Urate oxidase (rasburicase) for treatment of severe tophaceous gout. Nephrol Dial Transplant. 20(2), 431-3. (2005)
Tan, Q., et al., Uricase from Bacillus fastidious loaded in alkaline enzymosomes: enhanced biochemical and pharmacological characteristics in hypouricemic rats. Eur J Pharm Biopharm. 82(1), 43-8. (2012)
Rajoka, M.I., K.-u. Rehman, T. Tabish, and M.A. Zia, Purification and characterization of caprine kidney uricase, possessing novel kinetic and thermodynamic properties. World Journal of Microbiology and Biotechnology. 22(3), 289-291. (2005)
Rainbird, R.M. and C.A. Atkins, Purification and some properties of urate oxidase from nitrogen-fixing nodules of cowpea. Biochimica et Biophysica Acta (BBA) - Enzymology. 659(1), 132-140. (1981)
Alishah, K., S. Asad, K. Khajeh, and N. Akbari, Utilizing intein-mediated protein cleaving for purification of uricase, a multimeric enzyme. Enzyme Microb Technol. 93-94, 92-98. (2016)
Zhang, C., X. Yang, J. Feng, Y. Yuan, X. Li, Y. Bu, Y. Xie, H. Yuan, and F. Liao, Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus. Biosci Biotechnol Biochem. 74(6), 1298-301. (2010)
Ohe, T. and Y. Watanabe, Purification and properties of urate oxidase from Streptomyces cyanogenus. J Biochem. 89(6), 1769-76. (1981)
Suzuki, K., S. Sakasegawa, H. Misaki, and M. Sugiyama, Molecular cloning and expression of uricase gene from Arthrobacter globiformis in Escherichia coli and characterization of the gene product. J Biosci Bioeng. 98(3), 153-8. (2004)
Lotfy, W.A., Production of a thermostable uricase by a novel Bacillus thermocatenulatus strain. Bioresour Technol. 99(4), 699-702. (2008)
Chen, M., L. Cai, Z. Fang, H. Tian, X. Gao, and W. Yao, Site-specific incorporation of unnatural amino acids into urate oxidase in Escherichia coli. Protein Sci. 17(10), 1827-33. (2008)
Geweely, N.S. and L.S. Nawar, Production, optimization, purification and properties of uricase isolated from some fungal flora in Saudi Arabian soil. Australian Journal of Basic and Applied Sciences. 5(10), 220-230. (2011)
Liu, J., G. Li, H. Liu, and X. Zhou, Purification and properties of uricase from Candida sp. and its application in uric acid analysis in serum. Appl Biochem Biotechnol. 47(1), 57-63. (1994)
Itaya, K., T. Yamamoto, and J. Fukumoto, Studies on Yeast Uricase. Agricultural and Biological Chemistry. 31(11), 1256-1264. (2014)
Kotb, E., Characterization of a Thermostable Uricase Isolated from Bacillus firmus DWD-33 and its Application for Uric Acid Quantification in Human Serum. Protein Pept Lett. 22(5), 402-9. (2015)
Bongaerts, G.P., J. Uitzetter, R. Brouns, and G.D. Vogels, Uricase of Bacillus fastidiosus. Properties and regulation of synthesis. Biochim Biophys Acta. 527(2), 348-58. (1978)
Kahn, K. and P.A. Tipton, Kinetic mechanism and cofactor content of soybean root nodule urate oxidase. Biochemistry. 36(16), 4731-8. (1997)
Juan, E.C., et al., Structures of Arthrobacter globiformis urate oxidase-ligand complexes. Acta Crystallogr D Biol Crystallogr. D64(Pt 8), 815-22. (2008)
Colloc'h, N., et al., Oxygen pressurized X-ray crystallography: probing the dioxygen binding site in cofactorless urate oxidase and implications for its catalytic mechanism. Biophys J. 95(5), 2415-22. (2008)
Yazdi, M.T., G. Zarrini, E. Mohit, M.A. Faramarzi, N. Setayesh, N. Sedighi, and F.A. Mohseni, Mucor hiemalis: a new source for uricase production. World Journal of Microbiology and Biotechnology. 22(4), 325-330. (2006)
Ravichandran, R., S. Hemaasri, S.S. Cameotra, and N.S. Jayaprakash, Purification and characterization of an extracellular uricase from a new isolate of Sphingobacterium thalpophilum (VITPCB5). Protein Expr Purif. 114, 136-42. (2015)
Laboureur, P. and C. Langlois, [Urate oxidase of Aspergillus flavus. I. Isolation, purification, properties]. Bull Soc Chim Biol (Paris). 50(4), 811-25. (1968)
Tan, Q.Y., et al., Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes. Int J Nanomedicine. 7, 3929-38. (2012)
Rajoka, M.I., K.U. Rehman, M. Mehraj, M.W. Akhtar, and M.A. Zia, Purification, and properties of a bovine uricase. Protein Pept Lett. 13(4), 363-8. (2006)
Amirthanathan, A. and S. Vijayakumar, Purification and optimization of uricase enzyme produced by Pseudomonas aeruginosa. Journal of Experimental Sciences. (2011)
Abdel-Fattah, G. and N. Abo-Hamed, Bioconversion of poultry wastes I-Factors influencing the assay and productivity of crude uricase by three uricolytic filamentous fungi. Acta microbiologica et immunologica Hungarica. 49(4), 445-454. (2002)
Ali, U.F. and Z.M. Ibrahim, Effect of irradiation on uricase produced by two strains of Asperigillus niger. Life Science Journal. 10(1), 962-967. (2013)
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
