Comparative analysis of fatty acid profiles through transesterification in macroalgae from Gulf of Mannar reveal Ulva lactuca for potent edible oil synthesis

Ashwini Karuppaswamy; Jayavel Sridhar; M. Pandi

doi:10.53730/ijhs.v6nS8.13503

Authors

Ashwini Karuppaswamy
ashmicrobiology2021@gmail.com
Department of Biotechnology, Madurai Kamaraj University, Madurai-625021
Jayavel Sridhar Department of Biotechnology, Madurai Kamaraj University, Madurai-625021
M. Pandi Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-625021

Keywords:

Ulva lactuca, fatty acid profile, edible oil, nutraceuticals, transesterification

Abstract

Selective macroalgae comprising green algae (Ulva lactuca), brown algae (Padina gymnosphora, Sargassam wightii, Turbinaria ornate) and red algae (Gracilaria corticata, Halimeda macrolopa, Halymenia dilatata, Gracilaria crassa) were collected from Gulf of Mannar, India. Taxonomic and molecular identification affirmed the identity of the microalgal species. Free fatty acids and Fatty Acid Methyl Ester (FAME) synthesis by transesterification was assessed for cataloguing the edible oil source from the various macroalgae collected. Lipid components were extracted employing various solvents namely, Hexane, Chloroform: methanol (2:1), Chloroform: hexane (1:1), Chloroform: hexane (1:2) and Dichloromethane: methanol (2:1). The effects of different solvents based on Soxhlet extraction were preceded and GC-MS was utilized for cataloguing the various lipid components in the macroalgae. Furthermore, in vitro anticancer activity against the hepatocellular carcinoma cell line, HepG2 indicate 15.6µg/ml concentration showed significant cytotoxicity compared to control. Thus, the present report emphasizes the optimal role of Ulva lactuca as a potential edible oil source supplement, along with pharmaceutical and neutraceutical benefits.

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References

Arjun, P. 2011. Investigations on microprogation and isolation of active compounds from Tribulus terrestris Linn. Ph D thesis, Faculty of Sciences, University of Madras, Chennai.

Arjun, P., Semwal, D.K., Semwal, R.B., Malaisamy M., Sivaraj C., & Vijayakumar, S. (2017). Total Phenolic Content, Volatile Constituents and Antioxidative Effect of Coriandrum sativum, Murrayakoenigii and Mentha arvensis. The Natural Products Journal, 7(1), 65-74.

Bhuyar, P., Sundararaju, S., Rahim, M. H. A., Unpaprom, Y., Maniam, G. P., & Govindan, N. (2021). Antioxidative study of polysaccharides extracted from red (Kappaphycus alvarezii), green (Kappaphycus striatus) and brown (Padina gymnospora) marine macroalgae/seaweed. SN Applied Sciences, 3(4), 1-9.

Breure, M. S. (2014). Exploring the potential for using seaweed (Ulva lactuca) as organic fertilizer (Doctoral dissertation, MSc Thesis Plant Production Systems. PPS-80436, Wageningen University. Netherlands).

Dominguez, H., & Loret, E. P. (2019). Ulva lactuca, a source of troubles and potential riches. Marine drugs, 17(6), 357.

García-Poza, S., Morais, T., Leandro, A., Cotas, J., Marques, J. C., Pereira, L., & Gonçalves, A. M. (2022). A Comparative Study of the Fatty Acids and Monosaccharides of Wild and Cultivated Ulva sp. Journal of Marine Science and Engineering, 10(2), 233.

Gaubert, J., Payri, C. E., Vieira, C., Solanki, H., & Thomas, O. P. (2019). High metabolic variation for seaweeds in response to environmental changes: a case study of the brown algae Lobophora in coral reefs. Scientific reports, 9(1), 1-12.

Gurusamy, S., Kulanthaisamy, M. R., Hari, D. G., Veleeswaran, A., Thulasinathan, B., Muthuramalingam, J. B., & Alagarsamy, A. (2019). Environmental friendly synthesis of TiO2-ZnO nanocomposite catalyst and silver nanomaterials for the enhanced production of biodiesel from Ulva lactuca seaweed and potential antimicrobial properties against the microbial pathogens. Journal of Photochemistry and Photobiology B: Biology, 193, 118-130.

Hidalgo, P., Ciudad, G., & Navia, R. (2016). Evaluation of different solvent mixtures in esterifiable lipids extraction from microalgae Botryococcus braunii for biodiesel production. Bioresource technology, 201, 360-364.

Lee, S. Y., Khoiroh, I., Vo, D. V. N., Senthil Kumar, P., & Show, P. L. (2021). Techniques of lipid extraction from microalgae for biofuel production: a review. Environmental Chemistry Letters, 19(1), 231-251.

Moreira, A. S., da Costa, E., Melo, T., Lopes, D., Pais, A., Santos, S. A., & Domingues, M. R. (2021). Polar lipids of commercial Ulva spp. of different origins: Profiling and relevance for seaweed valorization. Foods, 10(5), 914.

Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55-63.

Nunes, N., Rosa, G. P., Ferraz, S., Barreto, M. C., & de Carvalho, M. A. A. (2020). Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira. Journal of Applied Phycology, 32(2), 759-771.

Otero, P., Quintana, S. E., Reglero, G., Fornari, T., & García-Risco, M. R. (2018). Pressurized liquid extraction (PLE) as an innovative green technology for the effective enrichment of Galician algae extracts with high quality fatty acids and antimicrobial and antioxidant properties. Marine drugs, 16(5), 156.

Pangestuti, R., Haq, M., Rahmadi, P., & Chun, B. S. (2021). Nutritional Value and Biofunctionalities of Two Edible Green Seaweeds (Ulva lactuca and Caulerpa racemosa) from Indonesia by Subcritical Water Hydrolysis. Marine Drugs, 19(10), 578.

Pourkarimi, S., Hallajisani, A., Alizadehdakhel, A., & Nouralishahi, A. (2021). Bio-oil production by pyrolysis of Azolla filiculoides and Ulva fasciata macroalgae. Global Journal of Environmental Science and Management, 7(3), 331-346.

Rocha, C. P., Pacheco, D., Cotas, J., Marques, J. C., Pereira, L., & Gonçalves, A. M. (2021). Seaweeds as valuable sources of essential fatty acids for human nutrition. International Journal of Environmental Research and Public Health, 18(9), 4968.

Roleda, M. Y., Lage, S., Aluwini, D. F., Rebours, C., Brurberg, M. B., Nitschke, U., & Gentili, F. G. (2021). Chemical profiling of the Arctic sea lettuce Ulva lactuca (Chlorophyta) mass-cultivated on land under controlled conditions for food applications. Food Chemistry, 341, 127999.

Satpati, G. G., Kanjilal, S., Narayana Prasad, R. B., & Pal, R. (2015). Rapid accumulation of total lipid in Rhizoclonium africanum Kutzing as biodiesel feedstock under nutrient limitations and the associated changes at cellular level. International Journal of Microbiology.

Sinurat, E., & Fransiska, D. (2021). The Effect of Addition Glycerol Against Nori Characterization from Gracilaria sp and Ulva sp Seaweeds. In IOP Conference Series: Earth and Environmental Science (Vol. 715, No. 1, p. 012054) IOP Publishing.

Subramanian, N., Mahendradas, D. K., Kasirajan, R., & Sahadevan, R. (2015). Bio-oil separation from potential non-edible urban waste source Putranjiva roxburghii. Separation Science and Technology, 50(13), 2066-2074.

Uribe, E., Vega-Gálvez, A., García, V., Pastén, A., López, J., & Goñi, G. (2019). Effect of different drying methods on phytochemical content and amino acid and fatty acid profiles of the green seaweed, Ulva spp. Journal of Applied Phycology, 31(3), 1967-1979.