Phytochemical and antioxidant screening of Cocculus hirsutus and Calycopteris floribunda
Keywords:
Cocculus hirsutus, Calycopteris floribunda, antioxidant, total phenolic content, total flavonoid contentAbstract
Cocculus hirsutus and Calycopteris floribunda are two important Indian medicinal plants that are having multiple health benefits in traditional medicine. The current research aims to investigate the qualitative and quantitative phytochemical screening of ethanolic extracts of the selected plants using standard protocols. The Total phenolic content and total flavonoid content were estimated by Folin–Ciocalteu & Aluminum chloride colorimetric methods respectively. Invitro antioxidant activity by DPPH and NO free radical scavenging assay. Results unveiled that the total phenolic content for the ethanolic extract of C. hirsutus and C. floribunda are 157.46±1.12 & 113.25±1.03 GAE per gram of sample in dry weight (mg/g) and total flavonoid content was 69±1.05 & 58±1.23 of RE per gram of sample in dry weight (mg/g) respectively. In the DPPH assay, ethanolic extract of C. hirsutus is effective to inhibit (71.02%) the DPPH free radicals with IC50 values 48.10µg/ml at higher doses that are comparable to standard ascorbic acid (79.26%, IC50 =38.39µg/ml) followed by C. floribunda (65.24%, IC50 =60.49µg/ml). Similarly, in the NO free radical scavenging assay, C. hirsutus (75.19%) exhibited the highest free radical scavenging activity with IC50 values of 44.43µg/ml followed by C. floribunda (71.02%, IC50 =52.14µg/ml).
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