Phytochemical and antioxidant screening of Cocculus hirsutus and Calycopteris floribunda

Saidulu Abbagoni; Sujatha Edupuganti; G. Jhansi Rani

doi:10.53730/ijhs.v5nS1.13642

Authors

Saidulu Abbagoni Department of Botany, Osmania University, Telangana, India, 500007
Sujatha Edupuganti
sujatha@osmania.ac.in
Department of Botany, Osmania University, Telangana, India, 500007
G. Jhansi Rani Department of Botany, University college of Science Saifabad, Osmania University, Telangana, India, 500007

Keywords:

Cocculus hirsutus, Calycopteris floribunda, antioxidant, total phenolic content, total flavonoid content

Abstract

Cocculus hirsutus and Calycopteris floribunda are two important Indian medicinal plants that are having multiple health benefits in traditional medicine. The current research aims to investigate the qualitative and quantitative phytochemical screening of ethanolic extracts of the selected plants using standard protocols. The Total phenolic content and total flavonoid content were estimated by Folin–Ciocalteu & Aluminum chloride colorimetric methods respectively. Invitro antioxidant activity by DPPH and NO free radical scavenging assay. Results unveiled that the total phenolic content for the ethanolic extract of C. hirsutus and C. floribunda are 157.46±1.12 & 113.25±1.03 GAE per gram of sample in dry weight (mg/g) and total flavonoid content was 69±1.05 & 58±1.23 of RE per gram of sample in dry weight (mg/g) respectively. In the DPPH assay, ethanolic extract of C. hirsutus is effective to inhibit (71.02%) the DPPH free radicals with IC₅₀ values 48.10µg/ml at higher doses that are comparable to standard ascorbic acid (79.26%, IC₅₀=38.39µg/ml) followed by C. floribunda (65.24%, IC₅₀=60.49µg/ml). Similarly, in the NO free radical scavenging assay, C. hirsutus (75.19%) exhibited the highest free radical scavenging activity with IC₅₀ values of 44.43µg/ml followed by C. floribunda (71.02%, IC₅₀=52.14µg/ml).

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